Surface chracteristics of treated glasses for the preparation of glass capillary columns in gas-liquid chromatography

Determination of Fatty Acid Composition of Spore Lipids of the Moss Polytrichum commune by Glass Capillary Column Gas Chromatography

“…The fatty acid methyl esters were analysed by GLC with the aid of a Perkin-Elmer Model F 30 gas chromatograph equipped with a hydrogen fiame detector and a precolumn (Gas Chrom Q 80/100 as support material, 1,5% SE 30), The precolumn was found necessary for quantitative analysis. The capillary columns (35 m x 0,3 mm) were drawn using an apparatus produced by Hupe and Busch, Karlsruhe, G,F,R, Then they were etched with HCL according to Alexander et al (1974) and coated with 0,6% FFAP, using the static procedure introduced by Bouche and Verzele (1968) and modified by Stallberg-Stenhagen (1972), The column temperature was held at 220°C, the temperature of the injector and detector blocks at 250°C, The fiow rate of the Nj carrier gas was 20 ml/min at a pressure of 420 kN x m-^ The split ratio was 15:1, AgN03 fractionation (Karunen 1975), analysis by GLC before and after hydrogenation (Karunen 1972), and the lin/log technique were used for identification purposes. Commercial fatty acid methyl esters were used as internal and/or external standards.…”

Contemporary Chromatography

“…Since the time when problems of glass wettability became widely appreciated, our knowledge of the surface chemistry of glass has been extended significantly. Scanning electron micrograph of inner wall of soft-glass capillary, treated with dry hydrogen chloride at high temperature and coated with stationary phase (29) ization (75), surface corrosion with hydrogen chloride or fluoride (16, 17), and deposition of solid particles (25, 26'). All these processes involve an increase of the surface roughness that results in an easier spreading of organic films.…”

“…Although some of these column treatment methods have been empirically known for some time, more recent surface chemistry studies that involve contact angle measurements (28, 2.9) and electron scanning microscopic observations (29,30) now shed more light into the wettability problems. As an example of surface geometrical modification, Figure 6 demonstrates the growth of crystalline aggregates, following high-temperature treatment with dry hydrogen chloride gas according to Novotny and Tesarik (16,17).…”

“…Figure6. Scanning electron micrograph of inner wall of soft-glass capillary, treated with dry hydrogen chloride at high temperature and coated with stationary phase(29) …”

Quantitative determination of underivatized anti-convulsant drugs by high resolution gas chromatography with support-coated open tubular columns

“…One line followed in research is the etching of glass wall with e.g. gaseous hydrogen chloride [9]. The resulting rough surface, however, usually contains many active sites for adsorption.…”