Surface functionalization for spore-based biosensors with organosilanes

Arreola, Julio; Oberländer, Jan; Mätzkow, Malte; Keusgen, Michael; Schöning, Michael J.

doi:10.1016/j.electacta.2017.04.157

Cited by 11 publications

(11 citation statements)

References 26 publications

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“…The reference channel measures the impedance of one IDE structure to serve as an indicator to possible changes to sensor materials by the applied chemical sterilization protocol. During data analysis, there has been no noted changes to the impedance value of the IDE reference structure as noted in our previous studies . All sensor states have been independently characterized by impedance spectroscopy to track changes in the impedance value.…”

Section: Methodsmentioning

confidence: 96%

“…The impedimetric biosensor chip has been developed and characterized in previous works . A planar IDE structure has been designed as follows: 307 electrode pairs each with a length of 3.25 mm; the width of one electrode finger is 4 μm with an interspacing of 6 μm (as depicted in Figure a).…”

Section: Methodsmentioning

confidence: 99%

“…The sensors have been in‐house fabricated on a 500 μm thick Borofloat 33 ® glass wafer (Schott AG, Germany) serving as the sensor substrate by means of standard photolithography and thin‐film processing steps . The thin‐film electrode materials, 100 nm gold on top of 10 nm chromium were deposited in an e‐beam evaporation device (Leybold GmbH, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…An impedimetric biosensor based on interdigitated electrode (IDE) structures has been developed by the authors in previous work . A test microorganism (spores of Bacillus atrophaeus taken from a spore suspension based on deionized (DI)‐water) has been immobilized on the IDE structure and characterized in respect to the H 2 O 2 sterilization process.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Experimental and Numerical Analyzes of a Sensor Based on Interdigitated Electrodes for Studying Microbiological Alterations

Jildeh

Oberländer

Kirchner

et al. 2018

Physica Status Solidi (a)

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In this work, a cell-based biosensor to evaluate the sterilization efficacy of hydrogen peroxide vapor sterilization processes is characterized. The transducer of the biosensor is based on interdigitated gold electrodes fabricated on an inert glass substrate. Impedance spectroscopy is applied to evaluate the sensor behavior and the alteration of test microorganisms due to the sterilization process. These alterations are related to changes in relative permittivity and electrical conductivity of the bacterial spores. Sensor measurements are conducted with and without bacterial spores (Bacillus atrophaeus), as well as after an industrial sterilization protocol. Equivalent two-dimensional numerical models based on finite element method of the periodic finger structures of the interdigitated gold electrodes are designed and validated using COMSOL 1 Multiphysics software by the application of known dielectric properties. The validated models are used to compute the electrical properties at different sensor states (blank, loaded with spores, and after sterilization). As a final result, we will derive and tabulate the frequencydependent electrical parameters of the spore layer using a novel model that combines experimental data with numerical optimization techniques.

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Section: Methodsmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Experimental and Numerical Analyzes of a Sensor Based on Interdigitated Electrodes for Studying Microbiological Alterations

Jildeh

Oberländer

Kirchner

et al. 2018

Physica Status Solidi (a)

Self Cite

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“…Over the years, many different biomolecules have been covalently anchored on SiO 2 [27]: DNA strands [28,29], enzymes [14,30], and even whole cells [31].…”

Section: Introductionmentioning

confidence: 99%

Functionalization of Bulk SiO2 Surface with Biomolecules for Sensing Applications: Structural and Functional Characterizations

et al. 2018

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Biomolecule immobilization on bulk silicon dioxide (SiO 2 ) is an important aspect in the field of Si-based interfaces for biosensing. The approach used for surface preparation should guarantee not only the stable anchoring of biomolecules but also their structural integrity and biological functioning. In this paper, we review our findings on the SiO 2 functionalization process to immobilize a variety of biomolecules, including glucose oxidase, horseradish peroxide, metallothionein, and DNA molecules. Morphological and chemical characterization of SiO 2 surfaces after biomolecule immobilization using techniques already employed in the microelectronic industry are presented and discussed. Optical and spectrophotometric analysis revealed the preservation of biomolecules' activity once they are anchored on the biointerface.

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Flexible Calorimetric Gas Sensors for Detection of a Broad Concentration Range of Gaseous Hydrogen Peroxide: A Step Forward to Online Monitoring of Food‐Package Sterilization Processes

Vahidpour

Oberländer

Schöning

2018

Physica Status Solidi (a)

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In this study, flexible calorimetric gas sensors are developed for specific detection of gaseous hydrogen peroxide (H 2 O 2 ) over a wide concentration range, which is used in sterilization processes for aseptic packaging industry. The flexibility of these sensors is an advantage for identifying the chemical components of the sterilant on the corners of the food boxes, so-called "cold spots", as critical locations in aseptic packaging, which are of great importance. These sensors are fabricated on flexible polyimide films by means of thin-film technique. Thin layers of titanium and platinum have been deposited on polyimide to define the conductive structures of the sensors. To detect the high-temperature evaporated H 2 O 2 , a differential temperature setup is proposed. The sensors are evaluated in a laboratory-scaled sterilization system to simulate the sterilization process. The concentration range of the evaporated H 2 O 2 from 0 to 7.7% v/v was defined and the sensors have successfully detected high as well as low H 2 O 2 concentrations with a sensitivity of 5.04 C/% v/v. The characterizations of the sensors confirm their precise fabrication, high sensitivity and the novelty of low H 2 O 2 concentration detections for future inline monitoring of food-package sterilization.

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Surface functionalization for spore-based biosensors with organosilanes

Cited by 11 publications

References 26 publications

Experimental and Numerical Analyzes of a Sensor Based on Interdigitated Electrodes for Studying Microbiological Alterations

Experimental and Numerical Analyzes of a Sensor Based on Interdigitated Electrodes for Studying Microbiological Alterations

Functionalization of Bulk SiO2 Surface with Biomolecules for Sensing Applications: Structural and Functional Characterizations

Flexible Calorimetric Gas Sensors for Detection of a Broad Concentration Range of Gaseous Hydrogen Peroxide: A Step Forward to Online Monitoring of Food‐Package Sterilization Processes

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