Surface functionalization of dual growth factor on hydroxyapatite-coated nanofibers for bone tissue engineering

Udomluck, Nopphadol; Lee, Haram; Hong, Seungpyo; Lee, Soo−Hong; Park, Hansoo

doi:10.1016/j.apsusc.2020.146311

Cited by 53 publications

(32 citation statements)

References 65 publications

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“…Electrospun nanofiber mats (ENMs), due to their high surface area, ease of production, fiber network interconnection, biocompatibility, breathability, and resemblance to natural extracellular matrix have been potentially considered for cell culture applications viz cell adhesion, cell migration, and cell viability [ 10 , 11 , 12 , 13 ]. In parallel, ENMs have been utilized in several applications including sensors [ 14 ], wound dressing [ 10 ], drug delivery [ 5 ], tissue engineering [ 15 ], smart apparels [ 16 , 17 ] high performance filters (water, bacteria, virus, heavy metal, and such impurities) [ 18 , 19 , 20 , 21 ]. Depending on the required application, cell adhesion and cell migration behaviors may differ due to the difference in cell size, type, or the culturing surface [ 4 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Poly(Ethylene-glycol 1,4-Cyclohexane Dimethylene-Isosorbide-Terephthalate) Electrospun Nanofiber Mats for Potential Infiltration of Fibroblast Cells

et al. 2021

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Recently, bio-based electrospun nanofiber mats (ENMs) have gained substantial attention for preparing polymer-based biomaterials intended for use in cell culture. Herein, we prepared poly(ethylene-glycol 1,4-Cyclohexane dimethylene-isosorbide-terephthalate) (PEICT) ENMs using the electrospinning technique. Cell adhesion and cell viability of PEICT ENMs were checked by fibroblast cell culture. Field emission electron microscope (FE-SEM) image showed a randomly interconnected fiber network, smooth morphology, and cell adhesion on PEICT ENM. Fibroblasts were cultured in an adopted cell culturing environment on the surface of PEICT ENMs to confirm their biocompatibility and cell viability. Additionally, the chemical structure of PEICT ENM was checked under Fourier-transform infrared (FTIR) spectroscopy and the results were supported by -ray photoelectron (XPS) spectroscopy. The water contact angle (WCA) test showed the hydrophobic behavior of PEICT ENMs in parallel to good fibroblast cell adhesion. Hence, the results confirmed that PEICT ENMs can be potentially utilized as a biomaterial.

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Section: Introductionmentioning

confidence: 99%

Fabrication of Poly(Ethylene-glycol 1,4-Cyclohexane Dimethylene-Isosorbide-Terephthalate) Electrospun Nanofiber Mats for Potential Infiltration of Fibroblast Cells

et al. 2021

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“…The scaffold showed the bioactivity and osteoinduction of rabbit bone marrow-derived MSCs. Udomluck [ 34 ] developed a GF delivery system based on heparin chemically conjugated to decellularized bone particles to allow for electrostatic tethering of PDGF. Bone particles with tethered GF promoted bone mineral deposition by adipose-derived stem cells in vitro and, hence, bone formation mediated by stem cells in vivo within murine critical-sized calvarial defects.…”

Section: Encapsulation Incorporation and Related Delivery Stratementioning

confidence: 99%

“…A combination of angiogenic (VEGF), cell recruiting (platelet-derived growth factor (PDGF)), and osteogenic (BMPs) GFs has been designed and demonstrated a synergistic effect that is more beneficial to bone repair than any GF delivered alone [ 33 ]. This synergism was also demonstrated through the immobilization of FGF-2 and BMP-2 in administered ratios on the surfaces of gelatin nanofibers to promote bone regeneration [ 34 ]. BMPs stimulate the osteogenic and chondrogenic differentiation of mesenchymal cells and play a significant role in structural development throughout the body, having a wide range of functions, including embryogenesis and regulation of cells widely expressed in several tissues [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Advances in Growth Factor Delivery for Bone Tissue Engineering

Oliveira

Nie

Podstawczyk

et al. 2021

IJMS

137

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Shortcomings related to the treatment of bone diseases and consequent tissue regeneration such as transplants have been addressed to some extent by tissue engineering and regenerative medicine. Tissue engineering has promoted structures that can simulate the extracellular matrix and are capable of guiding natural bone repair using signaling molecules to promote osteoinduction and angiogenesis essential in the formation of new bone tissues. Although recent studies on developing novel growth factor delivery systems for bone repair have attracted great attention, taking into account the complexity of the extracellular matrix, scaffolding and growth factors should not be explored independently. Consequently, systems that combine both concepts have great potential to promote the effectiveness of bone regeneration methods. In this review, recent developments in bone regeneration that simultaneously consider scaffolding and growth factors are covered in detail. The main emphasis in this overview is on delivery strategies that employ polymer-based scaffolds for spatiotemporal-controlled delivery of both single and multiple growth factors in bone-regeneration approaches. From clinical applications to creating alternative structural materials, bone tissue engineering has been advancing constantly, and it is relevant to regularly update related topics.

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“…As a versatile technique, electrospinning is used to prepare nanofibers based on varieties of materials for the application of water treatment, filtration, drug loading and catalysis. In addition, the structure of nanofibers is able to mimic the extracellular matrix (ECM) to support cell adhesion and proliferation, which has been widely considered to be excellent in tissue regeneration including bone, vascular, skin and nerve conduit [ 11 , 12 , 13 , 14 , 15 ]. However, due to the weak bioactivity of single component of pure polymer, some bioactive additives are attempted to incorporate with the polymer scaffolds to enhance the corresponding capacity of accelerating tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

The Construction of Multi-Incorporated Polylactic Composite Nanofibrous Scaffold for the Potential Applications in Bone Tissue Regeneration

Nie

Luo

et al. 2021

Nanomaterials

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To improve the bone regeneration ability of pure polymer, varieties of bioactive components were incorporated to a biomolecular scaffold with different structures. In this study, polysilsesquioxane (POSS), pearl powder and dexamethasone loaded porous carbon nanofibers (DEX@PCNFs) were incorporated into polylactic (PLA) nanofibrous scaffold via electrospinning for the application of bone tissue regeneration. The morphology observation showed that the nanofibers were well formed through electrospinning process. The mineralization test of incubation in simulated body fluid (SBF) revealed that POSS incorporated scaffold obtained faster hydroxyapatite depositing ability than pristine PLA nanofibers. Importantly, benefitting from the bioactive components of pearl powder like bone morphogenetic protein (BMP), bone mesenchymal stem cells (BMSCs) cultured on the composite scaffold presented higher proliferation rate. In addition, by further incorporating with DEX@PCNFs, the alkaline phosphatase (ALP) level and calcium deposition were a little higher based on pearl powder. Consequently, the novel POSS, pearl powder and DEX@PCNFs multi-incorporated PLA nanofibrous scaffold can provide better ability to enhance the biocompatibility and accelerate osteogenic differentiation of BMSCs, which has potential applications in bone tissue regeneration.

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Surface functionalization of dual growth factor on hydroxyapatite-coated nanofibers for bone tissue engineering

Cited by 53 publications

References 65 publications

Fabrication of Poly(Ethylene-glycol 1,4-Cyclohexane Dimethylene-Isosorbide-Terephthalate) Electrospun Nanofiber Mats for Potential Infiltration of Fibroblast Cells

Fabrication of Poly(Ethylene-glycol 1,4-Cyclohexane Dimethylene-Isosorbide-Terephthalate) Electrospun Nanofiber Mats for Potential Infiltration of Fibroblast Cells

Advances in Growth Factor Delivery for Bone Tissue Engineering

The Construction of Multi-Incorporated Polylactic Composite Nanofibrous Scaffold for the Potential Applications in Bone Tissue Regeneration

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