Surface-enhanced Raman spectral characterization of antifungal activity of selenium and zinc based organometallic compounds

Raza, Ali; Parveen, Soneya; Majeed, Muhammad Irfan; Nawaz, Haq; Javed, Muhammad Rizwan; Iqbal, Muhammad Adnan; Rashid, Nosheen; Haider, Muhammad Zeeshan; Ali, Muhammad; Sabir, Amina; Hasan, Hafiz Mahmood ul; Majeed, Beenish

doi:10.1016/j.saa.2022.121903

Cited by 9 publications

(5 citation statements)

References 47 publications

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“…The decrease in intensity at 1157 cm –1 (C–N stretching in aromatic amino acids) suggests structural changes in proteins, possibly due to oxidative stress or the degradation of protein backbones. The weakened SERS response to cell wall carbohydrates implies a stronger interaction between the cell wall and proteins, emphasizing the significance of phenylalanine in the fungal outer layers and inner walls . The observed alterations in the SERS bands suggest significant biological implications.…”

Section: Resultsmentioning

confidence: 96%

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Surface-Enhanced Raman Spectroscopy for Monitoring the Biochemical Changes Due to DNA Mutations Induced by CRISPR-Cas9 Genome Editing in the Aspergillus niger Fungus

Wasim,

Ghaffar,

Javed

et al. 2024

ACS Omega

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In this study, surface-enhanced Raman spectroscopy (SERS) technique, along with principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA), is used as a simple, quick, and cost-effective analysis method for identifying biochemical changes occurring due to induced mutations in the Aspergillus niger fungus strain. The goal of this study is to identify the biochemical changes in the mutated fungal cells (cell mass) as compared to the control/nonmutated cells. Furthermore, multivariate data analysis tools, including PCA and PLS-DA, are used to further confirm the differentiating SERS spectral features among fungal samples. The mutations are caused in A. niger by the clustered regularly interspaced palindromic repeat CRISPR-Cas9 genomic editing method to improve their biotechnological potential for the production of cellulase enzyme. SERS was employed to detect the changes in the cells of mutated A. niger fungal strains, including one mutant producing low levels of an enzyme and another mutant producing high levels of the enzyme as a result of mutation as compared with an unmutated fungal strain as a control sample.

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Section: Resultsmentioning

confidence: 96%

“…The weakened SERS response to cell wall carbohydrates implies a stronger interaction between the cell wall and proteins, emphasizing the significance of phenylalanine in the fungal outer layers and inner walls. 27 The observed alterations in the SERS bands suggest significant biological implications.…”

Section: Resultsmentioning

confidence: 99%

Surface-Enhanced Raman Spectroscopy for Monitoring the Biochemical Changes Due to DNA Mutations Induced by CRISPR-Cas9 Genome Editing in the Aspergillus niger Fungus

Wasim,

Ghaffar,

Javed

et al. 2024

ACS Omega

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“…The mixture was heated on a hot plate using a magnetic stirrer for approximately an hour until gray-colored silver nanoparticles in the size range of 65 × 45 nm were formed, as characterized and reported earlier. 51 The SEM analysis was performed to characterize the Ag NPs as shown in Fig. S1 † and discussed in Section 2.2 in ESI.…”

Section: Methodsmentioning

confidence: 99%

Surface-enhanced Raman spectroscopy for studying the interaction of organometallic compound bis(1,3-dihexylimidazole-2-yl) silver(i) hexafluorophosphate (v) with the biofilm of Escherichia coli

Tabussam,

Shehnaz,

Majeed

et al. 2024

RSC Adv.

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“…This resulted in gray-colored Ag-NPs. The NPs were further characterized using TEM, SEM, and UV–vis spectrometry. ,, …”

Section: Methodsmentioning

confidence: 99%

“…The NPs were further characterized using TEM, SEM, and UV−vis spectrometry. 18,23,34 4.4. Acquisition of the SERS Spectra.…”

Section: Synthesis Of Nanoparticlesmentioning

confidence: 99%

Synthesis, Characterization, and Evaluation of Antifungal Activity of 1-Butyl-3-hexyl-1H-imidazol-2(3H)-selenone by Surface-Enhanced Raman Spectroscopy

Huda,

Wasim,

Nawaz

et al. 2023

ACS Omega

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In the present research work, a selenium Nheterocyclic carbene (Se−NHC) complex/adduct was synthesized and characterized by using different analytical methods including FT-IR, 1 HNMR, and 13 CNMR. The antifungal activity of the Se−NHC complex against Aspergillus flavus (A. flavus) fungus was investigated with disc diffusion assay. Moreover, the biochemical changes occurring in this fungus due to exposure of different concentrations of the in-house synthesized compound are characterized by surfaceenhanced Raman spectroscopy (SERS) and are illustrated in the form of SERS spectral peaks. SERS analysis yields valuable information about the probable mechanisms responsible for the antifungal effects of the Se−NHC complex. As demonstrated by the SERS spectra, this Se−NHC complex caused denaturation and conformational changes in the proteins as well as decomposition of the fungal cell membrane. The SERS spectra were analyzed using two chemometric tools such as principal component analysis (PCA) and partial least-squares discriminant analysis (PLS-DA). The fungal samples' SERS spectra were differentiated using PCA, while various groups of spectra were discriminated with ultrahigh sensitivity (98%), high specificity (99.7%), accuracy (100%), and area under the receiver operating characteristic curve (87%) using PLS-DA.

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Surface-enhanced Raman spectral characterization of antifungal activity of selenium and zinc based organometallic compounds

Cited by 9 publications

References 47 publications

Surface-Enhanced Raman Spectroscopy for Monitoring the Biochemical Changes Due to DNA Mutations Induced by CRISPR-Cas9 Genome Editing in the Aspergillus niger Fungus

Surface-Enhanced Raman Spectroscopy for Monitoring the Biochemical Changes Due to DNA Mutations Induced by CRISPR-Cas9 Genome Editing in the Aspergillus niger Fungus

Surface-enhanced Raman spectroscopy for studying the interaction of organometallic compound bis(1,3-dihexylimidazole-2-yl) silver(i) hexafluorophosphate (v) with the biofilm of Escherichia coli

Synthesis, Characterization, and Evaluation of Antifungal Activity of 1-Butyl-3-hexyl-1H-imidazol-2(3H)-selenone by Surface-Enhanced Raman Spectroscopy

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