Surface and adsorption characteristics of three elastin-like polypeptide coatings with varying sequence lengths

Srokowski, Elizabeth M.; Woodhouse, Kimberly A.

doi:10.1007/s10856-012-4772-6

Cited by 17 publications

(17 citation statements)

References 50 publications

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“…Interestingly, both the control PET‐coated and ELP1‐coated sensors displayed comparable levels of adsorbed Fg suggesting that ELP1 may not be as effective in resisting Fg adsorption as the other ELPs. Fibrinogen is known to have high surface activity so it is also possible that Fg may have displaced some of the ELP coating, especially with the shorter ELPs that appear to have lower coating stability …”

Section: Resultsmentioning

confidence: 99%

“…Fibrinogen is known to have high surface activity so it is also possible that Fg may have displaced some of the ELP coating, especially with the shorter ELPs that appear to have lower coating stability. 35 To gain insight into possible differences in the viscoelastic properties of the adsorbed protein layers (e.g., those induced by conformational changes), specific dissipation 37,38 defined as the DD/DF was evaluated for the ELP adsorbed layers. Lower values of specific dissipation indicate a more rigid compact film whereas higher values can be attributed to adsorbed films that are more hydrated with an expanded flexible conformation.…”

Section: Fibrinogen Adsorption Properties On the Family Of Elp Coatingsmentioning

confidence: 99%

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Evaluation of the bulk platelet response and fibrinogen interaction to elastin‐like polypeptide coatings

Srokowski

Woodhouse

2013

J Biomedical Materials Res

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In this work, we expand on our understanding of the thrombogenicity of coatings prepared with three different recombinant elastin-like polypeptides (ELPs). The bulk platelet response of the ELP coatings was characterized following whole blood contact under physiological shear flow (300 s(-1) ) using flow cytometry. Prolonged exposure to shear flow (1-h) indicated that materials coated with the longer ELP coatings (ELP2 and ELP4) had less bulk platelet activation and microparticle formation than materials coated with the shorter ELP1. Quartz crystal microbalance with dissipation (QCM-D) was used to monitor the binding of the platelet membrane receptor GPIIb/IIIa to ELP-adsorbed fibrinogen (Fg) surfaces. Compared to the shorter ELPs, a lower amount of Fg adsorbed to the ELP4 coated material and ELP4 appeared to form a softer, more structurally flexible coating layer. When Fg was adsorbed to the ELP coated surface it demonstrated an altered binding for GPIIb/IIIa that was inhibited in the presence of an AGDV-containing peptide but not an RGD-containing peptide. Conversely, on the shorter ELP coatings, binding of GPIIb/IIIa to an adsorbed Fg layer was partially inhibited in the presence of an RGD-containing peptide. These results indicate that both the quantity and conformational state of Fg varies when adsorbed to surfaces coated with ELPs of varying sequence length, which may be mediating their platelet response. Collectively, the findings reinforce the applicability of the ELPs as potential thromboresistant coatings, especially with the use of the longer polypeptide-ELP4.

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Section: Resultsmentioning

confidence: 99%

Section: Fibrinogen Adsorption Properties On the Family Of Elp Coatingsmentioning

confidence: 99%

Evaluation of the bulk platelet response and fibrinogen interaction to elastin‐like polypeptide coatings

Srokowski

Woodhouse

2013

J Biomedical Materials Res

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“…On the day of seeding, half of the samples were statically coated with 100 uL of a 1 mg/mL ELP4 solution in 70% ethanol for one hour. ELP4 surface concentration was determined based on previous work [29] . Cells, from passage 6–10, were statically seeded on one side of the scaffold at a cell density of 50,000 cells/scaffold in non-tissue culture treated 96 well polystyrene plates.…”

Section: Methodsmentioning

confidence: 99%

Electrospun fiber constructs for vocal fold tissue engineering: Effects of alignment and elastomeric polypeptide coating

et al. 2015

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Vocal fold lamina propria extracellular matrix (ECM) is highly aligned and when injured, becomes disorganized with loss of the tissue’s critical biomechanical properties. This study examines the effects of electrospun fiber scaffold architecture and elastin-like polypeptide (ELP4) coating on human vocal fold fibroblast (HVFF) behavior for applications toward tissue engineering the vocal fold lamina propria. Electrospun Tecoflex™ scaffolds were made with aligned and unaligned fibers, and were characterized using scanning electron microscopy and uniaxial tensile testing. ELP4 was successfully adsorbed onto the scaffolds; HVFF were seeded and their viability, proliferation, morphology, and gene expression were characterized. Aligned and unaligned scaffolds had initial elastic moduli of ~14 MPa, ~5 MPa and ~0.3 MPa, ~0.6 MPa in the preferred and cross-preferred directions, respectively. Scaffold topography had an effect on the orientation of the cells, with HVFF seeded on aligned scaffolds having a significantly different (p < 0.001) angle of orientation than HVFF cultured on unaligned scaffolds. This same effect and significant difference (p < 0.001) was seen on aligned and unaligned scaffolds coated with ELP4. Scaffold alignment and ELP4 coating impacted ECM gene expression. ELP4 coating, and aligned scaffolds upregulated elastin synthesis when tested on day 7 without a concomitant upregulation of collagen III synthesis. Collectively, results indicate that aligned electrospun scaffolds and ELP4 coating, are promising candidates in the development of biodegradeable vocal fold lamina propria constructs.

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“…In other words, the longest polypeptides formed films with greater structural flexibility and associated water content. The dynamic nature of the adsorbed layer may be an important factor that contributes to its biological response [76]. Additionally, a decrease in fibrinogen accretion and platelet adhesion was observed for ELR-coated compared to uncoated surfaces, with the longest ELR coatings exhibiting lower levels of fibrinogen adsorption and platelet adhesion and therefore being the best thromboresistant coatings [77].…”

Section: Biofunctionality Control Using Elrsmentioning

confidence: 99%

Recent Contributions of Elastin-Like Recombinamers to Biomedicine and Nanotechnology

Arias¹,

Santos²,

Fernández‐Colino³

et al. 2014

CTMC

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Abstract:The emergence of the new scientific field known as nanomedicine is being catalyzed by multiple improvements in nanoscience techniques and significant progress in materials science, especially as regards the testing of novel and sophisticated biomaterials. This conjuncture has furthered the development of promising instruments in terms of detection, bioanalysis, therapy, diagnostics and imaging. Some of the most innovative new biomaterials are protein-inspired biomimetic materials in which modern biotechnology and genetic-engineering techniques complement the huge amount of information afforded by natural protein evolution to create advanced and tailor-made multifunctional molecules. Amongst these protein-based biomaterials, Elastin-like Recombinamers (ELRs) have demonstrated their enormous potential in the fields of biomedicine and nanoscience in the last few years. This broad applicability derives from their unmatched properties, particularly their recombinant and tailor-made nature, the intrinsic characteristics derived from their elastin-based origin (mainly their mechanical properties and ability to self-assemble as a result of their stimuli-responsive behavior), their proven biocompatibility and biodegradability, as well as their versatility as regards incorporating advanced chemical or recombinant modifications into the original structure that open up an almost unlimited number of multifunctional possibilities in this developing field. This article provides an updated review of the recent challenges overcome by using these recombinant biomaterials in the fields of nano-and biomedicine, ranging from nanoscale applications in surface modifications and self-assembled nanostructures to drug delivery and regenerative medicine.

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Surface and adsorption characteristics of three elastin-like polypeptide coatings with varying sequence lengths

Cited by 17 publications

References 50 publications

Evaluation of the bulk platelet response and fibrinogen interaction to elastin‐like polypeptide coatings

Evaluation of the bulk platelet response and fibrinogen interaction to elastin‐like polypeptide coatings

Electrospun fiber constructs for vocal fold tissue engineering: Effects of alignment and elastomeric polypeptide coating

Recent Contributions of Elastin-Like Recombinamers to Biomedicine and Nanotechnology

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