Surface electrophoresis of ds-DNA across orthogonal pair of surfaces

Ghosh, Arnab; Patra, Tarak K.; Kant, Rishi; Singh, Rajeev; Singh, Jayant K.; Bhattacharya, Shantanu

doi:10.1063/1.3565238

Cited by 14 publications

(4 citation statements)

References 19 publications

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“…The best results were obtained using a glass surface that was previously exposed to an oxidizing environment, which led to a scaling μ ∼ N −0.491 for the EcoRI digest of λ DNA. There are also hints that one can enhance the surface electrophoresis process at the interface existing along the corner of a microchannel, 486 but the data for the bands are not convincing.…”

Section: Surface Electrophoresismentioning

confidence: 99%

Beyond Gel Electrophoresis: Microfluidic Separations, Fluorescence Burst Analysis, and DNA Stretching

et al. 2012

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Section: Surface Electrophoresismentioning

confidence: 99%

Beyond Gel Electrophoresis: Microfluidic Separations, Fluorescence Burst Analysis, and DNA Stretching

et al. 2012

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“…There are various non-invasive techniques available to detect such effects, namely colorimetric, 72 optical/fluorescence, 73 electrical, 74 or mechanical detection. 75 Some other phenomena which are frequently deployed in biosensing are dielectrophoresis (DEP), 76 electrophoresis, 77 polymerase chain reaction (PCR), 73 and live cell imaging, 78 which directly or indirectly assist cell - based biosensors.…”

Section: Classification Of Biosensorsmentioning

confidence: 99%

“…This is an internationally acclaimed technique to confirm the amplification of DNA samples through PCR, 87 while in capillary electrophoresis 88 the particles are made to move through single capillaries. Long strands of DNA molecules are also separated through yet another variant of electrophoresis which is surface electrophoresis 77 (the name given for electrophoresis on a flat surface).…”

Section: Classification Of Biosensorsmentioning

confidence: 99%

Biosensors on chip: A critical review from an aspect of micro/nanoscales

Bhatt

Bhattacharya

2019

Journal of Micromanufacturing

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Biosensors are a very well cherished research topic and have found an inseparable status from clinical diagnostics in specific and society at large. As the name suggests, biosensors or biological sensors are devices which detect the presence of biological entities or their constituents and derivatives. The field started decades ago and has matured quite well since its inception. The most important performance factors that are associated with biosensors are sensitivity, specificity, and limit of detection. The remaining efforts of the biosensor research domain focus on miniaturization aspects of the sensors. The growing advancements in this field have evolved the technology of biosensors to cater to full-scale diagnosis on microchips, bedside diagnostics, reduced cost, and increased speed of diagnostics. Biosensors are characterized through many different aspects; for example, one way is to classify them on the basis of the type of bio-recognition step that they would utilize or another way can be based on the type of detection scheme that they may integrate, etc. Depending on the bio-recognition layer’s properties, biosensors can be cell based, nucleic acid probe based, antibody/antigen based, or aptamer based, while depending on the type of detection scheme, biosensors can be viewed as colorimetric sensors, optical sensors, electrochemical sensors, mechanical sensors, etc. There are some other parallel areas of research like microfluidics and microelectromechanical systems where one of the main applications lies in the biosensor domain. This review article discusses the various aspects of biosensors, from their design, realization, to testing, along with various detection strategies. The assembly includes fabrication strategies particularly for microchip technology-based biosensing solutions, microchannels, integration to microfluidics, etc., while categorization deals with various kinds and applications of different biosensors.

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“…14 Further, efficient electrophoresis of DNA molecules across an orthogonal surface of a microchannel has been observed. 7 The structure of a DNA molecule on such narrow geometries, which are essentially a junction of two flat surfaces, appears to be 1D. The aim of the present work is to understand the structure and dynamics of a polymer at a junction where two flat surfaces intersect and form a groove.…”

Section: Introductionmentioning

confidence: 99%

Localization and stretching of polymer chains at the junction of two surfaces

Patra

Singh

2014

The Journal of Chemical Physics

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We present a molecular dynamics study on the stretching of a linear polymer chain that is adsorbed at the junction of two intersecting flat surfaces of varying alignments. We observe a transition from a two-dimensional to one-dimensional (1D) structure of the adsorbed polymer when the alignment, i.e., the angle between the two surfaces that form a groove, θ, is below 135°. We show that the radius of gyration of the polymer chain Rg scales as Rg ∼ N(3/4) with the degree of polymerization N for θ = 180° (planer substrate), and the scaling changes to Rg ∼ N(1.0) for θ < 135° in good solvents. At the crossover point, θ = 135°, the exponent becomes 1.15. The 1D stretching of the polymer chain is found to be 84% of its contour length for θ ⩽ 90°. The center of mass diffusion coefficient D decreases sharply with θ. However, the diffusion coefficient scales with N as D ∼ N(-1), and is independent of θ. The relaxation time τ, for the diffusive motion, scales as τ ∼ N(2.5) for θ = 180° (planar substrate), which changes to τ ∼ N(3.0) for θ ⩽ 90°. At the crossover point, the exponent is 3.4, which is slightly higher than the 1D value of 3.0. Further, a signature of reptation-like dynamics of the polymer chain is observed at the junction for θ ⩽ 90° due to its strong 1D localization and stretching.

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Surface electrophoresis of ds-DNA across orthogonal pair of surfaces

Abstract: Simulation study of the effects of surface chemistry and temperature on the conformations of ssDNA oligomers near hydrophilic and hydrophobic surfaces

Cited by 14 publications

References 19 publications

Beyond Gel Electrophoresis: Microfluidic Separations, Fluorescence Burst Analysis, and DNA Stretching

Beyond Gel Electrophoresis: Microfluidic Separations, Fluorescence Burst Analysis, and DNA Stretching

Biosensors on chip: A critical review from an aspect of micro/nanoscales

Localization and stretching of polymer chains at the junction of two surfaces

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