Surface antigens of rat liver epithelial cells grown in medium containing foetal bovine serum

Embleton, M. J.; Iype, P T

doi:10.1038/bjc.1978.229

Cited by 10 publications

(3 citation statements)

References 10 publications

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“…Additionally, antigenic determinants were expressed on transplanted respiratory-tract carcinoma induced in vivo with DMBA and not adapted to cell culture. Thus, growth in tissue culture is not required for antigenic expression as has been demonstrated for other cell culture systems (Thorpe et al, 1977;Peterson et al, 1981) or due to media components non-specifically absorbed to the cell surface (Embleton and Iype, 1978).…”

Section: Discussionmentioning

confidence: 97%

Monoclonal antibodies directed against rat tracheal epithelial cells transformed in vitro

Braslawsky¹,

Kennel²,

Hand³

et al. 1984

Intl Journal of Cancer

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Cloned hybridoma cell lines were obtained by fusion of murine myeloma cells with spleen lymphocytes of either F344 rats or BALB/c mice immunized against the malignant F344 tracheal cell line, 2-10-1. Monoclonal antibodies were selected for their ability to bind to the immunizing cell line and not to normal tracheal epithelial cells. Results from quantitative binding assays indicated that such monoclonal antibodies recognized six epitopes. Each epitope was detected on four other malignant tracheal epithelial cell lines, and was also expressed during early preneoplastic cell passages (i.e. before the cells acquired the ability to produce carcinomas in vivo). Quantitative differences in epitope expression between non-tumorigenic and tumorigenic passages of individual cell lines could not be detected for five of the epitope groups. However, two monoclonal antibodies, recognizing the same epitope, did show quantitative binding differences between non-tumorigenic and tumorigenic cell passages on three of the five cell lines tested. Our results show that carcinogen-altered tracheal cell populations can be distinguished from non-altered cells (by our current assay methods) by use of monoclonal antibodies. The suggest that the antigen expression is an early event associated with the transformation of rat tracheal epithelial cells in culture.

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Section: Discussionmentioning

confidence: 97%

Monoclonal antibodies directed against rat tracheal epithelial cells transformed in vitro

Braslawsky¹,

Kennel²,

Hand³

et al. 1984

Intl Journal of Cancer

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“…F-344 respiratory tract cell lines do not, however, exhibit significant reverse transcriptase activity, nor is there antibody in immune sera for p30 or gp70 molecules of murine leukemia virus (Braslawsky et al, 1981;Jamasbi and Nettesheim, 1977). Immunization with cells propagated in v i m does pose the possibility of antibody responses against tissue culture components (Embleton and Iype, 1978). For this reason, all immunizations were done using cultures propagated in syngeneic rat serum.…”

Section: Discussionmentioning

confidence: 99%

Detection of tumor antigens by syngeneic antiserum on in vitro, transformed tracheal epithelial cell line 2‐10‐1

Braslawsky

Kennel

Steele

et al. 1982

Intl Journal of Cancer

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Sera obtained from rats showing transplantation immunity to syngeneic, malignant epithelial cell line, 2-10-1, were used to identify antigens associated with transformation in vitro. The 2-10-1 cell line was derived from exposure of tracheal explants, in vitro, to the carcinogen MNNG. Tumorigenic passages of the cell line were shown to have common antigenic determinants, shared by independently transformed malignant tracheal epithelial cell lines, as well as antigenic determinants that appear to be limited to the immunizing cell line, 2-10-1. Care was taken to ensure that antibodies were not produced against viral antigens or non-specifically absorbed serum components. Early passages of the 2-10-1 cell line were not tumorigenic in athymic BALB/c (nu/nu) mice, but became malignant with serial passage in vitro. Antigenic specificities recognized by 2-10-1 immune sera were also present on early-passage 2-10-1 cells that had not as yet acquired the malignant phenotype. Such antigen expression must be an early event in neoplastic development.

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“…Tumorigenicity of the MNNG-induced tracheal cell lines was determined by inoculation at various passages of 106 cells into immunosuppressed F-334 rats or BALB/c (nu/nu) mice (Table I) culture can adsorb antigenic determinants from the serum in culture medium (Embleton & Iype, 1978) In order to test more fully for the presence of antigen-bearing cells among normal or transformed epithelial cells (Engers & MacDonald, 1976) immune spleen cells were co-cultivated for 6 days in vitro with primary tracheal-outgrowth cultures or neoplastic epithelial cell lines before 20h microcytotoxicity testing. As shown in Table IV, significant reactivity for the immunizing target cell was obtained when immune spleen cells were co-cultivated with either of the neoplastic cell lines.…”

Section: Development Of Tumorigenicity Of Tracheal Cell Linesmentioning

confidence: 99%

Syngeneic immune response to rat tracheal epithelial cells transformed in vitro by N-methyl-N-nitro-N-nitrosoguanidine

Braslawsky¹,

Steele²,

Kennel³

et al. 1981

Br J Cancer

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Two cell lines (2-10-1 and 8-10-2) derived by exposure to primary tracheal explants to MNNG in vitro were not tumorigenic in syngeneic F-344 rats or athymic BALB/c (nu/nu) mice at early passage, but became tumorigenic at late passage. These cell lines are therefore suited to study the expression of neoantigens during neoplastic development. Transplantation resistance to late-passage, tumorigenic cells was indicated in syngeneic rats using an immunization protocol of repeated cell inoculation and tumour ablation. Spleen cells from such animals were reactive in 20h microcytotoxicity assays against neoplastic cell lines, but unreactive to normal tracheal epithelial cells. Similarly, immune spleen cells co-cultivated in vitro for 6 days with irradiated neoplastic cell lines before assay for microcytotoxicity were strongly reactive, whereas co-cultivation with normal epithelial cells did not stimulate reactivity. Antibody to these neoplastic cell lines was demonstrated in sera of tumour-resistant rats by an indirect radiolabelled-antibody binding test and by indirect immunofluorescence. There was no significant binding to normal tracheal epithelial cell outgrowths. Images Fig. 1 Fig. 2

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Surface antigens of rat liver epithelial cells grown in medium containing foetal bovine serum

Cited by 10 publications

References 10 publications

Monoclonal antibodies directed against rat tracheal epithelial cells transformed in vitro

Monoclonal antibodies directed against rat tracheal epithelial cells transformed in vitro

Detection of tumor antigens by syngeneic antiserum on in vitro, transformed tracheal epithelial cell line 2‐10‐1

Syngeneic immune response to rat tracheal epithelial cells transformed in vitro by N-methyl-N-nitro-N-nitrosoguanidine

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