Surface‐assisted laser desorption/ionization mass spectrometry imaging: A review

Müller, Wendy; Verdin, Alexandre; Pauw, Edwin De; Malherbe, Cédric; Eppe, Gauthier

doi:10.1002/mas.21670

Cited by 69 publications

(64 citation statements)

References 284 publications

(766 reference statements)

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“…While SALDI MSI applications are an emerging field of research, little is known about the underlying mechanisms of desorption and ionization [ 37 ]. For SALDI, employing a variety of mostly inorganic materials with different shapes, sizes, or nanostructures, elucidation of these mechanisms is an ongoing matter of debate.…”

Section: Discussionmentioning

confidence: 99%

Matrix-Free High-Resolution Atmospheric-Pressure SALDI Mass Spectrometry Imaging of Biological Samples Using Nanostructured DIUTHAME Membranes

2021

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Applications of mass spectrometry imaging (MSI), especially matrix-assisted laser desorption/ionization (MALDI) in the life sciences are becoming increasingly focused on single cell analysis. With the latest instrumental developments, pixel sizes in the micrometer range can be obtained, leading to challenges in matrix application, where imperfections or inhomogeneities in the matrix layer can lead to misinterpretation of MS images. Thereby, the application of premanufactured, homogeneous ionization-assisting devices is a promising approach. Tissue sections were investigated using a matrix-free imaging technique (Desorption Ionization Using Through-Hole Alumina Membrane, DIUTHAME) based on premanufactured nanostructured membranes to be deposited on top of a tissue section, in comparison to the spray-coating of an organic matrix in a MALDI MSI approach. Atmospheric pressure MALDI MSI ion sources were coupled to orbital trapping mass spectrometers. MS signals obtained by the different ionization techniques were annotated using accurate-mass-based database research. Compared to MALDI MSI, DIUTHAME MS images captivated with higher signal homogeneities, higher contrast and reduced background signals, while signal intensities were reduced by about one order of magnitude, independent of analyte class. DIUTHAME membranes, being applicable only on tissue sections thicker than 50 µm, were successfully used for mammal, insect and plant tissue with a high lateral resolution down to 5 µm.

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Section: Discussionmentioning

confidence: 99%

Matrix-Free High-Resolution Atmospheric-Pressure SALDI Mass Spectrometry Imaging of Biological Samples Using Nanostructured DIUTHAME Membranes

2021

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“…While the SALDI-MS is not as deeply studied, several thermal and non-thermal desorption and ionization processes are discussed, and a recent review gives an excellent summary. [31]…”

Section: Utilizing Classic Matrices For Lmw Compound Analytics 21 Tmentioning

confidence: 99%

“…Their utilization is usually classified as surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS), and several excellent publications give further details. [31,[73][74][75][76] Therefore, we will only highlight some selected examples: Dong et al [77] used graphene for the analyses of amino acids, anticancer drugs, nucleosides, and steroids. Due to the high surface area, graphene can efficiently capture analyte molecules.…”

Section: Binary Hybrid and Nanomaterial-based Matricesmentioning

confidence: 99%

“…As these matrices will generally not desorb, the ionization process is likely more comparable to surface‐assisted laser desorption/ionization mass spectrometry (SALDI‐MS). While the SALDI‐MS is not as deeply studied, several thermal and non‐thermal desorption and ionization processes are discussed, and a recent review gives an excellent summary [31] …”

Section: Utilizing Classic Matrices For Lmw Compound Analyticsmentioning

confidence: 99%

“…Inorganic carbon‐, silicon‐ or metal‐based materials, such as graphene and metal‐organic frameworks (MOFs), have a high surface area and are vacuum stable. Their utilization is usually classified as surface‐assisted laser desorption/ionization mass spectrometry (SALDI‐MS), and several excellent publications give further details [31,73–76] . Therefore, we will only highlight some selected examples: Dong et al [77] .…”

Section: Rational Design Of Newly Developed Matrix Systemsmentioning

confidence: 99%

See 2 more Smart Citations

MALDI Matrices for the Analysis of Low Molecular Weight Compounds: Rational Design, Challenges and Perspectives

Qiao

Lissel

2021

Chemistry An Asian Journal

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The analysis of low molecular weight (LMW) compounds is of great interest to detect small pharmaceutical drugs rapidly and sensitively, or to trace and understand metabolic pathways. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) plays a central role in the analysis of high molecular weight (bio)molecules. However, its application for LMW compounds is restricted by spectral interferences in the low m/z region, which are produced by conventional organic matrices. Several strategies regarding sample preparation have been investigated to overcome this problem. A different rationale is centred on developing new matrices which not only meet the fundamental requirements of good absorption and high ionization efficiency, but are also vacuum stable and "MALDI silent", i. e., do not give matrix-related signals in the LMW area. This review gives an overview on the rational design strategies used to develop matrix systems for the analysis of LMW compounds, focusing on (i) the modification of well-known matrices, (ii) the search for high molecular weight matrices, (iii) the development of binary, hybrid and nanomaterial-based matrices, (iv) the advance of reactive matrices and (v) the progress made regarding matrices for negative or dual polarity mode.

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Thermally Engineered Ferric Yolk‐Shells for Vaginal Secretion Based Cervical Cancer Screening

Wu,

Wei,

Shi

et al. 2024

Adv Funct Materials

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Engineering of materials is crucial for the advanced detection of bio‐molecules, particularly in laser desorption/ionization mass spectrometry (LDI‐MS). However, effective screening with enhanced LDI performance remains challenging due to the lack of engineered substrate materials. Herein, thermally engineered ferric yolk‐shells are designed for enhanced metabolic screening of cervical cancer (CC) using vaginal secretion. A facile thermal engineering strategy is developed to prepare amorphous ferric yolk‐shells with integrated properties. Notably, the amorphous mesoporous yolk‐shells offer signal amplification up to orders of magnitude in LDI‐MS for standard metabolites and diverse bio‐samples. High‐performance CC screening (including identification of human papillomavirus (HPV) infection, high‐grade squamous intraepithelial lesion (HSIL), and CC) is achieved, with the areas under the curve of 0.842–0.963. Finally, a metabolic panel for CC screening, covering the stages of HPV, HSIL, and CC, is established. This work elucidates the LDI‐MS mechanism for enhanced metabolic detection and highlights potential applications in cancer screening.

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Surface‐assisted laser desorption/ionization mass spectrometry imaging: A review

Cited by 69 publications

References 284 publications

Matrix-Free High-Resolution Atmospheric-Pressure SALDI Mass Spectrometry Imaging of Biological Samples Using Nanostructured DIUTHAME Membranes

Matrix-Free High-Resolution Atmospheric-Pressure SALDI Mass Spectrometry Imaging of Biological Samples Using Nanostructured DIUTHAME Membranes

MALDI Matrices for the Analysis of Low Molecular Weight Compounds: Rational Design, Challenges and Perspectives

Thermally Engineered Ferric Yolk‐Shells for Vaginal Secretion Based Cervical Cancer Screening

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