Surface collagen functionalization of electrospun poly(vinyl alcohol) scaffold for tissue engineering

One Produces Multi: A Drug-free Cardiovascular Stent Functionalized with Tailored Collagen Supports in-situ Healing of Vascular Tissues

“…As shown in Fig. 2F (21). The signal of the COOH peak (the tting peaks at 288.3 eV) was not visualized on the (rhCol III/PDA-PEI) n (n = 0.5, 1) coatings, possibly due to the lower amount and uneven distribution of rhCol III on the surface of PLA substrates (7).…”

Collagen-decorated electrospun scaffolds of unsaturated copolyesters for bone tissue regeneration

“…Despite the satisfactory results related to improvement in cell adhesion and proliferation by changes in the physical surface in other functionalization methods (i.e., physical adsorption 31,34 ), bioconjugation is also able to tune both mechanical and thermal properties of the pre-designed scaffolds, promoting a stable attachment of collagen molecules on the polymer surface. EDC/ NHS bioconjugation method, for instance, was employed by Perez-Nava and coworkers 30 to bioconjugate collagen to an electrospun poly(vinyl alcohol) scaffold structure, where the resultant scaffolds presented significant improvements in mechanical behavior (i.e., Young's modulus, elongation at break and ultimate tensile strength), which would not be achieved through a simple physical coating, for example. Regarding tissue engineering purposes, the conjugation between collagen and polymers has been proven to be more efficient in terms of mechanical strength, prolonged degradation rate, and cell viability than the simple solvent mixture, as stated by Sadeghi-Avalshahr et al 8 In addition, the carbodiimide chemistry employed in our work has been widely studied for biomaterial fabrication to improve physical and mechanical resistance or bioactive behavior under mild conditions.…”

“…This bioconjugation method is non-sensitive to light or humidity, involves chemical reactions at room temperature and pressure, occurs in the absence of organic solvents, has high efficiency and the unreacted reagents can be easily removed. 30,55 For this reason, it can be carried out in a one-pot strategy, bestowing the present work with several advantages in terms of reaction media conditions aligned with green synthesis concepts.…”

“…26 In the latter case, collagen could be combined with several types of materials and can create biocompatible structures with high porosity and adhesion. Materials such as polymers derived from natural monomers, [29][30][31][32] natural ceramics such as hydroxyapatite and tricalcium phosphate, 32,33 or composite materials have been widely employed as main components of scaffolds to further collagen-based surface functionalization. These collagen combinations can be developed via polymeric blends, coatings involving physical adsorption in porous structures, 31,34 or collagen covalently binding through bioconjugation.…”

“…These collagen combinations can be developed via polymeric blends, coatings involving physical adsorption in porous structures, 31,34 or collagen covalently binding through bioconjugation. 30 Bioconjugation appears to be an advantageous strategy to bind collagen to scaffolds due to the high stability of the formed covalent bonds and the more efficient collagen attachment when compared to other functionalization methods. 29 In this context, the present work proposes the design of a novel 3D-assembled scaffold for bone tissue engineering.…”