Surface‐enhanced Raman spectroscopy study of the interaction of the antitumoral drug emodin with human serum albumin

Fabriciová, Gabriela; Sánchez-Cortés, Santiago; Garcia-Ramos, J.V.; Miškovský, Pavol

doi:10.1002/bip.20058

Cited by 51 publications

(44 citation statements)

References 19 publications

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“…In addition, the interaction of FA through another primary binding site, IB, may favour the interaction of the drug with site I, since this union induces a relative rotation of domains I and II of HSA which leaves this site more accessible for the union with drugs. 20 Similar results were found for the related molecule emodin, 22 indicating that the dihydroxyanthraquinone drugs exhibit similar binding properties with respect to albumins irrespective of the distribution of the OH groups.…”

Section: Discussionsupporting

confidence: 68%

Joint application of micro‐Raman and surface‐enhanced Raman spectroscopy to the interaction study of the antitumoral anthraquinone drugs danthron and quinizarin with albumins

Fabriciová

Sánchez-Cortés

Garcia-Ramos

et al. 2004

J Raman Spectroscopy

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We report on the joint application of surface-enhanced Raman scattering (SERS) and micro-Raman techniques to the study of danthron (DT) and quinizarin (QZ) and their complexes with human and bovine albumins. We propose a novel method based on the combination of SERS and micro-Raman spectroscopy by using immobilized Ag nanoparticles, which we have named micro-SERS, to improve the sensitivity of the SERS technique and that could be applied in recognition processes of drugs with large biomolecules. The micro-SERS technique demonstrated clear advantages over direct measurements in air: reproducibility, rapidity and high sensitivity due to a large coverage of the metal surface and good organization of the adsorbate on the surface. Furthermore, the chemical damage of the analyte induced by laser irradiation is substantially reduced. By means of this method, we were able to deduce important information concerning the binding properties of DT and QZ when interacting with human and bovine albumins. For instance, the interaction mechanism can be drastically modified when fatty acids are present in albumins, there are large differences between human and bovine albumins in the binding of the studied drugs related to changes in the amino-acid sequence of the binding pockets of these proteins and the interaction mechanism of the analysed drugs can be different depending on their structure.

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Section: Discussionsupporting

confidence: 68%

Joint application of micro‐Raman and surface‐enhanced Raman spectroscopy to the interaction study of the antitumoral anthraquinone drugs danthron and quinizarin with albumins

Fabriciová

Sánchez-Cortés

Garcia-Ramos

et al. 2004

J Raman Spectroscopy

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“…Finally, another interesting area of research is the use of SERS for the study of how various drugs interact with biological molecules and environments. The interaction of various antitumoral drugs [17,18] with proteins was studied using SERS, which helped elucidate the binding mechanisms. Likewise the interaction of doxorubicin with its DNA complex [19] and ethidium derivatives with G-Quadruplex DNA [20] were studied by SERS to reveal specific information about binding processes and the structure of the complexes.…”

Section: Sers For Small Molecule Detection and Analysismentioning

confidence: 99%

Surface enhanced Raman scattering for narcotic detection and applications to chemical biology

Ryder

2005

Current Opinion in Chemical Biology

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Raman spectroscopy is rapidly finding favour for applications in the life science due to the ease with which it can be used to extract significant data from tissue and cells. However, the Raman effect is an inherently weak effect, which hinders the analysis of low concentration analytes. The Raman sensitivity can be improved via the Surface Enhanced Raman Scattering (SERS) effect. In SERS, Raman spectra are dramatically increased when a molecule is adsorbed onto nanoroughened noble metal surfaces such as silver and gold. The degree of enhancement enables single molecule detection, which offers the potential for the unambiguous identification of analytes at concentrations that are useful in both a forensic and a chemical biology context. Here we discuss some of the practical applications of SERS to both low-level narcotic detection, and how this can be applied to chemical biology.

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“…27,28 The metal colloids, as stated previously, have peak absorption in the blue wavelength range. Alternatively, previous studies of SERS and emodin utilized blue-green wavelengths for excitation to achieve highly enhanced spectra.…”

Section: Resultsmentioning

confidence: 98%

Simple Route for Preparing Optically Trappable Probes for Surface-Enhanced Raman Scattering

et al. 2009

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Surface-enhanced Raman scattering (SERS) has proven to be a powerful technique for a wide range of topics such as chemical analysis, materials research, and biological imaging. In this work, we report in detail on the development and characterization of micrometer-sized dielectric beads with metal colloids attached to their surface. The metalized beads were sufficiently transparent enabling optical trapping while the presence of metal islands provided the SERS. This method is fast and simple and is void of complications when compared to the Tollen's test used in previous publications. This highly efficient probe can be placed and scanned with nanometric accuracy near living cells. The process to create such probes is described including discussion of various parameters that are critical in achieving the desired results. Additionally, their use is demonstrated by detecting low quantities of a drug in aqueous solution and in a cell membrane.The enhancement of local field intensity due to local plasmon resonances near nanoscopically textured metal structures leads to many fascinating optical effects. One of them is the surfaceenhanced Raman scattering (SERS), which has been established as a powerful spectroscopic technique to chemical analysis combining high sensitivity with structural information. 1 A great deal of efforts has been made toward developing SERS substrates over the past decade. 2 Nanoparticles (NPs) with sizes around tens of nanometers have an appeal due to their larger scattering cross sections and when they are closely associated (<10 nm) intense fields emanate and contribute to the high sensitivity of the technique. Early works in developing suitable SERS surfaces concentrated on evaporation and thermal annealing of the metal to surfaces. 3,4 At a later stage, 3D hybrid structures of metal NPs on dielectric beads 5 were developed in what are now well known as nanoshells, and their applications toward SERS were initially demonstrated. 6 A simple and robust SERS scanning system can be developed with the use of optical tweezers. 7 By optically trapping a SERS active probe, advantages are gained from the combination of the high enhancement from a static configuration between the laser focus and metal surface and the scanning ability due to the in situ trapping, so that this highly efficient probe can be placed and scanned with nanometric accuracy near, for instance, living cells. However, for nanometer-sized particles, times for trapping in 3D are short. 8 For metal particles of this size, the scattering force exerted by the trapping beam overcomes the gradient force in the propagation direction.In 2002, the Tollen's test was used to create silver islands on dielectric beads and compared these surfaces to the original method. 9 The silvered beads were sufficiently transparent to allow light to refract through them, enabling the optical trapping while the presence of metal islands provided the SERS. 10 The beads made by Tollen's test 10,11 were partially covered with silver, but the homogeneity and dist...

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Surface‐enhanced Raman spectroscopy study of the interaction of the antitumoral drug emodin with human serum albumin

Cited by 51 publications

References 19 publications

Joint application of micro‐Raman and surface‐enhanced Raman spectroscopy to the interaction study of the antitumoral anthraquinone drugs danthron and quinizarin with albumins

Joint application of micro‐Raman and surface‐enhanced Raman spectroscopy to the interaction study of the antitumoral anthraquinone drugs danthron and quinizarin with albumins

Surface enhanced Raman scattering for narcotic detection and applications to chemical biology

Simple Route for Preparing Optically Trappable Probes for Surface-Enhanced Raman Scattering

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