Surface engineered polyanhydride-based oral &lt;em&gt;Salmonella &lt;/em&gt;subunit nanovaccine for poultry

Sankar, Raman; Markazi, Ashley; Dhakal, Santosh; Lakshmanappa, Yashavanth Shaan; Gourapura, Suren R; Shanmugasundaram, R.; Senapati, Sujata; Narasimhan, Balaji; Selvaraj, Ramesh K.; Renukaradhya, Gourapura J.

doi:10.2147/ijn.s185588

Cited by 32 publications

(46 citation statements)

References 68 publications

(125 reference statements)

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“…At d16 (2d-post-challenge) birds vaccinated with commerical vaccine or CNP vaccine had lower (P<0.05) Salmonella load by 82.7% and 65.9%, respectively, when compared to that in the control group (Fig 4). Similar results were observed in a recent 2018 study, as a PNP vaccination cleared Salmonella in the ceca of 33% of birds in the study [29]. This identified that the synthesized vaccine decreased Samonella loads in the ceca of broilers.…”

Section: Plos Onesupporting

confidence: 90%

“…These results demonstrated that the CNP vaccine at doses above 500μg can induce an anti-Salmonella antigen-specific immune response in broilers. Similar results were observed in a recent study, where layer chickens that were orally immunized with a Salmonella polyanhydride nanoparticle (PNP) vaccine and challenged with S. Enteritidis had substantially higher OMPs-specific IgG response in the serum [29].…”

Section: Effect Of Cnp Vaccine Administration On Anti-omps And-flagelsupporting

confidence: 88%

“…At d25 (11d-post-challenge), serum, cloacal swabs, and bile samples were collected one bird/ pen (n = 3) and analyzed for anti-Salmonella OMPs and -flagellin IgG and IgA antibody response using an enzyme-linked immunosorbent assay (ELISA) as described previously [29]. High-binding-flat bottom 96-well plates (Greiner Bio-one, NC) were coated with either OMPs or flagellin (2 μg/mL for IgG and 7.5 μg/mL for IgA) diluted in 0.05 M sodium -bicarbonate coating buffer (9.6 pH), and incubated overnight at 4˚C.…”

Section: Antibody Response In Cnp-vaccine Administered Birdsmentioning

confidence: 99%

“…Primers were adapted from previous literature. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), Dube et al 2014 [31]; Ribosomal protein S13 (RPS13), Shanmugasundaram et al 2018; IL-1β, IFNγ, and IL-10, Shanmugasundaram et al 2019 [32]; IL-4, Renu et al 2018 [29].…”

Section: Cytokine Gene Expression In the Cecal Tonsils Liver And Spmentioning

confidence: 99%

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Efficacy of chitosan-based nanoparticle vaccine administered to broiler birds challenged with Salmonella

et al. 2020

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Two experiments were conducted to evaluate the immune response of broilers vaccinated with Salmonella chitosan-nanoparticle (CNP) vaccine and challenged with Salmonella. The Salmonella CNP vaccine was synthesized with Salmonella enterica outer membrane proteins (OMPs) and flagellin proteins. In Experiment I, birds were orally gavaged with PBS or 500, 1000, or 2000μg of CNP vaccine 1 and 7d-of-age. At 14d-ofage, birds were orally challenged with 1 X 10 5 CFU/bird of live S. Enteritidis (SE). Macrophage-nitrite production 11d-post-challenge was higher (P<0.05) in the 500μg group when compared to the control. At d14 (8h-post-challenge), broilers vaccinated with 1000μg CNP had higher (P<0.05) serum anti-OMPs IgG and IgA and cloacal anti-OMP IgA amounts. At 11d-post-challenge, birds vaccinated with 1000μg CNP vaccine had greater (P<0.05) bile anti-OMP and anti-flagellin IgA amounts. At 11d-post-challenge, birds administered 1000μg CNP vaccine has increased (P<0.05) IL-1β and IL-10 mRNA in cecal tonsils. In Experiment II, birds were orally gavaged with PBS or 1000μg CNP or a live commercial vaccine at 1 and 7d-of-age. At 14d-of-age, birds were orally challenged with 1 X 10 5 CFU/bird of live SE or S. Heidelberg (SH). Birds vaccinated with CNP showed higher (P<0.05) serum anti-OMPs IgG amounts at 8h-post-challenge. At 4d-post-SH challenge, birds vaccinated with CNP had higher (P<0.05) bile anti-flagellin IgA amounts. CNP decreased (P<0.05) anti-OMPs IgG levels in serum at 2d-post-SE challenge and 4d-post-SH or SE challenge. Salmonella Enteritidis loads in cecal content at 2d-post-challenge was decreased (P<0.05) by 65.9% in birds vaccinated with CNP, when compared to the control. Chitosan-nanovaccine had no adverse effects on bird's production performance. In conclusion, 1000μg CNP vaccine can induce a specific immune response against Salmonella and has the potential to mitigate SE cecal colonization in broiler birds.

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Section: Plos Onesupporting

confidence: 90%

Section: Effect Of Cnp Vaccine Administration On Anti-omps And-flagelsupporting

confidence: 88%

Section: Antibody Response In Cnp-vaccine Administered Birdsmentioning

confidence: 99%

Section: Cytokine Gene Expression In the Cecal Tonsils Liver And Spmentioning

confidence: 99%

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Efficacy of chitosan-based nanoparticle vaccine administered to broiler birds challenged with Salmonella

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“…The extracted antigens (OMPs and F-protein) analyzed by SDS-PAGE showed expected proteins corresponding to molecular weight were reported earlier. 45 Studies have established that the size and shape of NPs determine their biological properties. 46 The formulated CS NPs and OMPs-F-CS NPs were roughly 500- .…”

Section: Discussionmentioning

confidence: 99%

<p>Oral Deliverable Mucoadhesive Chitosan-<em>Salmonella</em> Subunit Nanovaccine for Layer Chickens</p>

Sankar¹,

Markazi²,

Dhakal³

et al. 2020

IJN

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Purpose: Salmonellosis in poultry is a serious economic burden. A major concern is the public health hazard caused by consumption of Salmonella-contaminated poultry products. Currently used Salmonella vaccines are ineffective in combating poultry Salmonellosis warranting the need of a potent vaccine, especially an oral vaccine that can elicit robust local intestinal immunity. Materials and Methods: A Salmonella subunit chitosan nanoparticles (NPs)-based vaccine was prepared that contained immunogenic outer membrane proteins (OMPs) and-flagellin (F) protein (OMPs-F-CS NPs). OMPs-F-CS NPs were administered as an oral vaccine in layer chickens and the resultant humoral and cell-mediated immune responses and localization of NPs were examined using standard detection methods. Results: We demonstrated targeting of surface F-protein coated chitosan NPs to immune cells when delivered orally to layer chickens, the particles were localized in ileal Peyer's patches. The OMPs-F-CS NPs vaccinated layer chickens had significantly higher OMPsspecific mucosal IgA production and lymphocyte proliferation response. The candidate vaccine increased the expression of toll-like receptor (TLR)-2, TLR-4, IFN-γ, TGF-ß and IL-4 mRNA expression in chicken cecal tonsils. Conclusion: Our study demonstrated that the chitosan-based oral Salmonella nanovaccine targets immune cells of chickens and induced antigen-specific B and T cell responses. This candidate oral Salmonella nanovaccine has the potential to mitigate Salmonellosis in poultry.

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Advances of Nanotechnology Toward Vaccine Development Against Animal Infectious Diseases

Wang

Gao

Wang

et al. 2023

Adv Funct Materials

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Vaccines are the most effective means of controlling infectious diseases. Increasingly epidemics have driven the development of vaccines for human use. However, insufficient attention has been paid to preventing animal epidemics. The spread of animal epidemics directly affects food and nutritional safety, the economy, and outbreaks of zoonotic diseases, which can eventually threaten human health. Reducing safety risks, saving production costs, adapting to mass vaccination, and improving immunogenicity are important that must be considered in animal vaccines. Nanotechnology in vaccine development provides unique advantages in meeting these challenges, both at in vitro preparation and in vivo immune response levels. In terms of in vitro vaccine formulation, nanoparticles offer the possibility to provide multiple antigen display sites, maintain antigen conformation, and improve vaccine stability, which facilitates the development of vaccines with enhanced immunogenicity and thermal stability. In terms of in vivo vaccine‐induced immunity, nanoparticles show superior immunostimulatory activity which facilitates multiple immune response processes, including antigen delivery, cellular uptake, antigen presentation, and lymphocyte activation, thus markedly augmenting vaccine‐mediated immune responses. Herein, the application of nanotechnology to vaccine development and the characteristics of animal vaccines are reviewed, aiming to provide general guidelines for the design of future vaccines against animal infectious diseases and promote harmonious coexistence of humans and animals.

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Surface engineered polyanhydride-based oral <em>Salmonella </em>subunit nanovaccine for poultry

Cited by 32 publications

References 68 publications

Efficacy of chitosan-based nanoparticle vaccine administered to broiler birds challenged with Salmonella

Efficacy of chitosan-based nanoparticle vaccine administered to broiler birds challenged with Salmonella

<p>Oral Deliverable Mucoadhesive Chitosan-<em>Salmonella</em> Subunit Nanovaccine for Layer Chickens</p>

Advances of Nanotechnology Toward Vaccine Development Against Animal Infectious Diseases

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