Surface‐Active Fluorinated Quantum Dots for Enhanced Cellular Uptake

Argudo, Pablo G.; Carril, Mónica; Martín‐Romero, María T.; Giner‐Casares, Juan J.; Carrillo‐Carrión, Carolina

doi:10.1002/chem.201804704

Cited by 12 publications

(10 citation statements)

References 39 publications

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“…Additionally, insufficient information is available in the experimental literature, exploring the bionanointeractions, and there is no consensus on the toxicity of nanoscale materials that exhibit the unique nanocell interface behavior when compared to their bulk counterparts . Several studies have highlighted since the past few years the key factors, which potentially determine the fate of nanomedicine in cell-specific diagnostic and therapy, encompassing the physicochemical characteristics − of nanoparticles, such as size, geometry, surface functionalization and charge, colloidal stability in a biological medium, interaction with cells, and cellular uptake and trafficking as well as delivery − to the cellular targets.…”

Section: Introductionmentioning

confidence: 99%

Orange-Emitting ZnSe:Mn²⁺ Quantum Dots as Nanoprobes for Macrophages

Khan

Uchiyama

Khan³

et al. 2020

ACS Appl. Nano Mater.

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The biocompatibility, bionanointeraction, uptake efficiency, and entry pathway of luminescent nanomaterials are the key factors to understand development of an efficient bionanoprobe. The foremost objective of this work is to explore the potential of 3-mercaptopropionic acid (3-MPA) capped ZnSe:xMn 2+ (x = 5, 10, and 15 mol %) quantum dots (QDs) for the development of bionanoprobe used in future biological and clinical applications. For this purpose, highly intense orangeemitting activator Mn 2+ ion doped ZnSe QDs were synthesized via a high-temperature organometallic method and rendered watersoluble by a ligand exchange approach. The morphological and physicochemical characterizations displayed the ultrasmall zincblend cubic crystal structure of QDs with an elliptical shape nanocrystals and average diameter of 4 nm. The luminescent nanomaterials exhibited orange emission centered at 584 nm under excitation at 385 nm. The biocompatibility, time-dependent cellular uptake, and the uptake mechanism of QDs were studied in RAW 264.7 macrophages, accomplished by various cytotoxicity assays, CytoViva hyperspectral enhanced dark-field and dual-mode fluorescence (DMF) microscopy, and transmission electron microscopy (TEM) images. The cytotoxicity study did not confirm any noticeable deleterious effect of QDs within incubation for 6 h. The fluorescence images of cells incubated with QDs showed efficient emission, which is a manifestation that QDs are photochemically stable in the intracellular environment. The cellular uptake findings demonstrated that the QDs were predominantly internalized via clathrin-and caveolae-mediated pathways. After the uptake, QDs aggregates appeared inside the vesicles in the cytoplasm, and their number and size gradually increased as a function of time. Nevertheless, the fluorescent QDs presented remarkable colloidal stability in various media, biocompatibility within the designated time, efficient time-dependent uptake, and distinct entry pathway in RAW macrophages, suggesting promising candidates to explore for the development of future bionanoprobes.

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Section: Introductionmentioning

confidence: 99%

Orange-Emitting ZnSe:Mn²⁺ Quantum Dots as Nanoprobes for Macrophages

Khan

Uchiyama

Khan³

et al. 2020

ACS Appl. Nano Mater.

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“…On the one hand, fluorinated dendrimers were reported to effectively enhance genetic material delivery by means of an increased uptake and fast endosomal escape [87,88]. On the other hand, fluorinated QDs were internalized more than equivalent non-fluorinated QDs, as observed by confocal microscopy, although this might be due to the fluorine-mediated association of QDs, which led to bigger-sized structures [89,90]. In any case, the role of fluorine on NPs in protein corona formation and how this affects interactions with cells is yet to be unveiled.…”

Section: Fluorinated Hydrophobic Coatingsmentioning

confidence: 99%

Recent Developments in the Design of Non-Biofouling Coatings for Nanoparticles and Surfaces

Sanchez‐Cano

Carril

2020

IJMS

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Biofouling is a major issue in the field of nanomedicine and consists of the spontaneous and unwanted adsorption of biomolecules on engineered surfaces. In a biological context and referring to nanoparticles (NPs) acting as nanomedicines, the adsorption of biomolecules found in blood (mostly proteins) is known as protein corona. On the one hand, the protein corona, as it covers the NPs’ surface, can be considered the biological identity of engineered NPs, because the corona is what cells will “see” instead of the underlying NPs. As such, the protein corona will influence the fate, integrity, and performance of NPs in vivo. On the other hand, the physicochemical properties of the engineered NPs, such as their size, shape, charge, or hydrophobicity, will influence the identity of the proteins attracted to their surface. In this context, the design of coatings for NPs and surfaces that avoid biofouling is an active field of research. The gold standard in the field is the use of polyethylene glycol (PEG) molecules, although zwitterions have also proved to be efficient in preventing protein adhesion and fluorinated molecules are emerging as coatings with interesting properties. Hence, in this review, we will focus on recent examples of anti-biofouling coatings in three main areas, that is, PEGylated, zwitterionic, and fluorinated coatings.

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“…Rotello noncovalently incorporated dyes or drugs into zwitterionic Active targeting is a strategy to provide enhanced cellular uptake by utilizing affinity ligands on the NP surface for specific recognition with receptors overexpressed on target cells [92,93]. Small molecule ligands (e.g., folic acid, methotrexate, anisamide, cholic acid, daptomycin, fluorine and sugars) have been utilized extensively for conjugation to various inorganic NPs due to their stability, ease of modification and availability [94][95][96]. To date, several therapeutic and diagnostic approaches using active targeting NPs have entered clinical trials, with several gaining FDA approval [97].…”

Section: Modulating Np-cell Interactionsmentioning

confidence: 99%

Engineering the Interface between Inorganic Nanoparticles and Biological Systems through Ligand Design

et al. 2021

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Nanoparticles (NPs) provide multipurpose platforms for a wide range of biological applications. These applications are enabled through molecular design of surface coverages, modulating NP interactions with biosystems. In this review, we highlight approaches to functionalize nanoparticles with ”small” organic ligands (Mw < 1000), providing insight into how organic synthesis can be used to engineer NPs for nanobiology and nanomedicine.

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Surface‐Active Fluorinated Quantum Dots for Enhanced Cellular Uptake

Cited by 12 publications

References 39 publications

Orange-Emitting ZnSe:Mn²⁺ Quantum Dots as Nanoprobes for Macrophages

Orange-Emitting ZnSe:Mn²⁺ Quantum Dots as Nanoprobes for Macrophages

Recent Developments in the Design of Non-Biofouling Coatings for Nanoparticles and Surfaces

Engineering the Interface between Inorganic Nanoparticles and Biological Systems through Ligand Design

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Surface‐Active Fluorinated Quantum Dots for Enhanced Cellular Uptake

Cited by 12 publications

References 39 publications

Orange-Emitting ZnSe:Mn2+ Quantum Dots as Nanoprobes for Macrophages

Orange-Emitting ZnSe:Mn2+ Quantum Dots as Nanoprobes for Macrophages

Recent Developments in the Design of Non-Biofouling Coatings for Nanoparticles and Surfaces

Engineering the Interface between Inorganic Nanoparticles and Biological Systems through Ligand Design

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Product

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Orange-Emitting ZnSe:Mn²⁺ Quantum Dots as Nanoprobes for Macrophages

Orange-Emitting ZnSe:Mn²⁺ Quantum Dots as Nanoprobes for Macrophages