Surface Antigen Changes during Normal Neutrophilic Development: A Critical Review

Invasive breast cancer reprograms early myeloid differentiation in the bone marrow to generate immunosuppressive neutrophils

“…Of note, T-cell proliferation is highly suppressed by a subset of CD15 + /CD16 low neutrophils that expand in the blood of terminal cancer patients (43). Similar to Ly6G in mice, CD16 expression increases on human neutrophils with maturation (44) and is decreased on newly produced, but not mobilized, mature neutrophils in healthy volunteers after G-CSF stimulation (45). It is intriguing to speculate that G-CSF could be driving the production of CD16 low immunosuppressive neutrophils in cancer patients and that targeting G-CSF in these patients in combination with an immunotherapy that targets T-cell activation may increase antitumor efficacy and possibly even NK function (46).…”

Quantitative analysis of the expression of glycosylphosphatidylinositol‐anchored proteins during the maturation of different hematopoietic cell compartments of normal bone marrow

“…According to our results, the expression of both markers is up-regulated in most cell lineages, except on erythroid cells, for which, the lowest expression is observed on PB erythrocytes. In turn, other GPI-AP related to the immune response such as CD14 (LPS receptor) (22) and CD16 (Fcg receptor III) (10,23) appear at the later stages of the monocytic and neutrophil maturation, respectively.…”

“…at the myelocyte and band maturation stages, respectively (5,6); in turn CD66b and CD16 would be expressed at earlier stages of maturation, the highest expression of CD16 being observed on bands and mature neutrophils (7)(8)(9)(10)(11). CD14 expression would be restricted to the latest stages of the maturation of normal monocytic cells (12).…”

The surface molecule signature of primary human acute myeloid leukemia (AML) cells is highly associated with NPM1 mutation status

“…All patients were examined with regard to the expression of CD11c (expressed on monocytes), CD13 (monocytes, neutrophils), CD14 (mainly expressed on monocytes, at lower levels on neutrophils), CD15 (mainly neutrophils, also monocytes), CD33 (appears on myelomonocytic precursors after CD34), CD34 (immature hematopoietic cells), CD45 (leukocytes) and HLA-DR. 4 The French-American-British (FAB) classification was used for morphological evaluation of the differentiation status, 5 and analysis for FLT3 and NPM-1 mutations was performed as previously described. 6 Bioinformatical analyses were performed using the J-Express 2009 analysis suite (MolMine AS, Bergen, Norway) 7 and hierarchical clustering was performed with Pearson's correlation as distance measure and complete weighted linkage.…”