Surface functionalisation-dependent adverse effects of metal nanoparticles and nanoplastics in zebrafish embryos

Hansjosten, Iris; Takamiya, Masanari; Rapp, J.; Reiner, Luisa; Fritsch‐Decker, Susanne; Mattern, Dorit; Andraschko, Silvia; Anders, Chantal; Pace, Giuseppina; Dickmeis, Thomas; Peravali, Ravindra; Rastegar, Sepand; Strähle, Uwe; Hsiao, I‐Lun; Gilliland, Douglas; Ojea-Jiménez, Isaac; Ambrose, Selina V. Y.; Belinga-Desaunay-Nault, Marie-France A.; Khan, Abdullah O.; Lynch, Iseult; Valsami‐Jones, Eugenia; Diabaté, Silvia; Weiss, Carsten

doi:10.1039/d1en00299f

Cited by 15 publications

(12 citation statements)

References 103 publications

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“…65 This mechanism can differ with a variety of chemical properties of functionalized agents and the employed concentration of the incubated nanocarriers. 66 Therefore, we emphasize that the data reported herein requires further study of the tested zebrafish embryo stage to obtain more information on its hatching profile.…”

Section: ■ Results and Discussionmentioning

confidence: 94%

“…There have also been reports that functionalized nanocarriers can also interfere with the hatching process by inhibiting the enzyme or by slowing the maturation of the hatching gland . This mechanism can differ with a variety of chemical properties of functionalized agents and the employed concentration of the incubated nanocarriers . Therefore, we emphasize that the data reported herein requires further study of the tested zebrafish embryo stage to obtain more information on its hatching profile.…”

Section: Resultsmentioning

confidence: 99%

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Dissolution and Biological Assessment of Cancer-Targeting Nano-ZIF-8 in Zebrafish Embryos

Kamal

Abdulmalek

Fakurazi

et al. 2022

ACS Biomater. Sci. Eng.

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Cancer-targeting nanotherapeutics offer promising opportunities for selective delivery of cytotoxic chemotherapeutics to cancer cells. However, the understanding of dissolution behavior and safety profiles of such nanotherapeutics is scarce. In this study, we report the dissolution profile of a cancer-targeting nanotherapeutic, gemcitabine (GEM) encapsulated within RGD-functionalized zeolitic imidazolate framework-8 (GEM⊂RGD@nZIF-8), in dissolution media having pH = 6.0 and 7.4. GEM⊂RGD@nZIF-8 was not only responsive in acidic media (pH = 6.0) but also able to sustain the dissolution rate (57.6%) after 48 h compared to non-targeting nanotherapeutic GEM⊂nZIF-8 (76%). This was reflected by the f 2 value of 36.1, which indicated a difference in the dissolution behaviors of GEM⊂RGD@nZIF-8 and GEM⊂nZIF-8 in acidic media compared to those in neutral media (pH = 7.4). A dissolution kinetic study showed that the GEM release mechanism from GEM⊂RGD@nZIF-8 followed the Higuchi model. In comparison to a non-targeting nanotherapeutic, the cancer-targeting nanotherapeutic exhibited an enhanced permeability rate in healthy zebrafish embryos but did not induce lethality to 50% of the embryos (LC50 > 250 μg mL–1) with significantly improved survivability (75%) after 96 h of incubation. Monitoring malformation showed minimal adverse effects with only 8.3% of edema at 62.5 μg mL–1. This study indicates that cancer-targeting GEM⊂RGD@nZIF, with its pH-responsive behavior for sustaining chemotherapeutic dissolution in a physiologically relevant environment and its non-toxicity toward the healthy embryos within the tested concentrations, has considerable potential for use in cancer treatment.

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Section: ■ Results and Discussionmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Dissolution and Biological Assessment of Cancer-Targeting Nano-ZIF-8 in Zebrafish Embryos

Kamal

Abdulmalek

Fakurazi

et al. 2022

ACS Biomater. Sci. Eng.

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“…However, excessive free Zn ions are toxic and may be bound by Zn-binding proteins, such as metalloproteins [41]. The dissolved Zn ions interfere with embryo hatching through a chelator-sensitive mechanism that involves the ligation of histidines in the metalloprotease ZHE1, which is responsible for degradation of the chorionic membrane [42,43]. The effects of ZnONPs on delaying hatching were attenuated in filtered natural water samples compared with MQ water, suggesting the slow release of Zn ions from ZnONPs and the interaction of dissolved Zn ions with the complex components in the water environment, which subsequently mitigate effects on embryonic development.…”

Section: Discussionmentioning

confidence: 99%

Toxic Effects and Mechanisms of Silver and Zinc Oxide Nanoparticles on Zebrafish Embryos in Aquatic Ecosystems

et al. 2022

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The global application of engineered nanomaterials and nanoparticles (ENPs) in commercial products, industry, and medical fields has raised some concerns about their safety. These nanoparticles may gain access into rivers and marine environments through industrial or household wastewater discharge and thereby affect the ecosystem. In this study, we investigated the effects of silver nanoparticles (AgNPs) and zinc oxide nanoparticles (ZnONPs) on zebrafish embryos in aquatic environments. We aimed to characterize the AgNP and ZnONP aggregates in natural waters, such as lakes, reservoirs, and rivers, and to determine whether they are toxic to developing zebrafish embryos. Different toxic effects and mechanisms were investigated by measuring the survival rate, hatching rate, body length, reactive oxidative stress (ROS) level, apoptosis, and autophagy. Spiking AgNPs or ZnONPs into natural water samples led to significant acute toxicity to zebrafish embryos, whereas the level of acute toxicity was relatively low when compared to Milli-Q (MQ) water, indicating the interaction and transformation of AgNPs or ZnONPs with complex components in a water environment that led to reduced toxicity. ZnONPs, but not AgNPs, triggered a significant delay of embryo hatching. Zebrafish embryos exposed to filtered natural water spiked with AgNPs or ZnONPs exhibited increased ROS levels, apoptosis, and lysosomal activity, an indicator of autophagy. Since autophagy is considered as an early indicator of ENP interactions with cells and has been recognized as an important mechanism of ENP-induced toxicity, developing a transgenic zebrafish system to detect ENP-induced autophagy may be an ideal strategy for predicting possible ecotoxicity that can be applied in the future for the risk assessment of ENPs.

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“…The surface of plastics can be engineered with different moieties to improve their stability [ 107 ], adhesion [ 108 ] and biocompatibility [ 109 ]. Debris of these functionalized plastics may preserve the engineered functionalities and display different surface charges [ 110 ].…”

Section: Matrices and Physicochemical Properties Of Environmental Mic...mentioning

confidence: 99%

Coronas of micro/nano plastics: a key determinant in their risk assessments

et al. 2022

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As an emerging pollutant in the life cycle of plastic products, micro/nanoplastics (M/NPs) are increasingly being released into the natural environment. Substantial concerns have been raised regarding the environmental and health impacts of M/NPs. Although diverse M/NPs have been detected in natural environment, most of them display two similar features, i.e.,high surface area and strong binding affinity, which enable extensive interactions between M/NPs and surrounding substances. This results in the formation of coronas, including eco-coronas and bio-coronas, on the plastic surface in different media. In real exposure scenarios, corona formation on M/NPs is inevitable and often displays variable and complex structures. The surface coronas have been found to impact the transportation, uptake, distribution, biotransformation and toxicity of particulates. Different from conventional toxins, packages on M/NPs rather than bare particles are more dangerous. We, therefore, recommend seriously consideration of the role of surface coronas in safety assessments. This review summarizes recent progress on the eco–coronas and bio-coronas of M/NPs, and further discusses the analytical methods to interpret corona structures, highlights the impacts of the corona on toxicity and provides future perspectives.

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Surface functionalisation-dependent adverse effects of metal nanoparticles and nanoplastics in zebrafish embryos

Abstract: High throughput imaging is used to assess hatching, lethality and malformations in zebrafish embryos and is suitable for hazard ranking of different nanomaterials.

Cited by 15 publications

References 103 publications

Dissolution and Biological Assessment of Cancer-Targeting Nano-ZIF-8 in Zebrafish Embryos

Dissolution and Biological Assessment of Cancer-Targeting Nano-ZIF-8 in Zebrafish Embryos

Toxic Effects and Mechanisms of Silver and Zinc Oxide Nanoparticles on Zebrafish Embryos in Aquatic Ecosystems

Coronas of micro/nano plastics: a key determinant in their risk assessments

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