Surface enhanced Raman spectroscopy as a point-of-care diagnostic for infection in wound effluent

Ghebremedhin, Meron; Yesupriya, Shubha; Crane, Nicole J.

doi:10.1117/12.2212667

SPIE Proceedings

2016

DOI: 10.1117/12.2212667

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Surface enhanced Raman spectroscopy as a point-of-care diagnostic for infection in wound effluent

Meron Ghebremedhin¹,

Shubha Yesupriya²,

Nicole J. Crane³

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Cited by 2 publications

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Spectroscopic analysis of bacterial photoreactivation

Abady,

Karpourazar,

Krishnamoorthi

et al. 2024

Photochem & Photobiology

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With the rise of bacterial infections and antibiotic resistance, spectroscopic devices originally developed for bacterial detection have shown promise to rapidly identify bacterial strains and determine the ratio of live to dead bacteria. However, the detection of the photoreactivated pathogens remains a critical concern. This study utilizes fluorescence and Raman spectroscopy to analyze bacterial responses to UV irradiation and subsequent photoreactivation. Our experimental results reveal limitations in fluorescence spectroscopy for detecting photoreactivated bacteria, as the intense fluorescence of tryptophan and tyrosine amino acids masks the fluorescence emitted by thymine molecules. Conversely, Raman spectroscopy proves more effective, showing a detectable decrease in band intensities ofE. colibacteria at 1248 and 1665 cm−1after exposure to UVC radiation. Subsequent UVA irradiation results in the partial restoration of these band intensities, indicating DNA repair and bacterial photoreactivation. This enhanced understanding aims to improve the accuracy and effectiveness of these spectroscopic tools in clinical and environmental settings.

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Spectroscopic analysis of bacterial photoreactivation

Abady,

Karpourazar,

Krishnamoorthi

et al. 2024

Photochem & Photobiology

View full text Add to dashboard Cite

show abstract

Application of Vibrational Spectroscopy and Imaging to Point-of-Care Medicine: A Review

et al. 2018

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Surface enhanced Raman spectroscopy as a point-of-care diagnostic for infection in wound effluent

Cited by 2 publications

References 36 publications

Spectroscopic analysis of bacterial photoreactivation

Spectroscopic analysis of bacterial photoreactivation

Application of Vibrational Spectroscopy and Imaging to Point-of-Care Medicine: A Review

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