Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)

Fazio, Alejandra Teresa; Mamián-López, Mónica B.; Temperini, Márcia L. A.; Faria, Dalva Lúcia Araújo de

doi:10.1016/j.vibspec.2018.06.002

Cited by 11 publications

(5 citation statements)

References 25 publications

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“…The score scatter diagrams (Fig. [7][8][9][10][11] showed that the spectra of the studied lamentous fungi samples were distinguishable from the background spectra. However, the score scatter diagram of Trichoderma atroviride indicated a relatively large scattering of points, which hindered the resolution of spectra with a surface concentration of 2 10 5 cm -2 from the background spectra due to measurement inaccuracies.…”

Section: Discussionmentioning

confidence: 99%

“…A study by Salman et al (Salman et al 2010) shows differences between examined fungi genera using FTIR ATR spectroscopy. Surface Enhanced Raman Spectroscopy (SERS) was employed to discriminate fungi found in bio lms formed on earthen architecture walls in the region known as Paraíba Valley (or São Paulo Historical Valley) (Fazio et al 2018).…”

Section: Introductionmentioning

confidence: 99%

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Microbial Contaminated Paper Substrate – UV-Vis-NIR spectra of Model Systems

Gal,

Paračková,

Kaliňáková

et al. 2023

Preprint

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This study aims to study the possibility of distinguishing the UV-Vis-NIR spectra of filamentous fungi on a paper substrate from the background. Model samples of five filamentous fungi were used: Alternaria alternata, Aspergillus niger, Cladosporium herbarum, Penicillium chrysogenum, and Trichoderma atroviride. The model samples were cultivated on paper substrates, and two methods, Cross Validation (CV) and Principal Component Analysis (PCA), were utilised to compare their spectra with the reference background spectra. The results of the CV analysis indicated that certain combined spectra sets of Cladosporium herbarum, Penicillium chrysogenum, Trichoderma atroviride, Aspergillus niger, and Alternaria alternata, at specific surface concentrations, exhibited two active components, signifying distinguishable differences from the background spectra. Additionally, the score scatter diagrams derived from PCA revealed clusters of samples, further confirming the distinguishability of the filamentous fungi spectra from the background. However, for Trichoderma atroviride, the scatter diagram demonstrated a relatively large scattering of points, impeding the resolution of spectra with a surface concentration of 2 105 cm-2 due to measurement inaccuracies. Based on the combined results of CV and PCA, the study concluded that the lower threshold of measurability for UV-Vis-NIR spectra varied among the different filamentous fungi. For example, Cladosporium herbarum, Penicillium chrysogenum, and Trichoderma atroviride exhibited a threshold around a surface concentration of 2 106 cm-2, while Aspergillus niger had a threshold around 2 105 cm-2, and Alternaria alternata had a threshold around 2 103 cm-2. In summary, this research provides insights into the distinguishability of filamentous fungi spectra on the paper substrate from background spectra using spectral analysis techniques, offering potential applications in fungal identification and characterisation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbial Contaminated Paper Substrate – UV-Vis-NIR spectra of Model Systems

Gal,

Paračková,

Kaliňáková

et al. 2023

Preprint

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“…The results showed that bands between 1300 and 1600 cm −1 indicate the presence of CH 2 and CH 3 , and lipid deformation occurs on the surface of mycelium. In contrast, peaks from 1000 to 1050 cm −1 can be linked to the presence of lipids, fatty acids, and phenylalanine, as indicated by Fazio et al [78]. The 1600 cm −1 peaks indicate an unsaturated fatty acid group attributed to the C=C expansion vibration [79].…”

Section: Chemical Structure Of the Fungal Myceliummentioning

confidence: 99%

“…P. pedernalense EAN604 and C. geniculatus EAN403 have a high peak between 2850 and 2950 cm −1, indicating cyclohexane compounds, such as symmetric CH 2 stretching and asymmetric CH 3 stretching, as well as polyethylene, polypropylene, and methine in these fungal strains. Fazio et al [78]. The 1600 cm −1 peaks indicate an unsaturated fatty acid group attributed to the C=C expansion vibration [79].…”

Section: Chemical Structure Of the Fungal Myceliummentioning

confidence: 99%

New Fungal Strains from Peat Soil in Malaysia: Morphological and Molecular Characteristics

et al. 2023

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Fungi have unique properties and are used in many areas of agriculture and industry because they can produce different enzymes. This study aims to study the fungal diversity in peat soil from Pontian in Johor, Malaysia. The fungal isolates were described on different culture media and on a new culture medium called EVA medium and were identified using the phenotypical characteristics and molecular properties of the D1/D2 domain of the 28S large subunit ribosomal RNA (28S rRNA) and ITS (ITS1-ITS4) rDNA regions. The results revealed that 14 fungal species (15 isolates) were identified, among them, 6 were categorized as newly isolated strains and recorded in Malaysia; these include Aspergillus arenarioides EAN603, A. iizukae EAN605, Paraconiothyrium brasiliense EAN202, Parengyodontium album EAN602, Penicillium pedernalense EAN604, and Purpureocillium lilacinum EAN601. The cultural, morphological, microstructure, and molecular characteristics of these new strains have been described in this study. It was noted that the EVA medium exhibited a moderate support for fungal growth and sporulation compared to other culture media. Furthermore, the efficiency of the new medium as an enrichment medium to isolate fungi from peat soils with high ligninolytic content was discussed.

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“…For the identification of dyes and pigments in real applications such as forensic and heritage science, where it is important to save the exterior appearance of the objects, non-destructive, highly sensitive methods of sample preparation are preferred [ 30 ]. The existing methodology and procedures for detection of low concentrations of trace analytes by SERS and MEF suggest the use of preprepared nanostructures with a dose of extracted suspensions of the real mixtures of dyes and pigments deposited above the nanostructure [ 31 , 32 , 33 ]. This means, for example, that some segment of the picture must be separated and irrevocably destroyed to complete the identification and scientific investigations.…”

Section: Introductionmentioning

confidence: 99%

Aerosol Dry Printing for SERS and Photoluminescence-Active Gold Nanostructures Preparation for Detection of Traces in Dye Mixtures

et al. 2022

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We proposed a novel method of nanostructure preparation for observation of surface-enhanced Raman spectroscopy (SERS) and metal-enhanced fluorescence (MEF) based on the deposition of gold nanoparticles (GNPs) above the thin dye film by dry aerosol printing. We detected various enhanced SERS and MEF signals of films of malachite green (MG) and rhodamine B (RhB) mixtures, depending on the surface packing density of Au NPs on the strip, and found the optimum one to achieve the 3.5 × 105 SERS enhancement. It was shown that statistical methods of chemometrics such as projection on latent structures provided the opportunity to distinguish SERS of MG from 100 ppm RhB in a mixture, whereas separation of MEF signals are feasible even for a mixture of MG and 1 ppm RhB due to two-photon excitation.

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Surface enhanced Raman spectroscopy and cultural heritage biodeterioration: Fungi identification in earthen architecture from Paraíba Valley (São Paulo, Brazil)

Cited by 11 publications

References 25 publications

Microbial Contaminated Paper Substrate – UV-Vis-NIR spectra of Model Systems

Microbial Contaminated Paper Substrate – UV-Vis-NIR spectra of Model Systems

New Fungal Strains from Peat Soil in Malaysia: Morphological and Molecular Characteristics

Aerosol Dry Printing for SERS and Photoluminescence-Active Gold Nanostructures Preparation for Detection of Traces in Dye Mixtures

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