Surface-exposed chaperonin 60 derived from Propionibacterium freudenreichii MJ2 inhibits adipogenesis by decreasing the expression of C/EBPα/PPARγ

An, Mirae; Lim, Young-Hee

doi:10.1038/s41598-023-46436-x

Cited by 3 publications

(1 citation statement)

References 40 publications

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“…A large number of different biological moonlighting functions have been ascribed to chaperonin 60 (Hsp60) proteins (46). Notably, a recent study revealed antiadipogenic activity of surface-exposed Hsp60 in P. freudenreichii strain MJ2 (47). For Helicobacter pylori Hsp60, extracellular properties include iron binding (48), an ability that could be beneficial in aerobic conditions, thus explaining differential surface abundance in response to atmospheric conditions for PFR .…”

Section: Resultsmentioning

confidence: 99%

Aerobic Adaptation and Metabolic Dynamics ofPropionibacterium freudenreichiiDSM 20271: Insights from Comparative Transcriptomics and Surfaceome Analysis

Loivamaa,

Sillanpää,

Deptula

et al. 2024

Preprint

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Propionibacterium freudenreichii(PFR) DSM 20271 is a bacterium known for its ability to thrive in diverse environments and to produce vitamin B12. Despite its anaerobic preference, recent studies have elucidated its ability to prosper in the presence of oxygen, prompting a deeper exploration of its physiology under aerobic conditions. Here, we investigated the response of DSM 20271 to aerobic growth by employing comparative transcriptomic and surfaceome analyses alongside metabolite profiling. Cultivation under controlled partial pressure of oxygen (pO2) conditions revealed significant increases in biomass formation and altered metabolite production, notably of B12 vitamin, pseudovitamin-B12, propionate and acetate, under aerobic conditions. Transcriptomic analysis identified differential expression of genes involved in lactate metabolism, TCA cycle, and electron transport chain, suggesting metabolic adjustments to aerobic environments. Moreover, surfaceome analysis unveiled growth environment-dependent changes in surface protein abundance, with implications for sensing and adaptation to atmospheric conditions. Supplementation experiments with key compounds highlighted the potential for enhancing aerobic growth, emphasizing the importance of iron and α-ketoglutarate availability. Furthermore, in liquid culture, FeSO4supplementation led to increased heme production and reduced vitamin B12 production, highlighting the impact of oxygen and iron availability on the metabolic pathways. These findings deepen our understanding ofPFR's physiological responses to oxygen availability and offer insights for optimizing its growth in industrial applications.

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Section: Resultsmentioning

confidence: 99%

Aerobic Adaptation and Metabolic Dynamics ofPropionibacterium freudenreichiiDSM 20271: Insights from Comparative Transcriptomics and Surfaceome Analysis

Loivamaa,

Sillanpää,

Deptula

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Aerobic adaptation and metabolic dynamics of Propionibacterium freudenreichii DSM 20271: insights from comparative transcriptomics and surfaceome analysis

Loivamaa,

Sillanpää,

Deptula

et al. 2024

mSystems

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Propionibacterium freudenreichii ( PFR ) DSM 20271 T is a bacterium known for its ability to thrive in diverse environments and to produce vitamin B12. Despite its anaerobic preference, recent studies have elucidated its ability to prosper in the presence of oxygen, prompting a deeper exploration of its physiology under aerobic conditions. Here, we investigated the response of DSM 20271 T to aerobic growth by employing comparative transcriptomic and surfaceome analyses alongside metabolite profiling. Cultivation under controlled partial pressure of oxygen (pO 2 ) conditions revealed significant increases in biomass formation and altered metabolite production, notably of vitamin B12, pseudovitamin-B12, propionate, and acetate, under aerobic conditions. Transcriptomic analysis identified differential expression of genes involved in lactate metabolism, tricarboxylic acid cycle, and electron transport chain, suggesting metabolic adjustments to aerobic environments. Moreover, surfaceome analysis unveiled growth environment-dependent changes in surface protein abundance, with implications for adaptation to atmospheric conditions. Supplementation experiments with key compounds highlighted the potential for enhancing aerobic growth, emphasizing the importance of iron and α-ketoglutarate availability. Furthermore, in liquid culture, FeSO 4 supplementation led to increased heme production and reduced vitamin B12 production, highlighting the impact of oxygen and iron availability on the metabolic pathways. These findings deepen our understanding of PFR ’s physiological responses to oxygen availability and offer insights for optimizing its growth in industrial applications. IMPORTANCE The study of the response of Propionibacterium freudenreichii to aerobic growth is crucial for understanding how this bacterium adapts to different environments and produces essential compounds like vitamin B12. By investigating its physiological changes under aerobic conditions, we can gain insights into its metabolic adjustments and potential for enhanced growth. These findings not only deepen our understanding of P. freudenreichii' s responses to oxygen availability but also offer valuable information for optimizing its growth in industrial applications. This research sheds light on the adaptive mechanisms of this bacterium, providing a foundation for further exploration and potential applications in various fields.

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Anti-Obesity Effect of Aster yomena Extracts through Regulation of AMPK/SIRT1 Signaling

Hwang,

et al. 2024

JKFN

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In this study, the anti-obesity effect of a 50% ethanol extract from Aster yomena (AY), a natural material with various physiological activities, including anti-inflammatory and anti-allergic effects, was investigated in C3H/ 10T1/2 cells. The AY extract (10, 200, or 500 μg/mL) suppressed both the accumulation of lipid droplets, as stained with Oil Red O, and the production of intracellular triglycerides. The extract also suppressed factors related to adipogenesis (CCAAT/enhancer-binding protein alpha, beta, and peroxisome proliferator-activated receptor gamma; PPARγ) and lipogenesis (sterol-regulatory element binding protein-1c, fatty acid synthase, and fatty acid binding protein 4), but it enhanced the expressions of β-oxidation-related mRNA and proteins (AMP-activated protein kinase, acetyl-CoA carboxylase, PPARγ coactivator-1 alpha, and carnitine palmitoyltransferase-1 alpha). In addition, the extract dose-dependently regulated leptin and adiponectin levels. These results suggest the anti-obesity effect of the AY extract is caused by its targeting the above-mentioned adipogenic and β-oxidation factors and that it has potential use for treating obesity.

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Surface-exposed chaperonin 60 derived from Propionibacterium freudenreichii MJ2 inhibits adipogenesis by decreasing the expression of C/EBPα/PPARγ

Cited by 3 publications

References 40 publications

Aerobic Adaptation and Metabolic Dynamics ofPropionibacterium freudenreichiiDSM 20271: Insights from Comparative Transcriptomics and Surfaceome Analysis

Aerobic Adaptation and Metabolic Dynamics ofPropionibacterium freudenreichiiDSM 20271: Insights from Comparative Transcriptomics and Surfaceome Analysis

Aerobic adaptation and metabolic dynamics of Propionibacterium freudenreichii DSM 20271: insights from comparative transcriptomics and surfaceome analysis

Anti-Obesity Effect of Aster yomena Extracts through Regulation of AMPK/SIRT1 Signaling

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