Surface-functionalization of isotactic polypropylene via dip-coating with a methacrylate-based terpolymer containing perfluoroalkyl groups and poly(ethylene glycol)

Covalent immobilization of gold nanoparticles on a plastic substrate and subsequent immobilization of biomolecules

“…Immobilization of horseradish peroxidase on AuNPsimmobilized substrate 40 AuNPs-immobilized substrates were washed three times with Milli-Q water and immersed in 2 mL of Milli-Q water containing 5 mM N-hydroxysuccinimide and 10 mM water-soluble carbodiimide and shaken for 30 min at 25 C. The substrates were immersed in phosphate buffer (0.1 M, pH 7.4, 2 mL) containing 0.3 mg mL À1 horseradish peroxidase (HRP) and 0.15 M NaCl and shaken for 2 h at 25 C. The substrates were washed three times with phosphate buffer (0.1 M, pH 7.4) containing 0.05 vol% Tween-20 and 0.15 M NaCl, and then washed twice with Milli-Q water. The enzymatic activity of HRP on the substrate surface was measured with a mixture of 0.1 mL phosphate buffer and a 0.9 mL ELISA-POD substrate kit containing 2,2 0 -azinobis[3-ethylbenzothiazoline-6-sulfonic acid]diammonium salt (ABTS) (Nakarai Tesque, Kyoto, Japan).…”

Design of a Bioinert Interface Using an Amphiphilic Block Copolymer Containing a Bottlebrush Unit of Oligo(oxazoline)

“…Many bioinert polymers have thus far been developed as a coating material that leads to the durability, efficacy, and safety of various articles such as ship bottoms and food packaging. In particular, poly­(ethylene oxide) (PEO), , zwitterionic polymers, , and their derivatives , have been widely used in biorelated fields. Recently, a possible mechanism for how such polymers including poly­(vinyl ether) derivatives and poly­(2-methoxyethyl acrylate) − exhibit such excellent anti-biofouling properties has been proposed on the basis of the interfacial aggregation states and dynamics in water.…”

“…Since most bioinert polymers are hydrophilic or water-soluble, they need to be firmly anchored to solid substrates to avoid their elution into the water phase when they are used to construct the device surface. A typical method for achieving this is the covalent grafting of polymer chains onto the solid based on surface-initiated polymerization or polymer reactions using specific functional groups. ,, Another technique based on the chemical/physical adsorption using appropriate units introduced into a chain has also been reported. , Elsewhere, the surface segregation of functional units exhibiting bioinertness in a matrix polymer has been applied to construct the bioinert water interface. , A simple surface functionalization method with a nonsolvent has also been developed …”

One-Step Biotinylation of Cellulose Paper by Polymer Coating to Prepare a Paper-Based Analytical Device

“…This occurs because of the surface segregation of the hydrophobic moieties in the polymer to minimize the surface energy. [46][47][48][49][50] We previously reported that the surface segregation of PEG moieties during dip-coating with a PEGylated copolymer can be controlled using a perfluoroalkylated surfactant (surfactant 1). 51 In the present study, we aimed to functionalize cellulose paper surfaces by a simple dip-coating procedure using a PEGylated copolymer and surfactant 1.…”

“…Coating with a functional polymer solution is a simple and versatile approach to functionalizing material surfaces and does not require any specific apparatus. − In general, any hydrophilic moieties are likely to be buried in the bulk phase of the polymer when it is used as a coating solution. This occurs because of the surface segregation of the hydrophobic moieties in the polymer to minimize the surface energy. − We previously reported that the surface segregation of PEG moieties during dip-coating with a PEGylated copolymer can be controlled using a perfluoroalkylated surfactant (surfactant 1) …”