Surface cells of the ovary and pelvic peritoneum: A histochemical and ultrastructure comparison

Blaustein, Ancel; Lee, Hyun

doi:10.1016/0090-8258(79)90005-2

Cited by 59 publications

(22 citation statements)

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“…17 Thus, care should be taken when cytologic brushings are being performed so as to avoid contamination with underlying ovarian tissues, especially extraovarian mesothelial cells, with which HOSE cells share a number of morphologic characteristics. 18 The findings of the current study indicate that the HOSE is not morphogenetically quiescent, in agreement with previous histologic, immunohistochemical, and ultrastructural studies. 2,3,19 These studies have demonstrated that the HOSE undergoes a series of events during life, including the following: 1) a variable modulation from a monostratified and columnar to a pseudostratified and low-columnar morphology; 2) the formation of nests, invaginations or crypts, and inclusion cysts; 3) the development of papillary processes of various sizes and complexities; 4) a metaplastic tendency toward mü llerian phenotypes, including ciliated or tubal, endometrioid, and endocervical or mucinous; and 5) discrete cytologic alterations reflecting function, cell cycle events, reproductive status, and aging.…”

Section: Discussionsupporting

confidence: 92%

Cytology of human ovarian surface epithelial brushings

Nicosia¹,

Wilbanks²,

Saunders³

et al. 2003

Cancer

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Section: Discussionsupporting

confidence: 92%

Cytology of human ovarian surface epithelial brushings

Nicosia¹,

Wilbanks²,

Saunders³

et al. 2003

Cancer

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“…Androgens can be postulated to play an important role because of their relatively high levels in the ovary (Risch, 1998). Androstenedione, which is produced by the ovary and the adrenal gland, is a relatively weak androgen but the OSE has been shown to express the enzyme 17b-hydroxysteroid dehydrogenase (Blaustein and Lee, 1979) which can convert androstenedione to testosterone, a much more potent androgen. Androstenedione and testosterone are both found in the developing ovarian follicle, in concentrations exceeding those of oestrogens (McNatty et al, 1979;Eden et al, 1990).…”

mentioning

confidence: 99%

The human ovarian surface epithelium is an androgen responsive tissue

2002

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The pathogenesis of epithelial ovarian cancer remains unclear. From epidemiological studies raised levels of androgens have been implicated to increase the risk of developing the disease. The purpose of this study was to determine the responses of normal human ovarian surface epithelium to androgens. We have established primary cultures of human ovarian surface epithelium from patients undergoing oophorectomy for benign disease. Total RNA was isolated from these cultures and expression of mRNA encoding for the androgen receptor was demonstrated using reverse transcriptase polymerase chain reaction. The presence of androgen receptor in sections of normal ovary was also investigated using an antibody against androgen receptor. The effects of androgens on DNA synthesis and cell death were determined. Eight out of eight (100%) cultures expressed mRNA encoding the androgen receptor. The presence of androgen receptor in ovarian surface epithelium of sections of normal ovaries was demonstrated in all sections. Mibolerone, a synthetic androgen, caused a significant stimulation of DNA synthesis in 5 out of 9 (55%) cultures when used at a concentration of 1 nM. Mibolerone also caused a significant decrease in cell death in 2 out of 5 (40%) cultures tested. We have demonstrated that the ovarian surface epithelium is an androgen responsive tissue and that androgens can cause an increase in proliferation and a decrease in cell death. These findings have important implications for the pathophysiology of ovarian carcinogenesis.

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“…However, little attention has been paid to the significance of changes in the morphology of the other part of surface epithelium. From earlier electron microscopic observations, it was suggested that the surface epithelial cells had the functional activity of endocytosis, absorption, transport (1,12,24) and secretion (6,18). Therefore, it can be postulated that these functional activities of the surface epithelial cells may vary, being correlated with their morphological changes.…”

Section: Discussionmentioning

confidence: 99%

“…In the field of gynecology, it is noteworthy that the majority of ovarian carcinomas originate from ovarian surface epithelium and the frequency is higher than mesothelioma on any other organ (20,22). Ultrastructural observations on other mesothelium such as pelvic peritoneum, mesentery and parietal and viceral peritoneum also have been reported (4)(5)(6)21). Among these mesothelium, the variability or heterogeniety in the morphology of the mesothelial cells in different areas of the body were noted (4,5).…”

mentioning

confidence: 94%

“…In par-459 ticular, it is well-known the tt the dynamic morphological changes occur in the ovarian surface epithelium covering the preovulatory follicles during the events leading to ovulation (14,16). Histochemically, the ovarian surface epithelial cells are positive for 17 j3-hydroxysteroid dehydrogenase and for alcian blue indicating presence of sulfated mucopolysaccharides, while peritoneal mesothelial cells are negative (6). In the field of gynecology, it is noteworthy that the majority of ovarian carcinomas originate from ovarian surface epithelium and the frequency is higher than mesothelioma on any other organ (20,22).…”

mentioning

confidence: 99%

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Variations in the morphology and Ca2+-ATPase activity of the ovarian surface epithelium in rats.

Araki

Takashima

1989

Acta Histochem. Cytochem

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The ultrastructure and cytochemical localization of Cat+-activated adenosine triphosphatase (Ca2+-ATPase) activity of the ovarian surface epithelium in immature rats and in adult rats during the estrous cycle and pregnancy were investigated at both light and electron microscopical levels. In the adult rat ovaries at all stages of the estrous cycle, the intensity of Ca2+-ATPase activity and ultrastructure of the surface epithelium were considerably varied in the different regions on the same ovary. The variations in the morphology and Ca2+-ATPase activity likely provide evidence for the differences in the functional activity of the surface epithelium, which are related to the topographical connection with adjacent structures of ovarian cortex, i.e., corpus luteum, preovulatory Graafian follicles, interstitium containing small follicles. In the adult rat ovaries at all reproductive stages, the cuboidal or columnar surface epithelial cells were found on the interstitium containing small follicles. In some places, the columnar surface epithelial cells, forming crypts of the epithelium, invaginated into the ovarian proper. This type of surface epithelial cell had well-developed microvilli on its apical membrane, and showed intense Ca2+-ATPase activity on its apical and lateral membranes. The squamous surface epithelial cells were found on the surface of corpus luteum and preovulatory Graafian follicles. These cells had a low number of microvilli and less activity of Ca2+-ATPase on the apical membrane. In the immature rat ovaries, the surface epithelial cells were in part squamous, in part cuboidal. Both types of surface epithelial cells had few microvilli. Ca2+-ATPase activity on the apical membrane was considerably less on all surfaces of the ovaries. It seems that the transport function of the surface epithelium in the immature ovary is not active as compared with the mature ovary.These results suggest that the surface epithelium may have an active function of local absorption and/or transepithelial transport between pelvic cavity and ovarian proper, and its functional activity is associated with the reproductive function of the ovary.

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Surface cells of the ovary and pelvic peritoneum: A histochemical and ultrastructure comparison

Cited by 59 publications

References 5 publications

Cytology of human ovarian surface epithelial brushings

Cytology of human ovarian surface epithelial brushings

The human ovarian surface epithelium is an androgen responsive tissue

Variations in the morphology and Ca2+-ATPase activity of the ovarian surface epithelium in rats.

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