Surface Characteristics of Biomaterials Used for Space Maintenance in a Mandibular Defect: A Pilot Animal Study

Nguyen, Charles; Young, Simon; Kretlow, James D.; Mikos, Antonios G.; Wong, Mark

doi:10.1016/j.joms.2010.02.026

Cited by 25 publications

(19 citation statements)

References 43 publications

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“…[4, 8] Strategies to address stabilization and bone regeneration have typically employed a solid and porous composite structure or a solid structure with degradable porogens to induce porosity. [20, 21] In a study by Kempen et al, a PPF intramedullary rod embedded with poly(DL-lactic- co- glycolic acid) (PLGA) microparticles for growth factor delivery was used. [21]…”

Section: 1 Introductionmentioning

confidence: 99%

Biodegradable composite scaffolds incorporating an intramedullary rod and delivering bone morphogenetic protein-2 for stabilization and bone regeneration in segmental long bone defects

et al. 2011

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In this study, a two part bone tissue engineering scaffold was investigated consisting of a solid poly(propylene fumarate) (PPF) intramedullary rod for mechanical support surrounded by a porous PPF sleeve for osseointegration and delivery of poly(DL-lactic-co-glycolic acid) (PLGA) microspheres with adsorbed recombinant human bone morphogenetic protein-2 (rhBMP-2). Scaffolds were implanted into critical size rat segmental femoral defects with internal fixation for 12 weeks. Bone formation was assessed throughout the study via radiography, and following euthanasia via micro-CT and histology. Mechanical stabilization was evaluated further via torsional testing. Experimental implant groups included the PPF rod alone and the rod with a porous PPF sleeve containing PLGA microspheres with 0, 2, or 8 μg of rhBMP-2 adsorbed onto their surface. Results showed that presence of the scaffold increased mechanical stabilization of the defect, as evidenced by the increased torsional stiffness of the femurs by the presence of a rod compared to the empty defect. Although the presence of a rod decreased bone formation, the presence of a sleeve combined with a low or high dose of rhBMP-2 increased the torsional stiffness to 2.06 ± 0.63 N·mm and 1.68 ± 0.56 N·mm, respectively, from 0.56 ± 0.24 N·mm for the rod alone. The results indicate that, while scaffolds may provide structural support to regenerating tissues and increase their mechanical properties, the presence of scaffolds within defects may hinder overall bone formation if they interfere with cellular processes.

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Section: 1 Introductionmentioning

confidence: 99%

Biodegradable composite scaffolds incorporating an intramedullary rod and delivering bone morphogenetic protein-2 for stabilization and bone regeneration in segmental long bone defects

et al. 2011

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“…Recently in the United States, a conglomerate of funding sources combined to form the Armed Forces Institute of Regenerative Medicine (AFIRM), marking a large investment in tissue engineering and biomaterials research with the goal of fostering rapid clinical translation of new technology for the treatment of wounded warriors 80, 81. Within our laboratory, a recent focus toward the goal of increasing applications in which scaffold‐based tissue engineering approaches can be employed has resulted in the development of porous poly(methylmethacrylate) (PMMA) space maintainers to better prepare hostile wound environments for eventual scaffold or construct implantation 82–85. Although these two paths differ in approach and timeline, greater clinical success will only reinforce the potential of biomaterials and tissue engineering, expanding interest and hopefully funding for the development of novel biomaterials that improve upon those in use.…”

Section: Introductionmentioning

confidence: 99%

Silk grafting with methacrylamide: A near‐infrared spectroscopy study

Zoccola

Boschi

Arosio

et al. 2010

J of Applied Polymer Sci

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The grafting of methacrylamide (MAA) onto silk has taken importance not only as weighting process but also as finishing operation to improve the properties of the silk fibers. It was confirmed that the MAA monomer concentration, the initiator amount, and the liquor ratio are the major parameters affecting the yield of the reaction. Varying these parameters, a large number of samples, i.e., silk yarns with different grafting yields and exhaust grafting baths with different residual MAA concentrations, were obtained. Near infrared (NIR) spectroscopy was used for the quantitative determination of MAA in silk yarns and MAA in exhaust baths after grafting. A correlation between silk yarns grafting yield and NIR spectra of MAA grafted yarns was obtained. This correlation shows a coefficient of determination (R 2 ) of 0.96 and a standard error of calibration (SEC) of 8.37 w/w for a mean value (M) of 31.82% w/w. A second correlation between MAA in exhaust grafting baths and NIR spectra was obtained. This correlation shows a R 2 of 0.95 and a SEC of 2.93 g/L for a M of 17.16 g/L. Previous correlations were improved using silk samples cleaned by extraction of sizing agents and detergents which could interfere with MAA grafting and modify absorption spectra of bath solutions.

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“…5 Additionally, a separate study by Nyugen et al using PPF constructs in a similar animal model found that porous PPF resulted in less dehiscence when compared with solid implants. 27 Constructs in this study were designed to degrade and become increasingly porous with time to facilitate soft tissue growth over the implant and enable later ingrowth of regenerated bone. MicroCT analysis of constructs over the 8 week degradation study under accelerated conditions showed that porosity continued to increase from day 1 to week 8.…”

Section: Discussionmentioning

confidence: 99%

Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications

Henslee

Shah

Wong³

et al. 2014

J. Biomed. Mater. Res.

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Space maintainers (SMs) used for craniofacial reconstruction function to preserve the void space created upon bone loss and promote soft tissue healing over the defect. Polymethylmethacrylate-based SMs present several drawbacks including implant exposure, secondary removal surgeries, and potential bacterial contamination during implantation. To address these issues, a novel composite material comprising poly(propylene fumarate) (PPF) with N-vinyl pyrrolidone (NVP) as the crosslinking agent, carboxymethylcellulose (CMC) hydrogel as a porogen, and antibiotic loaded poly(lactic-co-glycolic acid) (PLGA) microparticles as antibiotic carriers and porogen was fabricated. CMC was incorporated at 40 wt % to impart rapid porosity while PLGA microparticles were incorporated at 30 or 40 wt % to release either clindamycin or colistin. This study was designed to examine the effects of PPF:NVP ratio, PLGA wt %, and the drug dose on the mass loss, temporal porosity change and drug release kinetics of the composite construct. Mass loss decreased significantly in constructs containing 3:2 PPF:NVP ratio with 30 wt % PLGA (63.2 ± 0.8%) compared to the 2:3 PPF:NVP ratio (80.3 ± 1.0% and 85.3 ± 1.3% for 30 and 40 wt % PLGA content, respectively) at 8 weeks. In formulations with 3:2 PPF:NVP ratio, incorporation of 40 versus 30 wt % PLGA significantly increased the porosity at 8 weeks under accelerated degradation conditions. Constructs released clindamycin or colistin at concentrations above the minimum inhibitory concentration for target pathogens for 45 and 77 days, respectively. This study demonstrates that the composition of PPF/CMC/PLGA constructs can be modulated to achieve properties suitable for craniofacial degradable space maintenance applications.

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Surface Characteristics of Biomaterials Used for Space Maintenance in a Mandibular Defect: A Pilot Animal Study

Cited by 25 publications

References 43 publications

Biodegradable composite scaffolds incorporating an intramedullary rod and delivering bone morphogenetic protein-2 for stabilization and bone regeneration in segmental long bone defects

Biodegradable composite scaffolds incorporating an intramedullary rod and delivering bone morphogenetic protein-2 for stabilization and bone regeneration in segmental long bone defects

Silk grafting with methacrylamide: A near‐infrared spectroscopy study

Degradable, antibiotic releasing poly(propylene fumarate)-based constructs for craniofacial space maintenance applications

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