Surface-enhanced laser desorption–ionization retentate chromatography™ mass spectrometry (SELDI–RC–MS): a new method for rapid development of process chromatography conditions

Weinberger, Scot R.; Boschetti, Egisto; Santambien, Patrick; Brenac, Virginie

doi:10.1016/s1570-0232(02)00564-0

Cited by 64 publications

(43 citation statements)

References 23 publications

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“…12 Protein chip technology coupled with surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) has offered a sensitive, high throughput and rapid way for screening protein expression within complex biological specimens. 13,14 In a previous study, we identified five potential biomarkers in the serum of women with endometriosis using SELDI-TOF-MS protein chip array technology, and this diagnostic system was shown to distinguish endometriosis from validation samples with a sensitivity of 91.7% and a specificity of 90.0%. 15 The present study was designed to search for different mitochondrial proteins in samples of eutopic endometrium from patients with adenomyosis or endometriosis, and to compare the proteins between the two diseases.…”

Section: Mitochondrial Proteins In Adenomyosis and Endometriosismentioning

confidence: 99%

Differences in Mitochondrial Proteins in the Eutopic Endometrium of Patients with Adenomyosis and Endometriosis Identified Using Surface-enhanced Laser Desorption/Ionization Time-of-flight Mass Spectrometry

et al. 2010

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Adenomyosis and endometriosis have a similar pathogenesis; indeed, adenomyosis has been considered by some as a variant of endometriosis ('internal endometriosis'). This study aimed to detect differences in mitochondrial proteins in eutopic endometrial samples from women with adenomyosis (n = 13) and endometriosis (n = 24), and from control patients (n = 29) using surface-enhanced laser desorption/ ionization time-of-flight mass spectrometry (SELDI-TOF-MS) protein chip technology. A total of 82 and 78 mitochondrial protein peaks were found in adenomyosis and endometriosis individuals, respectively. Of these, 14 were common to women with adenomyosis and women with endometriosis, although only one of these (mass-to-charge [m/z] ratio 3499) was significantly different between the adenomyosis and endometriosis groups. It is concluded that, compared with control patients, there are differences in the mitochondrial proteins isolated from the eutopic endometrium of patients with adenomyosis and those with endometriosis. Although the changes in mitochondrial proteins in eutopic endometrium from patients with adenomyosis and endometriosis were largely similar, significant differences were also detected. Further identification of these proteins and elucidation of the differences will help towards the differential diagnosis of adenomyosis and endometriosis and new therapeutic approaches.

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Section: Mitochondrial Proteins In Adenomyosis and Endometriosismentioning

confidence: 99%

Differences in Mitochondrial Proteins in the Eutopic Endometrium of Patients with Adenomyosis and Endometriosis Identified Using Surface-enhanced Laser Desorption/Ionization Time-of-flight Mass Spectrometry

et al. 2010

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“…Isolation and identification can be performed by either two-dimensional electrophoresis (14) or ProteinChip technology (15), where the isolated protein is digested by proteolytic enzyme cleavage, and the mass values of the fragments generated are used for peptide mapping to identify the protein of interest by a database search. Furthermore, for confirmatory identification, selected peptides can be sequenced by collision-induced dissociation using a ProteinChip Interface coupled to a tandem mass spectrometer (16,17).…”

Section: Introductionmentioning

confidence: 99%

A Technical Triade for Proteomic Identification and Characterization of Cancer Biomarkers

et al. 2004

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Biomarkers are needed to elucidate the biological background and to improve the detection of cancer. Therefore, we have analyzed lasermicrodissected cryostat sections from head and neck tumors and adjacent mucosa on ProteinChip arrays. Two differentially expressed proteins (P ‫؍‬ 3.34 ؋ 10 ؊5 and 4.6 ؋ 10 ؊5) were isolated by two-dimensional gel electrophoresis and identified as S100A8 (calgranulin A) and S100A9 (calgranulin B) by in-gel proteolytic digestion, peptide mapping, tandem mass spectrometry analysis, and immunodepletion assay. The relevance of these single marker proteins was evaluated by immunohistochemistry. Positive tissue areas were reanalyzed on ProteinChip arrays to confirm the identity of these proteins. As a control, a peak with low P was identified as calgizzarin (S100A11) and characterized in the same way. This technical triade of tissue microdissection, ProteinChip technology, and immunohistochemistry opens up the possibility to find, identify, and characterize tumor relevant biomarkers, which will allow the movement toward the clonal heterogeneity of malignant tumors. Taking this approach, proteins were identified that might be responsible for invasion and metastasis.

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“…For the isolation, different techniques are in use. As well as the two-dimensional gel electrophoresis (2-DE) (24) applied in this study, the ProteinChip system can also be used for the on-spot development of purification protocols and the monitoring of the subsequent purification process (25). In either case, the isolated protein is digested by enzymatic cleavage.…”

mentioning

confidence: 99%

Biomarker Discovery and Identification in Laser Microdissected Head and Neck Squamous Cell Carcinoma with ProteinChip® Technology, Two-dimensional Gel Electrophoresis, Tandem Mass Spectrometry, and Immunohistochemistry

Melle

Ernst

Schimmel

et al. 2003

Molecular & Cellular Proteomics

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Head and neck cancer is a frequent malignancy with a complex, and up to now not clear etiology. Therefore, despite of improvements in diagnosis and therapy, the survival rate with head and neck squamous-cell carcinomas is poor. For a better understanding of the molecular mechanisms behind the process of tumorigenesis and tumor progression, we have analyzed changes of protein expression between microdissected normal pharyngeal epithelium and tumor tissue by ProteinChip® technology. For this, cryostat sections from head and neck tumors (n ‫؍‬ 57) and adjacent mucosa (n ‫؍‬ 44) were laser-microdissected and analyzed on ProteinChip arrays. The derived mass spectrometry profiles exhibited numerous statistical differences. One peak significantly higher expressed in the tumor (p ‫؍‬ 0.000029) was isolated by twodimensional gel electrophoresis and identified as annexin V by in-gel proteolytic digestion, peptide mapping, tandem mass spectrometry analysis, and immuno-deplete assay. The relevance of this single marker protein was further evaluated by immunohistochemistry. Annexinpositive tissue areas were re-analyzed on ProteinChip arrays to confirm the identity of this protein. In this study, we could show that biomarker in head and neck cancer can be found, identified, and assessed by combination of ProteinChip technology, two-dimensional gel electrophoresis, and immunohistochemistry. In our experience, however, such studies only make sense if a relatively pure microdissected tumor tissue is used. Only then minute changes in protein expression between normal

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Surface-enhanced laser desorption–ionization retentate chromatography™ mass spectrometry (SELDI–RC–MS): a new method for rapid development of process chromatography conditions

Cited by 64 publications

References 23 publications

Differences in Mitochondrial Proteins in the Eutopic Endometrium of Patients with Adenomyosis and Endometriosis Identified Using Surface-enhanced Laser Desorption/Ionization Time-of-flight Mass Spectrometry

Differences in Mitochondrial Proteins in the Eutopic Endometrium of Patients with Adenomyosis and Endometriosis Identified Using Surface-enhanced Laser Desorption/Ionization Time-of-flight Mass Spectrometry

A Technical Triade for Proteomic Identification and Characterization of Cancer Biomarkers

Biomarker Discovery and Identification in Laser Microdissected Head and Neck Squamous Cell Carcinoma with ProteinChip® Technology, Two-dimensional Gel Electrophoresis, Tandem Mass Spectrometry, and Immunohistochemistry

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