Suppressive subtractive hybridization analysis of Rhipicephalus (Boophilus) microplus larval and adult transcript expression during attachment and feeding

Lew-Tabor, A.E.; Moolhuijzen, Paula; Vance, Megan E.; Kurscheid, Sebastian; Rodriguez-Valle, M.; Jarrett, S.J.; Minchin, Catherine M.; Jackson, Louise A.; Jonsson, N.N.; Bellgard, M.; Guerrero, Felix D.

doi:10.1016/j.vetpar.2009.09.033

Cited by 29 publications

(30 citation statements)

References 53 publications

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“…These proteins have been shown to be involved in the cellular response to different stress conditions such as heat shock (HSPs; [18, 19, 21]), endogenous and environmental chemicals (GST; [41]), oxidative stress (SEL, MET, FER1; [42–44]), and metals (MET, FER1; [43, 44]). Additionally, these proteins have been reported to be regulated by tick attachment, blood feeding, or pathogen infection [4, 5, 14, 15, 45–51] as well as expressed in unfed and uninfected ticks and tick cells [10, 11, 52, 53]. …”

Section: Resultsmentioning

confidence: 99%

Expression of Heat Shock and Other Stress Response Proteins in Ticks and Cultured Tick Cells in Response toAnaplasmaspp. Infection and Heat Shock

Villar

Ayllón

Busby

et al. 2010

International Journal of Proteomics

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Ticks are ectoparasites of animals and humans that serve as vectors of Anaplasma and other pathogens that affect humans and animals worldwide. Ticks and the pathogens that they transmit have coevolved molecular interactions involving genetic traits of both the tick and the pathogen that mediate their development and survival. In this paper, the expression of heat shock proteins (HSPs) and other stress response proteins (SRPs) was characterized in ticks and cultured tick cells by proteomics and transcriptomics analyses in response to Anaplasma spp. infection and heat shock. The results of these studies demonstrated that the stress response was activated in ticks and cultured tick cells after Anaplasma spp. infection and heat shock. However, in the natural vector-pathogen relationship, HSPs and other SRPs were not strongly activated, which likely resulted from tick-pathogen coevolution. These results also demonstrated pathogen- and tick-specific differences in the expression of HSPs and other SRPs in ticks and cultured tick cells infected with Anaplasma spp. and suggested the existence of post-transcriptional mechanisms induced by Anaplasma spp. to control tick response to infection. These results illustrated the complexity of the stress response in ticks and suggested a function for the HSPs and other SRPs during Anaplasma spp. infection.

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Section: Resultsmentioning

confidence: 99%

Expression of Heat Shock and Other Stress Response Proteins in Ticks and Cultured Tick Cells in Response toAnaplasmaspp. Infection and Heat Shock

Villar

Ayllón

Busby

et al. 2010

International Journal of Proteomics

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“…Tick HSPs and other SRPs such as glutathione- S -transferase, selenoproteins, metallothioneins, and ferritin have been shown to be involved in the cellular response to different stress conditions such as heat shock, oxidative stress, tick attachment, blood feeding, and pathogen infection (Macaluso et al, 2003; Mulenga et al, 2003, 2007; Rudenko et al, 2005; Ribeiro et al, 2006; de la Fuente et al, 2007b; Rachinsky et al, 2007, 2008; Hajdusek et al, 2009; Zivkovic et al, 2009; Kongsuwan et al, 2010; Lew-Tabor et al, 2010; Villar et al, 2010; Busby et al, 2012). For example, recent studies demonstrated that the stress response is activated in ticks and cultured tick cells after Anaplasma spp.…”

Section: Impact Of Weather-induced Tick Stress Response On Tick Survimentioning

confidence: 99%

Impact of Climate Trends on Tick-Borne Pathogen Transmission

Estrada-Peña¹,

Ayllón²,

Fuente³

2012

Front. Physio.

217

144

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Recent advances in climate research together with a better understanding of tick–pathogen interactions, the distribution of ticks and the diagnosis of tick-borne pathogens raise questions about the impact of environmental factors on tick abundance and spread and the prevalence and transmission of tick-borne pathogens. While undoubtedly climate plays a role in the changes in distribution and seasonal abundance of ticks, it is always difficult to disentangle factors impacting on the abundance of tick hosts from those exerted by human habits. All together, climate, host abundance, and social factors may explain the upsurge of epidemics transmitted by ticks to humans. Herein we focused on tick-borne pathogens that affect humans with epidemic potential. Borrelia burgdorferi s.l. (Lyme disease), Anaplasma phagocytophilum (human granulocytic anaplasmosis), and tick-borne encephalitis virus (tick-borne encephalitis) are transmitted by Ixodes spp. Crimean–Congo hemorrhagic fever virus (Crimean–Congo hemorrhagic fever) is transmitted by Hyalomma spp. In this review, we discussed how vector tick species occupy the habitat as a function of different climatic factors, and how these factors impact on tick survival and seasonality. How molecular events at the tick–pathogen interface impact on pathogen transmission is also discussed. Results from statistically and biologically derived models are compared to show that while statistical models are able to outline basic information about tick distributions, biologically derived models are necessary to evaluate pathogen transmission rates and understand the effect of climatic variables and host abundance patterns on pathogen transmission. The results of these studies could be used to build early alert systems able to identify the main factors driving the subtle changes in tick distribution and seasonality and the prevalence of tick-borne pathogens.

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“…This approach allowed us to identify 40 genes that were differentially upregulated or induced in response to attainment of appetence and/or exposure to feeding stimuli (Mulenga et al, 2007). Since our previous study (Mulenga et al, 2007), two similar studies that used new generation sequencing approaches to identify genes associated with regulating the initial phases of Rhipicephalus microplus tick feeding have been described (Lew-Tabor et al, 2010; Rodriguez-Valle et al, 2010). …”

Section: Introductionmentioning

confidence: 99%

“…Implementation of this strategy was made possible due to the availability of multiple expressed sequence tag (EST) and RNA sequencing research outputs for Ambylomma tick spp. (Nene et al, 2002; Aljamali et al, 2009; Karim et al, 2011), other tick species (Hill and Guttierrez, 2000; Valenzuella et al, 2002; Nene et al, 2004; Santos et al, 2004; Guerrero etal., 2005; Ribeiro et al, 2006; Wang et al, 2007; Anatriello et al, 2010; Lees et al, 2010; Lew-Tabor et al, 2010; Rodriguez-Valle et al, 2010; Francischetti et al, 2011) and the Ixodes scapularis genome sequence data (Pagel et al, 2007), that allowed us to identify some of tick orphan genes that are cross-tick species conserved (Mulenga et al, 2007). In the current study the goal was to deorphanize and gain insight into the significance and role(s) of the A. americanum ( Aam ) AV422 gene in tick feeding regulation.…”

Section: Introductionmentioning

confidence: 99%

Deorphanization and target validation of cross-tick species conserved novel Amblyomma americanum tick saliva protein

Mulenga

Kim

Ibelli

2013

International Journal for Parasitology

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We previously identified a cross-tick species conserved tick feeding stimuli responsive Amblyomma americanum (Aam) AV422 gene. This study demonstrates that AamAV422 belongs to a novel group of arthropod proteins that is characterized by 14 cysteine amino acid residues: C23-X7/9-C33-X23/24-C58-C8-C67X7-X75-X23-C99-X15-C115-X10-C126X24/25/33-C150C151-X7-C159-X8-X168-X23/24-C192-X9/10-C202 predicted to form seven disulfide bonds. We show that AamAV422 protein is a ubiquitously expressed protein that is injected into the host within the first 24 h of the tick attaching onto the host as revealed by western blotting analyses of recombinant (r)AamAV422, tick saliva and dissected tick organ protein extracts using antibodies to 24 h and 48 h tick saliva proteins (TSPs). Native AamAV422 is apparently involved with mediating tick anti-hemostasis and anti-complement functions in that rAamAV422 delayed plasma clotting time in a dose responsive manner by up to ~160 s, prevented platelet aggregation by up to ~16% and caused ~24% reduction in production of terminal complement complexes. Target validation analysis revealed that rAamAV422 is a potential candidate for a cocktail or multivalent tick vaccine preparation in that RNA interference (RNAi)-mediated silencing of AamAV422 mRNA caused a statistically significant (~44%) reduction in tick engorgement weights, which is proxy for amounts of ingested blood. We speculate that AamAV422 is a potential target antigen for development of the highly desired universal tick vaccine in that consistent with high conservation among ticks, antibodies to 24 h Ixodes scapularis TSPs specifically bound rAamAV422. We discuss data in this study in the context of advancing the biology of tick feeding physiology and discovery of potential target antigens for tick vaccine development.

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Suppressive subtractive hybridization analysis of Rhipicephalus (Boophilus) microplus larval and adult transcript expression during attachment and feeding

Cited by 29 publications

References 53 publications

Expression of Heat Shock and Other Stress Response Proteins in Ticks and Cultured Tick Cells in Response toAnaplasmaspp. Infection and Heat Shock

Expression of Heat Shock and Other Stress Response Proteins in Ticks and Cultured Tick Cells in Response toAnaplasmaspp. Infection and Heat Shock

Impact of Climate Trends on Tick-Borne Pathogen Transmission

Deorphanization and target validation of cross-tick species conserved novel Amblyomma americanum tick saliva protein

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