Sur la structure du polysaccharide des cites D d'une souche humaine virulente de mycobacterium tuberculosis

Sur la structrue chimique des cires D, peptidoglycolipides macromoléculaires des souches humaines de Mycobacterium tuberculosis

“…Nous avons entrepris 1'8tude des cires D d'une souche humaine de Nycobacterium tuberculosis [7] et avons decrit recemment la structure des oligosaccharides et des glycolipides obtenus au cours de leur degradation [8,9].…”

“…Aprbs leur isolement et purification par chromatographies successives sur acide silicique-c&te (2 : 1) et precipitations au moyen des solvants organiques [7,8] …”

Isolation and Characterization of Arabinose Mycolate from Firmly Bound Lipids of Mycobacteria

“…We also reported previously that arabinose mycolate was obtained from wax D fraction (5) and defatted cell wall fraction (9) of human tubercle bacillus strain Aoyama B after treatment with 0.1 N hydrochloric acid solution at 37 C. These results were confirmed by Acharya et al (1) with the cell wall and wax D fractions of M. tuberculosis strain BCG and M. kansaii. More recently, it was suggested that a mycolic acid-arabinogalactan-mucopeptide complex might be a common structure of mycobacterial cell wall (9,11) and wax D (6,17) fractions. Since bound lipid and wax D fractions are mainly located in the cell wall (13), D-arabinose-5-mycolate purified from firmly bound lipid, wax D and cell wall fractions of mycobacterial strains may indicate the combining site of mycolic acid with the arabinogalactan portion of mycobacterial wax D and cell wall fractions.…”

Mycobacterial adjuvant

“…Raffel [3] and Shinozaki et al [4] showed that the adjuvant activity of a tubercle bacillus was attributable only to wax D among various lipid fractions. Several attempts [2,5,8] had been made to isolate an adjuvant-active component from wax D, but the active substance had not been confirmed as a single component. We have described in this report a new preparative method for an adjuvant-active component from wax D by thin-layer chromatography as a single spot.…”