Surface-fill H2S-releasing silk fibroin hydrogel for brain repair through the repression of neuronal pyroptosis

Chen, Xueshi; Huang, Xinqi; Liu, Chao; Li, Sunao; Yang, Zhenbei; Zhang, Feng; Chen, Xiping; Shan, Haiyan; Tao, Luyang; Zhang, Mingyang

doi:10.1016/j.actbio.2022.11.021

Cited by 28 publications

(12 citation statements)

References 115 publications

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“…The TBI model had the following parameters: high pressure of 200 Kpa, and depth of 1.0 mm. This model is referred to as a mild-TBI model in the literature [ 41 ]. Finally, the scalp was sutured with 3/0 silk and the wound was cleaned again with 10% povidone-iodine.…”

Section: Methodsmentioning

confidence: 99%

The Role of Apoptosis and Autophagy in the Hypothalamic-Pituitary-Adrenal (HPA) Axis after Traumatic Brain Injury (TBI)

Taheri

Karaca

Mehmetbeyoglu

et al. 2022

IJMS

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Traumatic brain injury (TBI) is a major health problem affecting millions of people worldwide and leading to death or permanent damage. TBI affects the hypothalamic–pituitary–adrenal (HPA) axis either by primary injury to the hypothalamic–hypophyseal region or by secondary vascular damage, brain, and/or pituitary edema, vasospasm, and inflammation. Neuroendocrine dysfunctions after TBI have been clinically described in all hypothalamic–pituitary axes. We established a mild TBI (mTBI) in rats by using the controlled cortical impact (CCI) model. The hypothalamus, pituitary, and adrenals were collected in the acute (24 h) and chronic (30 days) groups after TBI, and we investigated transcripts and protein-related autophagy (Lc3, Bcln1, P150, Ulk, and Atg5) and apoptosis (pro-caspase-3, cleaved caspase-3). Transcripts related to autophagy were reduced in the hypothalamus, pituitary, and adrenals after TBI, however, this was not reflected in autophagy-related protein levels. In contrast, protein markers related to apoptosis increased in the adrenals during the acute phase and in the pituitary during the chronic phase. TBI stresses induce a variation of autophagy-related transcripts without modifying the levels of their proteins in the HPA axis. In contrast, protein markers related to apoptosis are increased in the acute phase in the adrenals, which could lead to impaired communication via the hypothalamus, pituitary, and adrenals. This may then explain the permanent pituitary damage with increased apoptosis and inflammation in the chronic phase. These results contribute to the elucidation of the mechanisms underlying endocrine dysfunctions such as pituitary and adrenal insufficiency that occur after TBI. Although the adrenals are not directly affected by TBI, we suggest that the role of the adrenals along with the hypothalamus and pituitary should not be ignored in the acute phase after TBI.

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Section: Methodsmentioning

confidence: 99%

The Role of Apoptosis and Autophagy in the Hypothalamic-Pituitary-Adrenal (HPA) Axis after Traumatic Brain Injury (TBI)

Taheri

Karaca

Mehmetbeyoglu

et al. 2022

IJMS

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“…We estimated Garcia scoring system, and performed morris water maze test, Beam balance, and Rotarod test as previous study described [21][22][23][24]. Garcia scoring system was used to grade the severity of SAH on day 1.…”

Section: Neurological Assessmentmentioning

confidence: 99%

Overexpression of Heme oxygenase 1 enhances the neuroprotective effects of exosomes in subarachnoid hemorrhage by suppressing oxidative stress and endoplasmic reticulum stress

Gao,

Su,

Pang

et al. 2024

Preprint

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Aims: To investigate the therapeutic effects and potential mechanism of exosomes from Heme oxygenase 1 (HO-1)-overexpressing human umbilical cord mesenchymal stem cells (ExoHO-1) on subarachnoid hemorrhage (SAH) mice. Methods: Western blotting, particles analyzer, and transmission electron microscopy were used to identify the exosomes. Garcia scoring system, Beam balance, Rotarod test, and Morris water maze test were performed to assessed the effect of ExoHO-1 and ExoCtrl on neurological function of SAH mice. TUNEL and Nissl staining were used to examinate the neuron apoptosis. Immunofluorescence, Western blotting, DHE, Enzyme-linked immunosorbent assay, and commercial kits were used to examine the levels of oxidative stress and endoplasmic reticulum stress. Results: HO-1-overexpressing human umbilical cord mesenchymal stem cells loaded HO-1 into their exosomes. ExoHO-1 exhibited a significantly beneficial effects on short-term and long-term neurological function protecting. By reducing activation of PERK/CHOP/Caspase12 pathway and levels of oxidative stress, ExoHO-1 more effectively inhibited neuronal apoptosis in ipsilateral temporal cortex. Conclusion: HO-1 over-expression enhanced the therapy of exosomes on the SAH mice by against neuronal apoptosis in SAH. These therapeutic effects are likely through suppressing the oxidative stress and endoplasmic reticulum stress.

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“…A surface‐filled H 2 S‐releasing silk fibroin hydrogel (H 2 S@SF gel) was synthesized to achieve the slow release of H 2 S, rebuilding H 2 S endogenous homeostasis in TBI ( Figure 7 ). [ 108 ] The exogenous H 2 S reduces neuronal death, and shows neuroprotective effect through attenuating neuroinflammation. [ 109,110 ] However, conventional administration needs large doses through blood circulation, causing toxic side effect to other tissues.…”

Section: Biomaterials For Regulating Neuroinflammationmentioning

confidence: 99%

“…[104,107] SF has a good property to regulate inflammation through NF-𝜅B signaling pathway. A surface-filled H 2 Sreleasing silk fibroin hydrogel (H 2 S@SF gel) was synthesized [108] Copyright 2022, Elsevier.…”

Section: Regulation Of Immunocyte Activation and Infiltrationmentioning

confidence: 99%

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Recent Advances in Biomaterials‐Based Therapies for Alleviation and Regeneration of Traumatic Brain Injury

Chen

Zhao

et al. 2023

Macromolecular Bioscience

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Traumatic brain injury (TBI), a major public health problem accompanied with numerous complications, usually leads to serve disability and huge financial burden. The adverse and unfavorable pathological environment triggers a series of secondary injuries, resulting in serious loss of nerve function and huge obstacle of endogenous nerve regeneration. With the advances in adaptive tissue regeneration biomaterials, regulation of detrimental microenvironment to reduce the secondary injury and to promote the neurogenesis becomes possible. The adaptive biomaterials could respond and regulate biochemical, cellular, and physiological events in the secondary injury, including excitotoxicity, oxidative stress, and neuroinflammation, to rebuild circumstances suitable for regeneration. In this review, the development of pathology after TBI is discussed, followed by the introduction of adaptive biomaterials based on various pathological characteristics. The adaptive biomaterials carried with neurotrophic factors and stem cells for TBI treatment are then summarized. Finally, the current drawbacks and future perspective of biomaterials for TBI treatment are suggested.

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Surface-fill H2S-releasing silk fibroin hydrogel for brain repair through the repression of neuronal pyroptosis

Cited by 28 publications

References 115 publications

The Role of Apoptosis and Autophagy in the Hypothalamic-Pituitary-Adrenal (HPA) Axis after Traumatic Brain Injury (TBI)

The Role of Apoptosis and Autophagy in the Hypothalamic-Pituitary-Adrenal (HPA) Axis after Traumatic Brain Injury (TBI)

Overexpression of Heme oxygenase 1 enhances the neuroprotective effects of exosomes in subarachnoid hemorrhage by suppressing oxidative stress and endoplasmic reticulum stress

Recent Advances in Biomaterials‐Based Therapies for Alleviation and Regeneration of Traumatic Brain Injury

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