Surface Enhanced Infrared Absorption Spectroscopy

Osawa, Masatoshi; Ataka, Kenichi; Ikeda, Masahiko; Uchihara, Hiroshi; Nanba, Ryujiro

doi:10.2116/analsci.7.supple_503

Cited by 44 publications

(44 citation statements)

References 10 publications

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“…The infrared beam, provided from the interferometer of the FT-IR spectrometer (Bruker IFS 66 v/s, Bruker Optics, Ettlingen, Germany) was coupled into the single reflection silicon prism at an incident angle of 60 • . The beam was totally internally reflected and the intensity was measured by a mercury cadmium telluride (MCT) detector (Osawa et al, 1993;Ataka et al, 1996;Osawa, 1998, 1999;Osawa, 2002).…”

Section: Surface Modification-monitored By Surface-enhanced Infrared mentioning

confidence: 99%

Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen

Krassen

Stripp

Abendroth

et al. 2009

Journal of Biotechnology

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Section: Surface Modification-monitored By Surface-enhanced Infrared mentioning

confidence: 99%

Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen

Krassen

Stripp

Abendroth

et al. 2009

Journal of Biotechnology

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“…The 855 cm' band is probably assigned to the C-C stretching vibration. 16 The peak intensity of the 1590 cm-1 band in spectrum a is 8-times larger than that of spectrum b, despite the 10-times smaller amount of the molecule deposited. From these results, the enhancement factor of 80 is calculated.…”

Section: Standard Spectra Of the Components Of Secretionsmentioning

confidence: 92%

Trace Analysis of Human Skin Secretions by Surface-Enhanced Infrared Spectroscopy: Detection of Lactate

Nishikawa¹,

Fujiwara²,

Osawa

et al. 1993

ANAL. SCI.

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Surface-enhanced infrared spectroscopy was applied to the trace analysis of secretions on human skin. The secretions were transferred onto silver-coated barium fluoride substrates by pressing the substrates on the skin at several parts of the human body (wrist, palm, cheek, abdomen and sole). The infrared spectra of the substrates were measured by the transmission mode.Small amounts of lactate, triglycerides, fatty acids and fatty acid esters were identified from the secretions.For detecting lactate, the sole is the best sampling point on the human body. Lactate is a product of anaerobic metabolism in the glycolytic pathway.1 Since lactate plays an important role in energy metabolism, the determination of lactate in biological samples has been the subject of intensive study.2 Recently, Karube et al. showed a remarkable increase of lactate in human secretions after under going hard exercises.3 They also suggested that there exists an intimate correlation between fatigue and lactate production. The degree of fatigue may thus be estimated from the amounts of lactate found in sweat.So far, lactate has been analyzed using electrochemical biosensors.4,5 In conventional sensors, the concentration changes of hydrogen peroxide or oxygen caused by the enzymatic oxidation of lactate is usually detected. However, this method is relatively complicated and suffers from several types of interference due to reducing substances, such as ascorbic acid. In the present work, we applied a simple and sensitive infrared spectroscopy technique to the trace analysis of lactate on human skin.Infrared spectroscopy is useful for the characterization of organic materials. Though the development of Fourier-transform infrared (FT-IR) spectrometers has led to a marked improvement in the sensitivity of this method, the sensitivity is still insufficient for the trace analysis of lactate on human skin. In this study, surface-enhanced infrared absorption (SEIRA) spectroscopy6-9 was used to provide a further improvement in the sensitivity of FT-IR. SEIRA is a phenomenon in which the infrared absorption intensity of molecules adsorbed on or near to very thin metal films of silver, gold, copper, or indium is remarkably ExperimentalBarium fluoride (BaF2) plates (20X20X4 mm) were used as testing substrates for the SEIRA measurements. After the substrates were cleaned in ethanol and acetone, silver films were deposited from a tungsten basket in a JEOL JEE 4C vacuum evaporator at a pressure lower than 5X10-5 Torr. Although the largest absorption enhancement was observed on silver films of 8 -10 nm in thickness (estimated with a Tencor profilometer), the band shape was slightly distorted. To avoid this, the silver thickness was restricted to 6 nm. The metal films were not continuous, but consisted of metal particles of about 30 nm in average diameter.The silver-coated substrates were pressed against human skin at the wrist, palm, cheek, abdomen, or sole at a pressure of 25 g/ cm2 for 20 s; the secretions on the skins were transferred to the substrate. Th...

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“…24 In general, the IR band enhancement for chemisorbed molecules is higher than that for physisorbed molecules as the latter are more randomly orientated. In the adsorption model developed by Osawa and coworkers in terms of the chemisorption of carboxylate moiety on a metal particle that was originally presented by Hatta et al 15,25,26 the islands comprising the metal film are assumed to be ellipsoids of rotation, and the organic molecule adsorbs perpendicular to the surface through the oxygen atoms. 27 On the basis of the model, the adsorption of carboxylic moiety (COO) on a metal surface is achieved through two oxygen atoms footed on the surface (bidentate adsorption), forming a triangle-like adsorption.…”

Section: -H S T R E T C H I N G -mentioning

confidence: 99%

Infrared absorption enhancement of fluorinated poly‐acrylate thin films on Co‐alloy substrate

Zhang

2006

Surface & Interface Analysis

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Surface Enhanced Infrared Absorption Spectroscopy

Cited by 44 publications

References 10 publications

Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen

Immobilization of the [FeFe]-hydrogenase CrHydA1 on a gold electrode: Design of a catalytic surface for the production of molecular hydrogen

Trace Analysis of Human Skin Secretions by Surface-Enhanced Infrared Spectroscopy: Detection of Lactate

Infrared absorption enhancement of fluorinated poly‐acrylate thin films on Co‐alloy substrate

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