Surface and internal ultrastructure of hemocytes of some insects

Akai, Hiromu; Satô, Syôiti

doi:10.1017/cbo9780511759987.006

Cited by 14 publications

(7 citation statements)

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“…Yeager (1945), using Prodenia eridania, has identified 32 types of haemocytes categorized into 10 classes. The transmission and scanning electron microscopic studies of Akai and Sato (1971, 1973, 1976 confirmed the presence of five types of haemocytes in B. mori and the same number holds true for Locusta migratoria in addition to the presence of some reticular cells (Akai and Sato, 1979). Some of the authors recognize only the three basic classes and the recent electron microscopic studies have thrown much light on the various types of haemocytes.…”

Section: Introductionmentioning

confidence: 70%

An electron microscopic study of normal haemocytes of Poecilocerus pictus (Fab.) and their response to injected yeast cells

Sharma

Tikku

Saxena

1986

Int. J. Trop. Insect Sci.

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Five types of haemocytes were identified in Poecilocerus pictus (Fab.) and their ultrastructural details and differential counts were studied. The types identified are the prohaemocyte, plasmatocyte, granular haemocyte, oenocytoid and coagulocyte. In response to the injection of yeast cells into the haemocoel, changes in haemocyte morphology and differential counts occurred. The predominant cell involved in phagocytosis is the plasmatocyte, followed by granular haemocyte. Resume-Clinqueespeces des hemocites ont ete identifeiee en Poecilocerus pictus (Fab.) et leur details ultrastructural et comptes differentielle ont ete etudies. Les especes identifiees sont prohemocite, plasmatocite, granulocite, oenocitoid et coagulocite. Par suite d'injection de suspension cellulaire de la levure dans le haemocoel, la morphologie des ces celluss a ete modifiees et les conptes differentielle ont ete variees. Le role predominant est joue par plasmatocites suivre par granulocites dans le processus de phagocitosis pour le mecanism de defence.

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Section: Introductionmentioning

confidence: 70%

An electron microscopic study of normal haemocytes of Poecilocerus pictus (Fab.) and their response to injected yeast cells

Sharma

Tikku

Saxena

1986

Int. J. Trop. Insect Sci.

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“…Together, these studies indicate a commonality in cellular defense reaction in the Acrididae. The rebound in hemocyte number at 1 h postinjection could be due to (1) sedentary cells reentering the hemolymph (Gupta, 1985); or (2) wound healing (Akai & Sato, 1979). Gotz & Boman (1985) and Hou & Chang (1985) observed insufficient encapsulation of B. bassiana in the hemocoel of a dipteran (Chironomus riparius) and a lepidopteran (B. mori) species respectively.…”

Section: Resultsmentioning

confidence: 99%

Hemocytic defense response to the entomopathogenic fungus Beauveria bassiana in the migratory grasshopper Melanoplus sanguinipes

Bidochka

Khachatourians

1987

Entomologia Exp Applicata

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Hemocytic defense response of the migratory grasshopper, Melanoplus sanguinipes (Fab.), to conidia of the white muscardine fungus, Beauveria bassiana (Bals.) Vuill., was studied using phase‐contrast photomicroscopy, hemocyte counting and hemocyte aggregation or ‘nodule formation’. Grasshoppers were injected with an aqueous suspension of conidia. Adherence of B. bassiana conidia to granulocytes occurred within 10 min and the conidia were encapsulated by these hemocytes 6 h postinjection. Hemocytopenia was accompanied by nodule formation after injection of B. bassiana conidia into grasshoppers. The conidia germinated within the nodules and grew into hyphal forms within the hemolymph 12 to 24 h postinjection. We conclude that B. bassiana competes well with nodule formation by hemocytes of M. sanguinipes and often escapes complete encapsulation. Résumé Mécanisme de défense de l'hémocyte de la sauterelle migratrice Melanoplus sanguinipes envers le champignon pathogène Beauveria bassiana Le mécanisme de défense du hémocyte de la sauterelle migratrice Melanoplus sanguinipes envers le champignon pathogène Beauveria bassiana a été etudié a l'aide du photomicroscope a contrase de phase, par dénombrement des hemocytes, ainsi que des nodules formées par l'agrégation des hémocytes. L'adhérence des conidies de B. bassiana aux hemocytes a été observée 10 min après l'injection et leur encapsulement après 1 h. Une baisse des taux d'hémocytes a fait suite a la formation de nodules apres l'injection de conidies dans les sauterelles. Après le déclin initial du taux des hémocytes, une hausse s'est produite dans les sauterelles auquelles on a injecté 106 conidies. Les conidies ont germé dans les nodules et la croissance du mycélium s'est produite dans l'hémolymphe 24 h après avoir injecté. Cette étude a revelé que M. sanguinipes peut exercer temporairement un mécanisme de défense cellulaire contre des conidies fongiques a une concentration de 106 conidies.

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“…These materials cause the deformation of plasmatocytes to assist formation of capsules consisting of multiple layers around the unwanted large substances (Ribeiro and Brehélin, 2006). Other functions of granulocytes, such as haemolymph clotting, wound healing and melanisation were reported in the greater wax moth Galleria mellonella , the migratory locust L. migratoria and mosquitoes (Akai and Sato, 1979; Dushay, 2009; Hillyer and Strand, 2014).…”

Section: Discussionmentioning

confidence: 86%

“…Finally, spherulocytes, which may be a source of cuticular components, are irregularly shaped cells packed with large inclusions (spherules). Rarer insect haemocytes, which are only occasionally observed, include cystocytes, adipohemocytes, vermicytes and megakaryocytes (Akai and Sato, 1979; Brehélin et al, 1978; Gillespie et al, 2000; Gupta, 1979; Jones, 1977; Ren et al, 2014; Ribeiro and Brehélin, 2006). Cystocytes (or coagulocytes) are oval or fusiform cells usually lysed or degranulated in vitro , which differ from granulocytes under the periodic acid-Schiff reaction.…”

Section: Introductionmentioning

confidence: 99%

Cellular response to bacterial infection in the grasshopper Oxya chinensis

Zhang

2019

Biology Open

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Oxya chinensis is one of the most widespread grasshopper species found in China and one of the most common pests against rice. In view of the importance of haemocytes in insect immunity in general, and the lack of information on the haemocytes of O. chinensis, we examined the haemocytes of this species in detail. We challenged the cellular response of this grasshopper with the bacteria Escherichia coli, Staphylococcus aureus, and Bacillus subtilis. Haemocyte morphology was observed using light, scanning electron and transmission electron microscopy, which revealed distinct morphological varieties of haemocytes. Granulocytes and plasmatocytes responded to the bacterial challenge by phagocytosis. Histochemical staining indicated the presence of acid phosphatase in plasmatocytes and granulocytes. We also observed non-phagocytic prohemocytes and vermicytes, but their functions in the circulation are unclear. Insect haemocytes play a crucial role in cellular immunity, and further research is needed for a comprehensive understanding.

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Surface and internal ultrastructure of hemocytes of some insects

Cited by 14 publications

References 0 publications

An electron microscopic study of normal haemocytes of Poecilocerus pictus (Fab.) and their response to injected yeast cells

An electron microscopic study of normal haemocytes of Poecilocerus pictus (Fab.) and their response to injected yeast cells

Hemocytic defense response to the entomopathogenic fungus Beauveria bassiana in the migratory grasshopper Melanoplus sanguinipes

Cellular response to bacterial infection in the grasshopper Oxya chinensis

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