SURE gel electrophoresis: A method for improved detection and purification of dilute nucleic acid samples

Sowersby, Drew S.; Lewis, L. Kevin

doi:10.1016/j.ab.2023.115373

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…The DNA tetrahedron was characterized by 6% polyacrylamide gel electrophoresis (PAGE) at 120 V for 40 min . The results are represented in Figure below.…”

Section: Resultsmentioning

confidence: 99%

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Highly Efficient Photoelectrochemical Detection of Cystatin C Based on a Core–Shell MOF Nanocomposite with Biomimetic-Catalysis Amplification

Xia,

Yang,

Zhu

et al. 2024

ACS Omega

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“…The DNA tetrahedron was characterized by 6% polyacrylamide gel electrophoresis (PAGE) at 120 V for 40 min . The results are represented in Figure below.…”

Section: Resultsmentioning

confidence: 99%

“…The DNA tetrahedron was characterized by 6% polyacrylamide gel electrophoresis (PAGE) at 120 V for 40 min. 28 The results are represented in Figure 2 below. From left to right in Figure 2 , the six lanes were the Marker band, single-stranded S 1 , S 2 , S 3 , and S 4 , and the synthesized DNA tetrahedron.…”

Section: Resultsmentioning

confidence: 99%

Highly Efficient Photoelectrochemical Detection of Cystatin C Based on a Core–Shell MOF Nanocomposite with Biomimetic-Catalysis Amplification

Xia,

Yang,

Zhu

et al. 2024

ACS Omega

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“…Gel electrophoresis based on agarose gel is considered to be the most effective method of separating DNA fragments, and it can analyze DNA fragments of varying sizes [1]. In general, electrophoresis of DNA is used for separating DNA fragments based on DNA sizes [2][3][4]; however, conventional gel electrophoresis is limited in throughput and requires a substantial amount of time and skills to monitor DNA fragments based on their size [5,6]. In conventional gel electrophoresis based on agarose gel, the DNA sample is loaded into pre-cast wells, and a current is applied to assist DNA fragment migration in agarose gel.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Analysis of Genomic DNA Degradation of E. coli Using Automated Gel Electrophoresis under Various Levels of Microwave Exposure

Pandey,

Momeni,

Pandey

2024

Gels

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The problem that this study addresses is to understand how microwave radiation is able to degrade genomic DNA of E. coli. In addition, a comparative study was made to evaluate the suitability of a high-throughput automated electrophoresis platform for quantifying the DNA degradation under microwave radiation. Overall, this study investigated the genomic DNA degradation of E. coli under microwave radiation using automated gel electrophoresis. To examine the viable organisms and degradation of genomic DNA under microwave exposure, we used three methods: (1) post-microwave exposure, where E. coli was enumerated using modified mTEC agar method using membrane filtration technique; (2) extracted genomic DNA of microwaved sample was quantified using the Qubit method; and (3) automated gel electrophoresis, the TapeStation 4200, was used to examine the bands of extracted DNA of microwaved samples. In addition, to examine the impacts of microwaves, E. coli colonies were isolated from a fecal sample (dairy cow manure), these colonies were grown overnight to prepare fresh E. coli culture, and this culture was exposed to microwave radiation for three durations: (1) 2 min; (2) 5 min; and (3) 8 min. In general, Qubit values (ng/µL) were proportional to the results of automated gel electrophoresis, TapeStation 4200, DNA integrity numbers (DINs). Samples from exposure studies (2 min, 5 min, and 8 min) showed no viable E. coli. Initial E. coli levels (at 0 min microwave exposure) were 5 × 108 CFU/mL, and the E. coli level was reduced to a non-detectable level within 2 min of microwave exposure. The relationships between Qubit and TapeStation measurements was linear, except for when the DNA level was lower than 2 ng/µL. In 8 min of microwave exposure, E. coli DNA integrity was reduced by 61.7%, and DNA concentration was reduced by 81.6%. The overall conclusion of this study is that microwave radiation had a significant impact on the genomic DNA of E. coli, and prolonged exposure of E. coli to microwaves can thus lead to a loss of genomic DNA integrity and DNA concentrations.

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Sample loading in gel electrophoresis using adapted 3D printers

Tao,

Kopyl,

Yew

et al. 2025

Analytical Biochemistry

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SURE gel electrophoresis: A method for improved detection and purification of dilute nucleic acid samples

Cited by 4 publications

References 26 publications

Highly Efficient Photoelectrochemical Detection of Cystatin C Based on a Core–Shell MOF Nanocomposite with Biomimetic-Catalysis Amplification

Highly Efficient Photoelectrochemical Detection of Cystatin C Based on a Core–Shell MOF Nanocomposite with Biomimetic-Catalysis Amplification

Quantitative Analysis of Genomic DNA Degradation of E. coli Using Automated Gel Electrophoresis under Various Levels of Microwave Exposure

Sample loading in gel electrophoresis using adapted 3D printers

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