Surface Chemistry and Microtopography of Parylene C Films Control the Morphology and Microtubule Density of Cardiac Myocytes

Trantidou, Tatiana; Humphrey, Eleanor J.; Poulet, Claire; Gorelik, Julia; Prodromakis, Themis; Terracciano, Cesare

doi:10.1089/ten.tec.2015.0581

Cited by 11 publications

(14 citation statements)

References 33 publications

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“…The PP1 scaffolds, after three days from a mild treatment (RIE), show a contact angle almost constant with a value of 80°, still good for cell adhesion. The substrates with a contact angle of 80° are considered weakly hydrophobic [56] and this value of contact angle can be still considered good for promoting cell adhesion as reported by Trantidou et al in [51].…”

Section: Accepted Manuscriptmentioning

confidence: 96%

“…The contact angle of PDMS1 employed on PP2 scaffolds after three days from a strong plasma treatment (ICP) remains almost constant with a value below 60° that was demonstrated to be good for cell adhesion [51], [52]. This result is advantageous for promoting cell adhesion.…”

Section: Surface Wettabilitymentioning

confidence: 99%

“…material to fabricate PP1 and PP2 scaffolds which have in common the height and the elasticity closer to the heart tissue [51], [30]. In addition, PP2 scaffolds have the advantage to have a hybrid surface with stiff and soft areas.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

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Microstructured hybrid scaffolds for aligning neonatal rat ventricular myocytes

Sanzari

Dinelli²,

Humphrey

et al. 2019

Materials Science and Engineering: C

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In cardiac tissue engineering (TE), in vitro models are essential for the study of healthy and pathological heart tissues in order to understand the underpinning mechanisms. In this scenario, scaffolds are platforms that can realistically mimic the natural architecture of the heart, and they add biorealism to in vitro models. This paper reports a novel and robust technique to fabricate cardiovascular-mimetic scaffolds based on Parylene C and Polydimethylsiloxane (PDMS). Parylene C is employed as a mask material for inducing hybrid and nonhybrid micropatterns to the PDMS layer. Hybrid architectures present striped hydrophobic/hydrophilic surfaces, whereas non-hybrid scaffolds only corrugated topographies. Herein, we demonstrate that wavy features on PDMS can be obtained at the micro-and nanoscale and that PDMS can be integrated into the microfabrication process without changing its intrinsic physical properties. A study of the effects of these scaffolds on the growth of Neonatal Rat Ventricular Myocytes (NRVMs) cultures reveals that cell alignment occurs only for the case of hybrid architectures made of hydrophilic PDMS and hydrophobic Parylene C.

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Section: Accepted Manuscriptmentioning

confidence: 96%

Section: Surface Wettabilitymentioning

confidence: 99%

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Microstructured hybrid scaffolds for aligning neonatal rat ventricular myocytes

Sanzari

Dinelli²,

Humphrey

et al. 2019

Materials Science and Engineering: C

Self Cite

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“…Micro and nanofabrication methods allowed to transfer the information at these scales in a controlled, predefined, and versatile way ( Figure 2 a) [ 26 ]. Optical lithography was used to direct the attachment of cardiac cells cultured on surfaces, having a patterning with 20-μm wide lines, to control the morphology and microtubule density of cardiac myocytes [ 27 ]. Methacrylated tropoelastin (MeTro) coating by means microfluidic method allowed the neonatal rat CMs attachment and beating [ 28 ].…”

Section: Nanopatterning Technologies For Cardiac Tissue Regeneratimentioning

confidence: 99%

Nanoengineering in Cardiac Regeneration: Looking Back and Going Forward

et al. 2020

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To deliver on the promise of cardiac regeneration, an integration process between an emerging field, nanomedicine, and a more consolidated one, tissue engineering, has begun. Our work aims at summarizing some of the most relevant prevailing cases of nanotechnological approaches applied to tissue engineering with a specific interest in cardiac regenerative medicine, as well as delineating some of the most compelling forthcoming orientations. Specifically, this review starts with a brief statement on the relevant clinical need, and then debates how nanotechnology can be combined with tissue engineering in the scope of mimicking a complex tissue like the myocardium and its natural extracellular matrix (ECM). The interaction of relevant stem, precursor, and differentiated cardiac cells with nanoengineered scaffolds is thoroughly presented. Another correspondingly relevant area of experimental study enclosing both nanotechnology and cardiac regeneration, e.g., nanoparticle applications in cardiac tissue engineering, is also discussed.

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“…Optical lithography is also used to direct the attachment and the shape of cells which are grown on surfaces. It has been found that when cardiac cells are cultured on surfaces that have been patterned into having typically 20 m wide lines, cells tend to align and stretch their ECM and their nucleus towards the length of the line [27]. Aligning CMs is crucial for the contraction and propagation along the long axis [28].…”

Section: Optical Lithographymentioning

confidence: 99%