Surface characteristics and adhesion of <i>Escherichia coli</i> and <i>Staphylococcus epidermidis</i>

Gilbert, Peter; Evans, David J.; Evans, Elizabeth; Duguid, I.G.; Brown, M. R. W.

doi:10.1111/j.1365-2672.1991.tb04665.x

Cited by 77 publications

(24 citation statements)

References 25 publications

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“…As for attached cells, several studies indicate that irreversible attachment to surfaces takes from 20 min to a maximum of 4 h (Gilbert et al, 1991; Lundén et al, 2000; Vatanyoopaisarn et al, 2000). As for “biofilm” referring to 48-h cells, similarly, the term “biofilm” is used for cells grown on different materials (including stainless steel) just after 24 h (Di Bonaventura et al, 2008; Williams et al, 2011; Corcoran et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Inactivation of Foodborne Bacteria Biofilms by Aqueous and Gaseous Ozone

2018

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In this study, the efficacy of treatments with ozone in water and gaseous ozone against attached cells and microbial biofilms of three foodborne species, Pseudomonas fluorescens, Staphylococcus aureus, and Listeria monocytogenes, was investigated. Biofilms formed on AISI 304 stainless steel coupons from a mixture of three strains (one reference and two wild strains) of each microbial species were subjected to three types of treatment for increasing times: (i) ozonized water (0.5 ppm) by immersion in static condition, (ii) ozonized water under flow conditions, and (iii) gaseous ozone at different concentrations (0.1–20 ppm). The Excel add-in GinaFit tool allowed to estimate the survival curves of attached cells and microbial biofilms, highlighting that, regardless of the treatment, the antimicrobial effect occurred in the first minutes of treatment, while by increasing contact times probably the residual biofilm population acquired greater resistance to ozonation. Treatment with aqueous ozone under static conditions resulted in an estimated viability reduction of 1.61–2.14 Log CFU/cm2 after 20 min, while reduction values were higher (3.26–5.23 Log CFU/cm2) for biofilms treated in dynamic conditions. S. aureus was the most sensitive species to aqueous ozone under dynamic conditions. With regard to the use of gaseous ozone, at low concentrations (up to 0.2 ppm), estimated inactivations of 2.01–2.46 Log CFU/cm2 were obtained after 60 min, while at the highest concentrations a complete inactivation (<10 CFU/cm2) of the biofilms of L. monocytogenes and the reduction of 5.51 and 4.72 Log CFU/cm2 of P. fluorescens and S. aureus respectively after 60 and 20 min were achieved. Considering the results, ozone in water form might be used in daily sanitation protocols at the end of the day or during process downtime, while gaseous ozone might be used for the treatment of confined spaces for longer times (e.g., overnight) and in the absence of personnel, to allow an eco-friendly control of microbial biofilms and consequently reduce the risk of cross-contamination in the food industry.

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Section: Discussionmentioning

confidence: 99%

Inactivation of Foodborne Bacteria Biofilms by Aqueous and Gaseous Ozone

2018

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“…As the hydrophobicity of the cell increases, the level of adhesion also increases [24]. According to Gilbert et al [25], only for hydrophobic microorganisms surface hydrophobicity is correlated to adhesions. Among the isolates, isolates of sour dough SD2, SD3 and SD4 showed hydrophobicity values 52.4, 24 and 73% respectively; snake gourd isolates SG1, SG2 had values 65 and 40% and MC1, 23% hydrophobicity.…”

Section: Hydrophobicity Of Isolatesmentioning

confidence: 96%

Evaluation of the Probiotic Characteristics of Newly Isolated Lactic Acid Bacteria

Girija

Ismail

John

et al. 2008

Appl Biochem Biotechnol

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Lactic acid bacteria were isolated from fermented vegetables, sour dough, milk products, sheep and human excreta. The newly isolated cultures were evaluated for a number of probiotic characteristics like bile salt resistance, salt tolerance in general, survival in low pH, hydrophobicity of the cell surface, resistance to low phenol concentration, antimicrobial activity and susceptibility pattern against vancomycin and erythromycin. The selected cultures were further screened for their ability to produce the nutraceticals such as folic acid and exopolysaccharide (EPS). Two potent isolates, CB2 (from cabbage) and SD2 (from sour dough) were found to produce both extracellular and intracellular folate. One of the isolates from yogurt (MC-1) and the one from whey (W3) produced significant amount of EPS with a maximum production of 8.79 +/- 0.05 g/l by MC-1.

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“…This question is especially justified for hydrophilic microorganisms living on inner or outer human body surfaces, which have mostly hydrophobic character (Gilbert et al, 1991; Feingold, 2007; Linden et al, 2008). …”

Section: Are Hydrophobic Microorganisms More Pathogenic?mentioning

confidence: 99%

How microorganisms use hydrophobicity and what does this mean for human needs?

Krasowska

Sigler

2014

Front. Cell. Infect. Microbiol.

500

307

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Cell surface hydrophobicity (CSH) plays a crucial role in the attachment to, or detachment from the surfaces. The influence of CSH on adhesion of microorganisms to biotic and abiotic surfaces in medicine as well as in bioremediation and fermentation industry has both negative and positive aspects. Hydrophobic microorganisms cause the damage of surfaces by biofilm formation; on the other hand, they can readily accumulate on organic pollutants and decompose them. Hydrophilic microorganisms also play a considerable role in removing organic wastes from the environment because of their high resistance to hydrophobic chemicals. Despite the many studies on the environmental and metabolic factors affecting CSH, the knowledge of this subject is still scanty and is in most cases limited to observing the impact of hydrophobicity on adhesion, aggregation or flocculation. The future of research seems to lie in finding a way to managing the microbial adhesion process, perhaps by steering cell hydrophobicity.

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Surface characteristics and adhesion of Escherichia coli and Staphylococcus epidermidis

Cited by 77 publications

References 25 publications

Inactivation of Foodborne Bacteria Biofilms by Aqueous and Gaseous Ozone

Inactivation of Foodborne Bacteria Biofilms by Aqueous and Gaseous Ozone

Evaluation of the Probiotic Characteristics of Newly Isolated Lactic Acid Bacteria

How microorganisms use hydrophobicity and what does this mean for human needs?

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