Surface enhanced Raman spectroscopy of RNA samples extracted from blood of hepatitis C patients for quantification of viral loads

Nasir, Saira; Majeed, Muhammad Irfan; Nawaz, Haq; Rashid, Nosheen; Ali, Saqib; Farooq, Sidra; Kashif, Mohammad; Rafiq, Sidra; Bano, Saira; Ashraf, Muhammad; Abubakar, Muhammad; Ahmad, Shamsheer; Rehman, Asma; Amin, Imran

doi:10.1016/j.pdpdt.2020.102152

Cited by 28 publications

(19 citation statements)

References 80 publications

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“…[70] with Copyright (2018) by American Chemical Society quantification of the viral load [86]. Further, the limit-ofdetection (LOD) for viral particles was determined via SERS using an in vitro blood spiking experiment [87][88][89][90][91]. The resulting LOD curve was applied to detect and differentiate Hepatitis B and C infected patients from the healthy donors [87][88][89][90][91].…”

Section: Detection Of Viral Loadmentioning

confidence: 99%

“…Further, the limit-ofdetection (LOD) for viral particles was determined via SERS using an in vitro blood spiking experiment [87][88][89][90][91]. The resulting LOD curve was applied to detect and differentiate Hepatitis B and C infected patients from the healthy donors [87][88][89][90][91]. Nasir et al used a SERS-based approach to quantify viral loads of human papilloma virus (HPV) in blood samples by investigating the extracted RNA [90] and Santos et al quantified the viral load of dengue virus serotype 3 (DENV-3) in plasma and blood samples using ATR FTIR spectroscopy [92].…”

Section: Detection Of Viral Loadmentioning

confidence: 99%

“…The resulting LOD curve was applied to detect and differentiate Hepatitis B and C infected patients from the healthy donors [87][88][89][90][91]. Nasir et al used a SERS-based approach to quantify viral loads of human papilloma virus (HPV) in blood samples by investigating the extracted RNA [90] and Santos et al quantified the viral load of dengue virus serotype 3 (DENV-3) in plasma and blood samples using ATR FTIR spectroscopy [92]. A SERS-sensor including virus-traps in form of nano-forests of an oblique gold-nanoneedles array (GNA's) coated with ACE-2 receptors allowed the detection of SARS-CoV-2 from urine samples with a viral load of 80 copies/ mL within 5 minutes (Figure 13) [93].…”

Section: Detection Of Viral Loadmentioning

confidence: 99%

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Understanding viruses and viral infections by biophotonic methods

Ramoji

Pahlow

Pistiki

et al. 2022

Transl Biophotonics

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In the last few decades outbreaks of viral infections have often challenged the world‐wide health infrastructure and caused a significant financial burden as well as human suffering despite progress in diagnostic technologies. The recent outbreaks of the Ebola virus in the African continent, the Zika virus in the American continent, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), influenza A and lately severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) viral infections have repeatedly highlighted the importance of technological advancement enabling a better understanding of virions. In this review, we systematically discuss different aspects of virions and how their properties and functions can be studied using different light‐based technologies. We focus on virion classification, detection and interactions with the host's immune system. Further, the potential of advanced biophotonic methods, for example, Raman, infrared reflection, absorption and fluorescence spectroscopy, advanced microscopic techniques and biosensor‐based approaches for diagnosing viral infections, investigating therapeutics and vaccine development are described. Although significant advancements have already been made in photonic technologies, which even enable visualizing virion‐host interactions on single‐cell level, the continuous evolution of viruses demands further progress in biophotonic solutions for fast, affordable and robust health monitoring devices for screening viral infections.

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Section: Detection Of Viral Loadmentioning

confidence: 99%

Section: Detection Of Viral Loadmentioning

confidence: 99%

Section: Detection Of Viral Loadmentioning

confidence: 99%

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Understanding viruses and viral infections by biophotonic methods

Ramoji

Pahlow

Pistiki

et al. 2022

Transl Biophotonics

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“…Immobilising a specific peptide nucleic acid on a gold nanoparticle surface as a highly selective bio-receptor, Raman spectroscopy and Square wave voltammetry was employed for the detection of HCV – RNA with a sensitivity of ~ 1 × 10 3 IU/mL. Furthermore, Batool et al used a non selective SERS approach to measure the PCR extracted DNA products from the blood serum of HBV positive patients [166] , and Nasir et al used a similar methodology for analysis of the blood of HCV positive patients [167] .…”

Section: Vibrational Spectroscopic Analysis Of Viruses and Viral Infe...mentioning

confidence: 99%

Contributions of vibrational spectroscopy to virology: A review

Chaudhary¹,

Jackson²,

Denning³

et al. 2022

Clinical Spectroscopy

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“…Although this approach is less specific than a nanosensor-based approach, it still provides quite promising results when combined with highly advanced data analysis. Some of the latest examples of label-free SERS applications are: measuring the urine of a patient to detect and monitor the risk of a relapse of prostate cancer (Ma et al, 2021); characterizing the hepatitis C viral (HCV) RNA extracted from different blood samples of patients with HCV infection (Nasir et al, 2020); diagnosing ulcerative colitis in blood plasma (Tefas et al, 2021); and assessing the effectiveness of antiplatelet therapy (Zyubin et al, 2020). In labelfree experiments, unfortunately, it is usually impossible to find a new band differentiating two sets of samples.…”

Section: Label-free Direct Detection Via Sersmentioning

confidence: 99%

Applications of Surface-Enhanced Raman Scattering in Biochemical and Medical Analysis

Szaniawska

Kudelski

2021

Front. Chem.

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In this mini-review, we briefly describe certain recently developed applications of the surface-enhanced Raman spectroscopy (SERS) for determining various biochemically (especially medically) important species from ones as simple as hydrogen cations to those as complex as specific DNA fragments. We present a SERS analysis of species whose characterization is important to our understanding of various mechanisms in the human body and to show its potential as an alternative for methods routinely used in diagnostics and clinics. Furthermore, we explain how such SERS-based sensors operate and point out future prospects in this field.

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Surface enhanced Raman spectroscopy of RNA samples extracted from blood of hepatitis C patients for quantification of viral loads

Cited by 28 publications

References 80 publications

Understanding viruses and viral infections by biophotonic methods

Understanding viruses and viral infections by biophotonic methods

Contributions of vibrational spectroscopy to virology: A review

Applications of Surface-Enhanced Raman Scattering in Biochemical and Medical Analysis

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