Surface charge-controlled electron transfer and catalytic behavior of immobilized cytochrome P450 BM3 inside dendritic mesoporous silica nanoparticles

Harnessing P450 Enzyme for Biotechnology and Synthetic Biology

“…In this work, the PNGFs not only served as the electron transfer medium between electrode and CYP3A4, but also acted as host and provided benign microenvironment for CYP‐catalyzed metabolic reaction due to the unique properties of polydopamine [102] . Very recently, the same group immobilized P450BM3 into the dendritic mesoporous silica nanoparticles with different surface functional groups (OH‐DMSNs and NH 2 ‐DMSNs) by physical adsorption, then coated the hybrids on GC electrodes to form a film with the help of chitosan [103] . The positively charged internal walls of NH 2 ‐DMSNs could orientate the P450 in a suitable position that favored the electron exchange between the reductase domain and the electrodes, and the substrate binding with the active site of enzyme [103] .…”

“…[102] Very recently, the same group immobilized P450BM3 into the dendritic mesoporous silica nanoparticles with different surface functional groups (OH-DMSNs and NH 2 -DMSNs) by physical adsorption, then coated the hybrids on GC electrodes to form a film with the help of chitosan. [103] The positively charged internal walls of NH 2 -DMSNs could orientate the P450 in a suitable position that favored the electron exchange between the reductase domain and the electrodes, and the substrate binding with the active site of enzyme. [103] Although no turnover data was provided, this work provided implications about the importance of protein orientation in the CYP bioreactors.…”

“…[103] The positively charged internal walls of NH 2 -DMSNs could orientate the P450 in a suitable position that favored the electron exchange between the reductase domain and the electrodes, and the substrate binding with the active site of enzyme. [103] Although no turnover data was provided, this work provided implications about the importance of protein orientation in the CYP bioreactors.…”

An Overview of Cytochrome P450 Immobilization Strategies for Drug Metabolism Studies, Biosensing, and Biocatalytic Applications: Challenges and Opportunities

“…As discussed above, oriented immobilization of the enzyme on the electrode can improve heme-electrode electron coupling. , This was demonstrated by immobilizing P450 BM3 on either hydroxylated or aminated dendritic mesoporous silica nanoparticles that were then attached to a carbon glass electrode with film-forming chitosan for bioelectrochemical measurement of testosterone hydroxylation . The catalytic current generated by the aminated system was 1.8 times higher than by the hydroxylated system, suggesting that the positively charged aminated surface oriented enzyme binding, holding the reductase domain proximal to the electrode surface and thereby providing more efficient electron transfer to the heme center.…”

Multifunctionalized Mesostructured Silica Nanoparticles Containing Mn2 Complex for Improved Catalase-Mimicking Activity in Water

“…Among different applications of stellate-like MSN, their employment as a platform in the aim to protect and support catalytically active species and improve their activity has attracted a particular interest. In fact, the open porosity of the dendritic nanoparticles allows the incorporation into the pores of various large species presenting promising catalytic properties, such as enzymes, complexes, and small nanoparticles, while keeping a great diffusion of the active molecules [ 16 , 21 , 24 ]. For instance, small PdO nanoparticles for the catalysis of methane combustion [ 25 ], Pd nanoparticles for the Suzuki coupling reactions [ 26 ], and Ru nanoparticles for the hydrogenolysis of alkanes have been incorporated inside the pores [ 27 ].…”