Surface biofunctionalization of three-dimensional porous poly(lactic acid) scaffold using chitosan/OGP coating for bone tissue engineering

Zeng, Sen; Ye, Jianhua; Cui, Zhixiang; Si, Junhui; Wang, Qianting; Peng, Kaiping; Chen, Wenzhe

doi:10.1016/j.msec.2017.03.220

Cited by 43 publications

(20 citation statements)

References 28 publications

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“…5), which was consistent with the previous report in which both the PDA coating and OGP improved the biocompatibility of polymeric scaffolds and thus facilitated cell adhesion and growth. 55,56 At day 7, the cells on the PLLA-PDA-OGP scaffolds are highly fused as a multilayer structure (Fig. 5), and this result also implied that the immobilized OGP further improved the cell growth and proliferation.…”

Section: Cell Adhesion and Proliferationmentioning

confidence: 87%

Polydopamine-modified poly(l-lactic acid) nanofiber scaffolds immobilized with an osteogenic growth peptide for bone tissue regeneration

Liu

et al. 2019

RSC Adv.

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Section: Cell Adhesion and Proliferationmentioning

confidence: 87%

Polydopamine-modified poly(l-lactic acid) nanofiber scaffolds immobilized with an osteogenic growth peptide for bone tissue regeneration

Liu

et al. 2019

RSC Adv.

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“…Most of the synthetic polymeric NF including PLA, PLGA and PCL do not have necessary surface chemistry to conjugate functional proteins or peptides due to high hydrophobicity 9 . Improved hydrophilicity along with the presence of particular functional groups on the surface of electrospun NF plays an important role in cell adhesion, proliferation and migration 10 , 11 . Various techniques such as pulsed laser deposition, ion beam deposition, covalent immobilization, photochemical modification, and plasma treatment have been used to modify the surface chemistry to improve hydrophilicity and introduce functional groups that can serve as biological cues 12 – 16 .…”

Section: Introductionmentioning

confidence: 99%

Aspartic and Glutamic Acid Templated Peptides Conjugation on Plasma Modified Nanofibers for Osteogenic Differentiation of Human Mesenchymal Stem Cells: A Comparative Study

Onak

Şen

Horzum

et al. 2018

Sci Rep

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Optimization of nanofiber (NF) surface properties is critical to achieve an adequate cellular response. Here, the impact of conjugation of biomimetic aspartic acid (ASP) and glutamic acid (GLU) templated peptides with poly(lactic-co-glycolic acid) (PLGA) electrospun NF on osteogenic differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs) was evaluated. Cold atmospheric plasma (CAP) was used to functionalize the NF surface and thus to mediate the conjugation. The influence of the CAP treatment following with peptide conjugation to the NF surface was assessed using water contact angle measurements, Fourier-Transform Infrared Spectroscopy (FTIR) and X-ray Photoelectron Spectroscopy (XPS). The effect of CAP treatment on morphology of NF was also checked using Scanning Electron Microscopy (SEM). Both the hydrophilicity of NF and the number of the carboxyl (-COOH) groups on the surface increased with respect to CAP treatment. Results demonstrated that CAP treatment significantly enhanced peptide conjugation on the surface of NF. Osteogenic differentiation results indicated that conjugating of biomimetic ASP templated peptides sharply increased alkaline phosphatase (ALP) activity, calcium content, and expression of key osteogenic markers of collagen type I (Col-I), osteocalcin (OC), and osteopontin (OP) compared to GLU conjugated (GLU-pNF) and CAP treated NF (pNF). It was further depicted that ASP sequences are the major fragments that influence the mineralization and osteogenic differentiation in non-collagenous proteins of bone extracellular matrix.

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“…Due to almost all of natural polymers coated onto the surface of synthetic polymer matrix, this coating will be an adequate site for the adhesion and further proliferation of cells. However, it is often difficult to achieve a permanence of the modified properties because the coating is likely to be released once in contact with body fluids . Therefore, simpler and easier surface modification methods are required to introduce the desired bioactivity into scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Surface Modification of Electrospun TPU Nanofiber Scaffold with CNF Particles by Ultrasound‐Assisted Technique for Tissue Engineering

Cui

et al. 2017

Macro Materials & Eng

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A straightforward, fast, and versatile technique is developed to fabricate nanofibrous scaffold with excellent hydrophilicity, mechanical properties, and biocompatibility for tissue engineering. The thermoplastic polyurethane (TPU) nanofiber is fabricated by utilizing electrospinning, and then its surface is modified through simply immersing it into cellulose nanofibrils (CNF) dispersion and subjecting to ultrasonication. The results show that the CNF particles are successfully absorbed on the surface of TPU nanofiber. By introducing CNF particles on the surface of TPU nanofiber, the hydrophilicity, mechanical properties of fabricated CNF‐absorbed TPU scaffold are significantly increased. Additionally, the adhesion and proliferation of human umbilical vein endothelial cells cultured on CNF‐absorbed TPU scaffold are prominently enhanced in comparison with those of cultured on TPU scaffold. These findings suggest that the ultrasound‐assisted technique opens up a new way to simply and effectively modify the surface of various scaffolds and the modified scaffold could be shown a great potential in tissue engineering.

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Surface biofunctionalization of three-dimensional porous poly(lactic acid) scaffold using chitosan/OGP coating for bone tissue engineering

Cited by 43 publications

References 28 publications

Polydopamine-modified poly(l-lactic acid) nanofiber scaffolds immobilized with an osteogenic growth peptide for bone tissue regeneration

Polydopamine-modified poly(l-lactic acid) nanofiber scaffolds immobilized with an osteogenic growth peptide for bone tissue regeneration

Aspartic and Glutamic Acid Templated Peptides Conjugation on Plasma Modified Nanofibers for Osteogenic Differentiation of Human Mesenchymal Stem Cells: A Comparative Study

Surface Modification of Electrospun TPU Nanofiber Scaffold with CNF Particles by Ultrasound‐Assisted Technique for Tissue Engineering

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