Surface acoustic wave-based lab-on-a-chip for the fast detection of Legionella pneumophila in water

Gagliardi, Mariacristina; Agostini, Matteo; Lunardelli, Francesco; Lamanna, Leonardo; Miranda, Alessio; Bazzichi, Agostino; Luminare, Antonella Giuliana; Cervelli, Fabrizio; Gambineri, Francesca; Totaro, Michele; Lai, Michele; Maisetta, Giuseppantonio; Batoni, Giovanna; Pistello, Mauro; Cecchini, Marco

doi:10.1016/j.snb.2023.133299

Cited by 17 publications

(5 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The water sample was enriched with both gram-positive and gram-negative bacteria, as well as anti-legionella antibodies for the specific identification of the targeted cells. The LOD that this device achieved was 2.01 ∗ 10 6 cfu/mL and the microchamber was fabricated using PDMS [ 140 ].…”

Section: Microfluidics and Locs In Food Safety Applicationsmentioning

confidence: 99%

Polymeric and Paper-Based Lab-on-a-Chip Devices in Food Safety: A Review

2023

View full text Add to dashboard Cite

Food quality and safety are important to protect consumers from foodborne illnesses. Currently, laboratory scale analysis, which takes several days to complete, is the main way to ensure the absence of pathogenic microorganisms in a wide range of food products. However, new methods such as PCR, ELISA, or even accelerated plate culture tests have been proposed for the rapid detection of pathogens. Lab-on-chip (LOC) devices and microfluidics are miniaturized devices that can enable faster, easier, and at the point of interest analysis. Nowadays, methods such as PCR are often coupled with microfluidics, providing new LOC devices that can replace or complement the standard methods by offering highly sensitive, fast, and on-site analysis. This review’s objective is to present an overview of recent advances in LOCs used for the identification of the most prevalent foodborne and waterborne pathogens that put consumer health at risk. In particular, the paper is organized as follows: first, we discuss the main fabrication methods of microfluidics as well as the most popular materials used, and then we present recent literature examples for LOCs used for the detection of pathogenic bacteria found in water and other food samples. In the final section, we summarize our findings and also provide our point of view on the challenges and opportunities in the field.

show abstract

Section: Microfluidics and Locs In Food Safety Applicationsmentioning

confidence: 99%

Polymeric and Paper-Based Lab-on-a-Chip Devices in Food Safety: A Review

2023

View full text Add to dashboard Cite

show abstract

“…Compared to conventional methods, LOC offer unique features such as compact, automation capabilities, and proficiency in executing multiplexed analyses using tiny sample volumes. This innovative design streamlines sample processing, significantly reduce the time for analysis and enhance the detection efficiency (Gagliardi et al, 2023). With the capacity to combine multiple detection techniques such as PCR, immunoassays, and nucleic acid sequencing within one unified platform, LOC technologies empower users to conduct numerous assessments of water quality almost instantly.…”

Section: Lab-on-chip and Microfluidic Technologies For Waterborne Pat...mentioning

confidence: 99%

Waterborne pathogens detection technologies: advances, challenges, and future perspectives

Oon,

Ayaz

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

The World Health Organization (WHO) estimated that pathogens like Escherichia coli, primarily linked to food and water contamination, are associated with 485,000 deaths from diarrheal diseases annually, translating to a staggering worldwide economic loss of nearly 12 billion USD per annum. International organizations like the WHO and United Nations Children’s Fund (UNICEF) have established related guidelines and criteria for pathogenic detection technologies and driving the search for innovative and efficient detection methods. This comprehensive review examines the trajectory of waterborne pathogenic bacteria detection technologies from traditional techniques, i.e., culture-based methods, to current detection methods including various forms of polymerase chain reaction (PCR) techniques [qualitative real-time PCR, digital PCR, ELISA, loop-mediated isothermal amplification, next-generation sequencing (NGS)] and to emerging techniques, i.e., biosensors and artificial intelligence (AI). The scope of the review paper focuses on waterborne pathogenic bacteria that are recognized as human pathogens, posing tangible threats to public health through waterborne. The detection techniques’ merits, constraints, research gaps and future perspectives are critically discussed. Advancements in digital droplet PCR, NGS and biosensors have significantly improved sensitivity and specificity, revolutionizing pathogen detection. Additionally, the integration of artificial intelligence (AI) with these technologies has enhanced detection accuracy, enabling real-time analysis of large datasets. Molecular-based methods and biosensors show promise for efficient water quality monitoring, especially in resource-constrained settings, but on-site practical implementation remains a challenge. The pairwise comparison metrics used in this review also offer valuable insights into quick evaluation on the advantages, limitations and research gaps of various techniques, focusing on their applicability in field settings and timely analyses. Future research efforts should focus on developing robust, cost-effective and user-friendly techniques for routine waterborne bacteria monitoring, ultimately safeguarding global water supplies and public health, with AI and data analysis playing a crucial role in advancing these methods for a safer environment.

show abstract

“…Polymeric flexible SAW device finds their natural outgrowth in the development of biosensor and Lab on a chip. [11,108] Portable, miniaturized, and compliant devices are required not only in healthcare monitoring but even in the food chain, to monitor contamination and food ripening, and even as support for environmental and agriculture monitoring. [109][110][111] From this perspective, the development of polymeric SAW devices lags behind conventional SAW devices that have achieved remarkable advancements in recent years.…”

Section: Biosensors and Microfluidicsmentioning

confidence: 99%

Recent Progress in Polymeric Flexible Surface Acoustic Wave Devices: Materials, Processing, and Applications

Lamanna

2023

Adv Materials Technologies

Self Cite

View full text Add to dashboard Cite

Recently, there has been a remarkable increase in interest in piezoelectric thin films, particularly zinc oxide (ZnO) and aluminum nitride (AlN), deposited on flexible polymeric substrates. This is due to the rapid expansion of fields such as robotics, wearable devices, and the Internet of Things (IoT). These thin‐film layered structures have a wide range of applications, such as energy harvesting, sensing and biosensing, microfluidic sorting, pumping and mixing, and telecommunications. One of the most promising platforms is piezoelectric acoustic‐based thin‐film devices on inexpensive, recyclable, or disposable polymeric substrates. Their reliability, reproducibility, conformability, small size, and wireless control capabilities make them a leading candidate for the development of new wireless, fully automated, and digitized microsystems for IoT and wearable devices. This review examines recent advancements in the engineering of ZnO and AlN thin films on polymeric, flexible, and conformable surface acoustic wave (SAW) devices. Topics covered include the materials used, piezoelectric film deposition, device fabrication, and the latest applications of this technology as sensors and actuators.

show abstract

Surface acoustic wave-based lab-on-a-chip for the fast detection of Legionella pneumophila in water

Cited by 17 publications

References 40 publications

Polymeric and Paper-Based Lab-on-a-Chip Devices in Food Safety: A Review

Polymeric and Paper-Based Lab-on-a-Chip Devices in Food Safety: A Review

Waterborne pathogens detection technologies: advances, challenges, and future perspectives

Recent Progress in Polymeric Flexible Surface Acoustic Wave Devices: Materials, Processing, and Applications

Contact Info

Product

Resources

About