Supramolecular Nanovalves Controlled by Proton Abstraction and Competitive Binding

Leung, Ken Cham Fai; Nguyen, The-Vinh; Zink, Jeffrey I.

doi:10.1021/cm061682d

Cited by 193 publications

(120 citation statements)

References 53 publications

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“…[1][2][3][4] Furthermore, recent reports on the design of functional mesoporous silica materials by decorating the pore surface with organic or inorganic moieties that could serve as gating devices to regulate the release of guest molecules under the control of several different external stimuli, such as chemicals, [5][6][7][8][9] temperature, 10 redox reactions, 11,12 and photoirradiation, 13,14 have highlighted the potential of utilizing this kind of nanodevice for many controlled release applications.…”

Section: Introductionmentioning

confidence: 99%

Mesoporous Silica Nanoparticles for Intracellular Delivery of Membrane-Impermeable Proteins

2007

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An MCM-41-type mesoporous silica nanoparticle (MSN) material with a large average pore diameter (5.4 nm) is synthesized and characterized. The in vitro uptake and release profiles of cytochrome c by the MSN were investigated. The enzymatic activity of the released protein was quantitatively analyzed and compared with that of the native cytochrome c in physiological buffer solutions. We found that the enzymes released from the MSNs are still functional and highly active in catalyzing the oxidation of 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) by hydrogen peroxide. In contrast to the fact that cytochrome c is a cell-membrane-impermeable protein, we discovered that the cytochrome c-encapsulated MSNs could be internalized by live human cervical cancer cells (HeLa) and the protein could be released into the cytoplasm. We envision that these MSNs with large pores could serve as a transmembrane delivery vehicle for controlled release of membrane-impermeable proteins in live cells, which may lead to many important biotechnological applications including therapeutics and metabolic manipulation of cells. Abstract: An MCM-41-type mesoporous silica nanoparticle (MSN) material with a large average pore diameter (5.4 nm) is synthesized and characterized. The in vitro uptake and release profiles of cytochrome c by the MSN were investigated. The enzymatic activity of the released protein was quantitatively analyzed and compared with that of the native cytochrome c in physiological buffer solutions. We found that the enzymes released from the MSNs are still functional and highly active in catalyzing the oxidation of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS) by hydrogen peroxide. In contrast to the fact that cytochrome c is a cell-membrane-impermeable protein, we discovered that the cytochrome c-encapsulated MSNs could be internalized by live human cervical cancer cells (HeLa) and the protein could be released into the cytoplasm. We envision that these MSNs with large pores could serve as a transmembrane delivery vehicle for controlled release of membrane-impermeable proteins in live cells, which may lead to many important biotechnological applications including therapeutics and metabolic manipulation of cells.

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Section: Introductionmentioning

confidence: 99%

Mesoporous Silica Nanoparticles for Intracellular Delivery of Membrane-Impermeable Proteins

2007

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“…Thirdly, nitrogen-sorption measurements performed before and after the formation of the AuNC@BSA shell around cargo-free MSN showed the disappearance of the type IV isotherm with a sharp drop of the Brunauer-Emmet-Teller (BET) surface area, from 863 to 162 m 2 .g -1 ( Figure 2D). The diameter of the pores of silica NPs was centered at 2.3 nm (Figure S9), [76] which is lower than that of the gold-protein bioconjugates. Fourthly, the coloration of capped NPs was obvious ( Figure 2E) and the inductively coupled plasma-optical emission spectroscopy AuNC@BSA (see Figure 2G).…”

Section: Preparation Of Msn-aunc@bsa Nanocarriersmentioning

confidence: 92%

Protein-gold clusters-capped mesoporous silica nanoparticles for high drug loading, autonomous gemcitabine/doxorubicin co-delivery, and in-vivo tumor imaging

Croissant¹,

Zhang

Alsaiari

et al. 2016

Journal of Controlled Release

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154

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, Protein-gold clusters-capped mesoporous silica nanoparticles for high drug loading, autonomous gemcitabine/doxorubicin co-delivery, and in-vivo tumor imaging, Journal of Controlled Release (2016Release ( ), doi: 10.1016Release ( /j.jconrel.2016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT

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“…In the realm of drug delivery, a sophisticated nanodelivery system, which is capable of storing and protecting drug molecules, controlling and regulating release of cargo molecules only at target site, is unequivocally desired. In this regard, release of the drugs or active agents from nanocontainers had been successfully demonstrated to be regulated in response to changes in redox potential, 17 pH, 18,19 temperature, 20 light, 21 and the presence of enzymes found in certain diseases. 22 The rate of release of the cargo molecules relies on the shape and geometrical properties of nanocontainers.…”

Section: Introductionmentioning

confidence: 99%

In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages

Li¹,

Yoong²,

Goh³

et al. 2015

IJN

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Carbon nanotubes’ (CNTs) hollow interior space has been explored for biomedical applications, such as drug repository against undesirable inactivation. To further devise CNTs as smart material for controlled release of cargo molecules, we propose the concept of “gold-carbon nanobottles”. After encapsulating cis -diammineplatinum(II) dichloride (cisplatin, CDDP) in CNTs, we covalently attached gold nanoparticles (AuNPs) at the open-tips of CNTs via different cleavable linkages, namely hydrazine, ester, and disulfide-containing linkages. Compared with our previous study in which more than 80% of CDDP leaked from CNTs in 2 hours, AuNPs were found to significantly decrease such spontaneous release to <40%. In addition, CDDP release from AuNP-capped CNTs via disulfide linkage was selectively enhanced by twofolds in reducing conditions (namely with 1 mM dithiothreitol [DTT]), which mimic the intracellular environment. We treated human colon adenocarcinoma cells HCT116 with our CDDP-loaded gold-carbon nanobottles and examined the cell viability using lactate dehydrogenase assay. Interestingly, we found that our nanobottles with cleavable disulfide linkage exerted stronger cytotoxic effect in HCT116 compared with normal human fetal lung fibroblast cells IMR-90. Therefore, we infer that our nanobottles strategy with inbuilt disulfide linkage could attain selective release of payload in highly reductive tumor tissues while avoiding collateral damage to normal tissues.

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Supramolecular Nanovalves Controlled by Proton Abstraction and Competitive Binding

Cited by 193 publications

References 53 publications

Mesoporous Silica Nanoparticles for Intracellular Delivery of Membrane-Impermeable Proteins

Mesoporous Silica Nanoparticles for Intracellular Delivery of Membrane-Impermeable Proteins

Protein-gold clusters-capped mesoporous silica nanoparticles for high drug loading, autonomous gemcitabine/doxorubicin co-delivery, and in-vivo tumor imaging

In vitro controlled release of cisplatin from gold-carbon nanobottles via cleavable linkages

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