Surface chemistry and polymer film thickness effects on endothelial cell adhesion and proliferation

Abstract

Adherence and growth rates of human aortic endothelial cells (HAEC) on plasma polymerized poly (vinylacetic acid) films were measured as functions of the surface density of -COOH groups and plasma deposited film thickness. Pulsed plasma polymerization was employed to produce films containing 3.6 to 9% -COOH groups, expressed as a percent of total carbon content. Endothelial cells exhibited increased cell adherence and proliferation with increasing -COOH surface densities. Additionally, and unexpectedly, cell growth was also dependent on the film thicknesses, which ranged from 25 to 200 nm. The results indicate that optimization of the functional group surface density and film thickness could produce significant enhancements in initial adhesion and subsequent growth of the HAEC cells.

Biocompatibility and inflammatory response in vitro and in vivo to gelatin-based biomaterials with tailorable elastic properties

“…Here, higher crosslinking of the hydrogel led to higher cell adhesion. This may be explained by the different surface properties of the two hydrogels like the wettability [20] or surface roughness, which change during degradation processes [21]. Additionally, available data imply that also the higher substrate stiffness of G10_LNCO8 supports cell adhesion [22].…”

Biodegradable and Biocompatible Poly(Ethylene Glycol)‐based Hydrogel Films for the Regeneration of Corneal Endothelium

“…Previous studies have shown that carboxylic acid groups play an important role in the attachment and migration of various cell types. [ 53 ] In addition, a balance between hydrophilicity and hydrophobicity is required for optimal cell-surface interactions. [ 54 ] Arima and Iwata reported that surface wettability in addition to the functional groups (e.g., carboxylic acid and amine groups) plays a signifi cant role in the adherence of cells.…”

PCR-ready human DNA extraction from urine samples using magnetic nanoparticles

“…Successful PCR amplification was detected from as small as 50 l of sample (lane 1), while at least 200 l urine was needed to generate enough PCR templates in a similar report [25]. This high extraction performance can be ascribed to the carboxylated surfaces of the nanoparticles, which bind mammalian cells with a high affinity [14,24,25,35,36], especially when used with the binding buffer employed in the present study. The higher surface area:volume ratio and good dispersal properties inherent to the nanoparticles employed in this study conferred an advantage over commonly used micro-sized particles, as evidenced by their superior binding capabilities with mammalian cells.…”