Supramolecular Chiro-Biomedical Assays and Enantioselective HPLC Analyses for Evaluation of Profens as Non-Steroidal Anti-Inflammatory Drugs, Potential Anticancer Agents and Common Xenobiotics

Abstract

The permanent world-wide increase in therapeutic administration of racemic profens as easy available non-prescribed analgesic drugs and a common first-choice anti-inflammatory agents was recently linked with renewed interest in their beneficial use, also as enantiopure formulations, to treat and/or prevent a variety of human malignancies including its four major types as colorectal, breast, lung, and prostate cancer. This underlies the continuous need of selecting perfectly suited chiral separation methods of profens capable to determine nanolevels of a distomer in presence of the eutomer in a variety of complex biological and environmental media. Thus, current improvements for direct enantiomeric separations of profens by well defined supramolecular-based chiral HPLC and recently developed monolithic, combinatorial, bimodal and polymeric chiral stationary phases employing a modern supramolecular chirality concepts has been outlined in this review. The use of diverse supramolecular approaches for chiral HPLC as an easy accessible tool enabling fast development of nanoscale enantioselective, high-throughput and gradient screening procedures for in situ monitoring of stereoselective ADME properties of profens in range of anticancer drug discovery technologies has been also addressed.

Development and validation of SPMMTE HPLC method for analysis of profens from human plasma

“…The profens show their pharmaceutical activities by inhibiting biosynthesis of prostaglandin into the human body. The profens competitively inhibit biosynthesis of cyclo‐oxygenase, an enzyme that catalyzes the synthesis of prostaglandin (Ali et al , ). The most commonly used profens for the treatment of rheumatoid arthritis and osteoarthritis are ibuprofen, ketoprofen, and flurbiprofen (Fig.…”

The differential cell signaling effects of two positional isomers of the anticancer NO-donating aspirin

“…Isomerism can influence drug activity, at times profoundly (13)(14)(15). Our study, focused on the m-and p-positional isomers of NO-ASA, establishes a striking difference in biological activity between them.…”

“…Isomerism is of particular interest to pharmacology as it can influence drug activity, at times profoundly (13)(14)(15). There are four main types of drug isomers, positional isomers, geometric isomers, enantiomers and diastereoisomers.…”

Chiral Separations: Fundamental Review 2010

“…Current improvements for direct enantiomeric separation of profens by HPLC including monolithic, combinatorial, bimodal, and polymeric CSPs was reviewed (35).…”