Supramolecular Nanofibers and Hydrogels of Nucleopeptides

Li, Xinming; Kuang, Yi; Lin, Hsin‐Chieh; Gao, Yuan; Shi, Junfeng; Xu, Bing

doi:10.1002/anie.201103641

Cited by 138 publications

(138 citation statements)

References 60 publications

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“…The neutral peptide-like backbone replacing the negatively charged phosphodiester groups, which may limit self-association due to the repulsion of similar charges, adds structural elasticity and chemical adaptability. Another group of unnatural peptide-nucleobases hybrids (denoted nucleopeptides) have already been shown to self-assemble into nanofibres to generate supramolecular hydrogels 20 . Despite the great potential to converge the two distinct fields of peptide self-assembly and structural DNA nanotechnology, no work has been performed on PNAs as building blocks to assemble into distinct structural entities by themselves.…”

mentioning

confidence: 99%

Light-emitting self-assembled peptide nucleic acids exhibit both stacking interactions and Watson–Crick base pairing

et al. 2015

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The two main branches of bionanotechnology involve the self-assembly of either peptides or DNA. Peptide scaffolds offer chemical versatility, architectural flexibility and structural complexity, but they lack the precise base pairing and molecular recognition available with nucleic acid assemblies. Here, inspired by the ability of aromatic dipeptides to form ordered nanostructures with unique physical properties, we explore the assembly of peptide nucleic acids (PNAs), which are short DNA mimics that have an amide backbone. All 16 combinations of the very short di-PNA building blocks were synthesized and assayed for their ability to self-associate. Only three guanine-containing di-PNAs-CG, GC and GG-could form ordered assemblies, as observed by electron microscopy, and these di-PNAs efficiently assembled into discrete architectures within a few minutes. The X-ray crystal structure of the GC di-PNA showed the occurrence of both stacking interactions and Watson-Crick base pairing. The assemblies were also found to exhibit optical properties including voltage-dependent electroluminescence and wide-range excitation-dependent fluorescence in the visible region.

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mentioning

confidence: 99%

Light-emitting self-assembled peptide nucleic acids exhibit both stacking interactions and Watson–Crick base pairing

et al. 2015

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“…The reason why PC12 was used for the MTT test is that PC12 is a neuron-like cell and the application of DPSS chips is for neurostimulation. After incubation for 24 h, the medium was replaced with fresh medium supplemented with MTT (4 mg mL -1 , (28)(29)(30) 0.5 mL per well). After another 4 h, the medium containing MTT was removed and dimethyl sulfoxide (DMSO) (0.5 mL per well) was added to dissolve the formazan crystals.…”

Section: Irox Process On Electrodesmentioning

confidence: 99%

“…The optical density of the resulting solution was measured with a 595 nm light source using an absorbance microplate reader (Infinite F50, TECAN). (28)(29)(30) The cell viability percentage was calculated by the following formula:…”

Section: Irox Process On Electrodesmentioning

confidence: 99%

A CMOS 256-pixel Photovoltaics-powered Implantable Chip with Active Pixel Sensors and Iridium-oxide Electrodes for Subretinal Prostheses

Wu¹,

Kuo²,

Lin³

et al. 2018

Sensors and Materials

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Keywords: divisional power supply scheme, active pixel sensor, photovoltaic cell, CMOS image sensor, biocompatible package A CMOS implantable chip with 256 active pixel sensors (APSs), on-chip photovoltaic cells, and iridium-oxide (IrOx) electrodes is proposed and designed for subretinal prostheses. In the proposed chip, the on-chip electrode surface is deposited with IrOx by RF sputtering and photolithography patterning. The divisional power supply scheme (DPSS) is adopted to generate sufficient stimulation current whereas the APS is used to enhance the image sensitivity. The proposed chip consists of a 16 × 16 photodiode array with 8 DPSS divisions, control signal generator circuits, and photovoltaic cells. It is designed and fabricated into 180 nm CMOS image sensor (CIS) technology and postprocessed with an IrOx coating. From the electrical measurement results, the fabricated chip has a peak output stimulation current of 16.7 μA under the equivalent electrode impedance load. The stimulation frequency is 30.2 Hz and the amount of injected charges at each pixel is 3.5 nC. Both image light sensitivity and injection charges are significantly improved. The surface morphology, crystallinity, charge storage capacity, and biocompatibility of sputtering iridium oxide film (SIROF) were investigated. As a result, the SIROF has desirable physical and electrochemical properties that make it suitable for the neurostimulation electrodes on the 256-pixel implantable chip. The 7-month biocompatibility and charge delivery capability of the fabricated chip have been confirmed by electroretinography (ERG) measurement on a Lanyu minipig in in vivo animal tests.

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“…It is well-known that spermidine acts as a multivalent cation and can form rods and toroids in vitro [44]. Li and coworkers have reported the formation of nanofibers using bis(tert-butyloxycarbonyl) (bis-Boc) protected adenine and (N 6 -bis-Boc-adenine-9-yl)-acetic acid, which formed via stacking interactions of the aromatic adenine base [45]. Thus, similar interactions are likely to be involved in the FVBS assemblies, where in two of the amino groups on the spermidine chain are protected by the boc group, while the third forms an amide linkage with the carboxyl group of the Fmocvaline moiety.…”

Section: Formation Of Fvbs Assembliesmentioning

confidence: 99%

Supramolecular Fmoc-valyl based nanoassemblies for delivery of mitoxantrone into HeLa cells

Romanelli

Fath

Davidov

et al. 2015

Journal of Drug Delivery Science and Technology

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Supramolecular Nanofibers and Hydrogels of Nucleopeptides

Cited by 138 publications

References 60 publications

Light-emitting self-assembled peptide nucleic acids exhibit both stacking interactions and Watson–Crick base pairing

Light-emitting self-assembled peptide nucleic acids exhibit both stacking interactions and Watson–Crick base pairing

A CMOS 256-pixel Photovoltaics-powered Implantable Chip with Active Pixel Sensors and Iridium-oxide Electrodes for Subretinal Prostheses

Supramolecular Fmoc-valyl based nanoassemblies for delivery of mitoxantrone into HeLa cells

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