Surface Charge on Functionalized Latex Spheres in Aqueous Colloidal Suspensions

Schulz, Susanne; Gisler, Thomas; Borkovec, Michal; Sticher, H.

doi:10.1006/jcis.1994.1146

Cited by 47 publications

(50 citation statements)

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“…The wall charge density at t Å 0, s w (0), can also be calculated from DF w extr , by applying Eq. [10] (see Fig. 4).…”

Section: Pore Pcl During the Ionmentioning

confidence: 92%

“…The bead surface can be represented by an isolated flat / (z j 0 1)a j (t, x)}, [2] polystyrene surface, since the Debye length is negligible in relation to the bead diameter at the salt concentrations used where the superscript w refers to the pore wall, z j is the (10).…”

Section: General Assumptions Top Of Both Ion Sensors Is Deposited a Mmentioning

confidence: 99%

“…The measuring signal is the output voltage of the ISFET amplifier while grounding the electrolyte with the Ag/AgCl The term K(t, x) is a scaling factor for the proton membrane DF w extr Å 2RT [10] diffusion coefficient, representing the retardation of proton diffusion due to association with fixed groups. Equation [14] shows that K is proportional to the ratio of the buffer capacitwhere s w Å s w / 8 RT e 0 e r Å 269s w ( mol 0.5 m 01.5 ) , and c e = ies of the wall-fixed groups and pore solution.…”

Section: The Measuring Ion Sensorsmentioning

confidence: 99%

“…All figures show a similar change into Eq. [10]. Then the titration curve is obtained by conin curve slope as a function of pH, indicating a low buffer necting these s w (0) points.…”

Section: Titration Curves Constructedmentioning

confidence: 99%

“…suspension in a small volume. In the first type of experi- [10 ] . From the two s w values obtained, the increase in ments, the apparent wall buffer capacity (mol m 02 ) was wall charge density between pH e Å 5.1 and 6.9 was calobtained directly from the suspension buffer capacity by culated.…”

Section: Comparison With Equilibrium Experimentsmentioning

confidence: 99%

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Constructing a Proton Titration Curve from Ion-Step Measurements, Applied to a Membrane with Adsorbed Protein

Eijkel

Bosch

Olthuis

et al. 1997

Journal of Colloid and Interface Science

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Previously it was shown that the potential transient is A new measuring method is described for obtaining a proton determined by a proton release or uptake in the membrane titration curve. The curve is obtained from a microporous compos-(7). In this paper the theoretical relationships are derived ite membrane, consisting of polystyrene beads in an agarose ma-between the amplitude and the duration of the transients and trix, with lysozyme molecules adsorbed to the bead surface. The the proton titration curve of the membrane at a certain pH.membrane is incorporated into a sensor system by deposition on a Using these relationships, and measuring the potential transilicon chip with a pH-sensitive ion-sensitive field effect transistor sients at a series of pH values, it will be possible to construct (ISFET) located in the middle of a Ag/AgCl electrode. The actual a titration curve and thus to obtain information on adsorbed measurement is performed by creating a stepwise change in the macromolecules. For biosensor purposes, the relationships salt concentration of the bathing electrolyte (the ion step) and measuring the ISFET potential versus the Ag/AgCl electrode. This can also be applied in the inverse direction; i.e., the device potential shows a transient change in the ion step, which indicates response can be predicted from known titrational behavior a transient pH change in the membrane. This procedure is re-of the different components. In this paper the adsorption peated at a series of pH values. Equations are presented to calcu-of the positively charged protein lysozyme is studied as a late the proton titration curve of the membrane from the amplitude model. and duration of the measured transients. Measurements showThe measurements presented can be regarded as an extenqualitative agreement between the curves obtained and equilib-sion of common practice in protein titration, namely, the rium titration experiments on the same system. ᭧ 1997 Academic Press relative positioning of the titration curves at different ionic Key Words: proton titration; ion-sensitive field effect transistor; strengths by using the pH change that results from a change polystyrene bead; lysozyme; concentration step; Ag/AgCl elecin ionic strength (8,9). In this type of experiment, the pH trode.change is measured at only one initial pH value. In the investigations presented the method is extended by measuring at a series of pH values. The extension is made possible

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“…The wall charge density at t Å 0, s w (0), can also be calculated from DF w extr , by applying Eq. [10] (see Fig. 4).…”

Section: Pore Pcl During the Ionmentioning

confidence: 92%

Section: General Assumptions Top Of Both Ion Sensors Is Deposited a Mmentioning

confidence: 99%

Section: The Measuring Ion Sensorsmentioning

confidence: 99%

“…All figures show a similar change into Eq. [10]. Then the titration curve is obtained by conin curve slope as a function of pH, indicating a low buffer necting these s w (0) points.…”

Section: Titration Curves Constructedmentioning

confidence: 99%

Section: Comparison With Equilibrium Experimentsmentioning

confidence: 99%

See 3 more Smart Citations

Constructing a Proton Titration Curve from Ion-Step Measurements, Applied to a Membrane with Adsorbed Protein

Eijkel

Bosch

Olthuis

et al. 1997

Journal of Colloid and Interface Science

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show abstract

Capillary zone electrophoresis for the characterization of latex particles

2001

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Capillary zone electrophoresis (CZE) was applied to the separation of acrylic styrene copolymer emulsion particles. Fast separations could be performed on samples containing chemically identical latex particles of different size, as well as on samples with particles of the same size but differing in chemical composition. The developed method was also used for the analysis of water soluble fractions of urethane dispersions. Additionally, the physical interaction between different particles (e.g., acrylic and urethane particles) could be studied using this method. The separation mechanism is based on the zeta potential of the particles and the relaxation effect under the applied analytical conditions.

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pH‐Responsive Nanogels: Synthesis and Physical Properties

Tan

Tam

2012

Hydrogel Micro and Nanoparticles

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Surface Charge on Functionalized Latex Spheres in Aqueous Colloidal Suspensions

Cited by 47 publications

References 0 publications

Constructing a Proton Titration Curve from Ion-Step Measurements, Applied to a Membrane with Adsorbed Protein

Constructing a Proton Titration Curve from Ion-Step Measurements, Applied to a Membrane with Adsorbed Protein

Capillary zone electrophoresis for the characterization of latex particles

pH‐Responsive Nanogels: Synthesis and Physical Properties

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