Surface‐enhanced Raman scattering assessment of DNA from leaf tissues adsorbed on silver colloidal nanoparticles

Abstract

In this work, the surface‐enhanced Raman scattering (SERS) spectra of seven genomic DNAs from leaves of chrysanthemum (Dendranthema grandiflora Ramat.), common sundew (Drosera rotundifolia L.), edelweiss (Leontopodium alpinum Cass), Epilobium hirsutum L., Hypericum richeri ssp. transsilvanicum (Čelak) Ciocârlan, rose (Rosa x hybrida L.) and redwood (Sequoia sempervirens D. Don. Endl.), respectively, have been analyzed in the wavenumber range 200–1800 cm−1. The surface‐enhanced Raman vibrational modes for each of these cases, spectroscopic band assignments and structural interpretations of genomic DNAs are reported. A high molecular structural information content can be found in the SERS spectra of these DNAs from leaf tissues. Based on this work, specific plant DNA–ligand interactions or accurate local structure of DNA might be further investigated using surface‐enhanced Raman spectroscopy. Besides, this study will generate information which is valuable in the development of label‐free DNA detection for chemical probing in living cell. Copyright © 2013 John Wiley & Sons, Ltd.

Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation

“…formation of DNA-silver bonds). On the other hand, a selective enhancement of vibrational bands is a consequence of surface selection rules, which concerns chemically as well as physically attached molecules ( [4] and references therein).…”

“…The pH value of all measured SERS mixtures, containing colloidal nanoparticles and DNA extracts was of 6.5. We did not observe any change of color in the DNA-nanoparticle systems prepared for SERS study, this being a proof that the silver remains colloidal and is not aggregated upon interaction with genomic nucleic acids [4,6,7]. Each sample was prepared by adding 10 ll of stock DNA solutions, respectively, to 500 ll silver colloid into a 1-ml glass cuvette.…”

“…The pH value of the silver colloid, measured immediately after preparation, was found to be 7.5. However, after one day the pH value lowered to 6.5, remaining constant at this value for the next weeks [4,7]. The pH value of all measured SERS mixtures, containing colloidal nanoparticles and DNA extracts was of 6.5.…”

“…In a separate recipient, 0.017 g of hydroxylamine hydrochloride were solved in 10 ml water, followed by the addition of 1.1 ml sodium hydroxide solution (1%). The hydroxylamine/sodium hydroxide solution was then added rapidly to the silver nitrate solution under vigorous stirring [4,7]. After few seconds a gray-brown colloidal solution resulted and it was further stirred for 10 min.…”

“…Ag, Cu, Au) nanostructures with sizes in the order of tens of nanometers [3]. Besides, the charge transfer (CT) mechanism is associated with a charge transfer process between the adsorbed analyte and the metal surface ( [4] and references therein). Therefore, vibrations involved in the charge transfer process are enhanced ( [5] and references therein).…”