Surface‐enhanced Raman spectroscopy of genomic DNA from in vitro grown plant species

Abstract

Surface-enhanced Raman scattering (SERS) effect was used to demonstrate ultrasensitive optical detection of nucleic acids. In this work, the SERS spectra of seven genomic DNAs from leaves of Arnica montana (L.), Fam. Compositae, Astragalus peterfii (Jáv.), Fam. Fabaceae, Kalanchoe x hybrida, Fam. Crassulaceae, strawberry (Fragaria x ananassa Duch.), Fam. Rosaceae, carnation (Dianthus caryophyllus L.), Fam. Caryophyllaceae, apple (Malus domestica Borkh.), Fam. Rosaceae and Persian violet (Exacum affine Balf.), Fam. Gentianaceae were analyzed in the wavenumber range 200-1800 cm −1 . SERS signatures, spectroscopic band assignments and structural interpretations of these plant genomic DNAs are reported. SERS spectra of nucleic acids are compared here with caution, because these signals are time-dependent. The SERS spectra corresponding to DNA from Arnica, Dianthus, Fragaria and Kalanchoe leaves show well-resolved, accurate bands, providing thus a high molecular structural information content. Based on this work, specific plant DNA-ligand interactions or DNA structural changes induced by plant stress conditions associated with their natural environment might be further investigated using SERS spectroscopy. Besides, this study will generate information that is valuable in the development of label-free DNA-based nanosensors for chemical probing in the living cell.

The influence of divalent metal ions on low pH induced LacDNA structural changes as probed with UV resonance Raman spectroscopy

“…UV resonance Raman wavenumbers (cm À1 ) and tentative assignments of LacDNA complexes in different physico-chemical conditions, of the corresponding single-stranded oligonucleotides and of the proper sum of spectra of single oligonucleotides. [1,5,11,19,20,[23][24][25][26][27][28][29][30][31][32] Raman spectra were excited with a 275 nm laser line. The spectral resolution was appreciated to be 3 cm Intensity changes can also give insight into changes in coupling or electronic structure.…”

“…The prominent line near 1385 cm À1 is attributed to dA and dT residues. [23,26,30] The band at 1431 cm À1 , which experiences medium to little resonance enhancement, is assigned to the deoxyribosyl ([Refs. 25,28] and references therein).…”

“…Wavenumber positions of the major peaks are presented in Figs 3 and 4, respectively, being in accordance with those given previously in the literature. [1,5,11,19,20,[23][24][25][26][27][28][29][30][31][32] Table 1 presents a detailed comparative analysis of the UV (275 nm) resonance Raman markers of the six LacDNA complexes, the corresponding single-stranded oligonucleotides (SS1 and SS2) and the proper sum of spectra of single oligonucleotides. Proposed UVRR band assignments found in the literature for similar compounds are also included.…”

Surface-enhanced Raman spectroscopy of genomic DNA from in vitro grown tomato (Lycopersicon esculentum Mill.) cultivars before and after plant cryopreservation

“…SERS signatures, spectroscopic band assignments and structural interpretations for these plant genomic DNAs are reported. Applying an ultrasensitive Raman method for these low level DNA samples became a demand [6,8].…”

“…Particularly, DNA SERS signatures might provide an objective evaluation of genetic identity of plants based on species, cultivars or geographic origin [6,7].…”