Supramolecular Carbon Monoxide‐Releasing Peptide Hydrogel Patch

Abstract

Carbon monoxide (CO) is recently accepted as a therapeutic molecule that exhibits remarkable biological actions, including anti-inflammation, antiapoptosis, and cytoprotection, at a physiological level. For clinical use without the side effect of tissue hypoxia, which arises from the uncontrolled administration of CO in the human body, CO-releasing molecules (CORMs) are developed to ensure safe and efficient CO-delivery. Herein, a syringe-injectable CO-releasing peptide hydrogel (COH) and a corresponding bioadhesive hydrogel patch (COHP), developed by rational supramolecular chemistry, to enhance the therapeutic efficacy of CO with controllable CO-release to a specific tissue is report. The injectable COH is prepared by self-assembly of the CORM-attached peptides with a gel-forming diphenylalanine-derivative, resulting in fibrillar networks and exhibiting prolonged CO-release compared with CORMs. Furthermore, Ca 2+ -chelating and mussel-derived catechol-functionalized peptides are introduced to afford a mechanically rigid, bioadhesive COHP that elicits cytoprotective and anti-inflammatory activities. The supramolecular COHP can be utilized in the efficient CO-delivery to the site of interest by conformal contacts, making it a promising scaffold for biomedical applications.

Design of therapeutic biomaterials to control inflammation

“…Alternatively, esterase and pH-responsive CO prodrugs can be administered for the treatment of LPS-induced systemic inflammation in a mouse model 220 . Prolonged CO release can be achieved by a syringe-injectable peptide hydrogel, enabling a CO-releasing half-lifetime of 20.2 ± 0.6 min, which is longer than the commonly used CO-releasing molecule Ru(CO) 3 Cl(glycinate) ( t 1/2 ≈ 3.6 min) 221 .…”

Metal-free carbon monoxide-releasing micelles undergo tandem photochemical reactions for cutaneous wound healing

“…[7][8][9][10] These CORMs can be roughly categorized into two sorts, namely metal carbonyls and metal-free CORMs. Although metal carbonyls hold great promise in both laboratory and clinical investigations, 9,[11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26] the development of metal-free CORMs has recently been receiveing increasing attention. 27 In this context, boranocarbonates, 28 CO-releasing prodrugs operated in a "click and release" manner, [29][30][31] and photoresponsive organic CORMs such as 3-hydroxyavone (3-HF) derivatives, [32][33][34][35][36][37] xanthene-9-carboxylic acid, 38 BODIPY derivatives, 39 and aromatic a-diketone derivatives 40 have been developed.…”

Engineering Injectable Anti‐Inflammatory Hydrogels to Treat Acute Myocardial Infarction

“…In this study, the author coupled the CO‐releasing molecule tricarbonylchloro(glycinato)ruthenium(II) (CORM‐3, Ru(CO) 3 Cl(glycinate)) to the peptide based on FF, followed by self‐assembly with fluorenylmethoxycarbonyl (Fmoc)‐FF to form an injectable hydrogel. [ 210 ] This CO‐releasing peptide‐based hydrogel showed effective cytoprotective and anti‐inflammatory effects on oxidatively stressed cardiomyocytes. Therefore, the use of hydrogels to achieve locally controlled release of CO may be another strategy to inhibit the inflammatory response in AMI.…”