Surface Functionalization of Poly(ether imide) Membranes with Linear, Methylated Oligoglycerols for Reducing Thrombogenicity

Lange, M.; Braune, S.; Luetzow, K.; Richau, K.; Scharnagl, Nico; Weinhart, Marie; Neffe, Axel T.; Jung, F.; Haag, Rainer; Lendlein, Andreas

doi:10.1002/marc.201200426

Cited by 41 publications

(43 citation statements)

References 23 publications

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“…thrombocytopenia [88]. More recently, this method was adapted for hemocompatibility screening studies [89] and further modified and automated for thrombogenic materials [90][91][92]. Static test systems simulate low flow or even no flow areas in the vasculature and provide a rapid and sensitive setup to determine the thrombogenicity of a biomaterial, in particular platelet adhesion.…”

Section: Hemocompatibility Testingmentioning

confidence: 99%

Are there sufficient standards for the in vitro hemocompatibility testing of biomaterials?

et al. 2013

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Section: Hemocompatibility Testingmentioning

confidence: 99%

Are there sufficient standards for the in vitro hemocompatibility testing of biomaterials?

et al. 2013

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“…To improve the long-term patency and functionality of small-diameter grafts the luminal surface must be non-thrombogenic, either by surface passivation [1,26,32] and bioactive coating [7,12,20] or endothelialization [25]. Endothelialization in form of prior cell seeding or in vivo recruitment is a completely different encouraging technique for overcoming the limitations of thrombogenicity by creating a natural anti-thrombotic surface on artificial grafts [4].…”

Section: Introductionmentioning

confidence: 99%

Adherence and shear-resistance of primary human endothelial cells on smooth poly(ether imide) films

Schulz

Rüsten-Lange

Krüger

et al. 2014

Clinical Hemorheology and Microcirculation

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BACKGROUND:Occlusions of artificial small-diameter cardiovascular grafts are frequent events after implantation, often caused by clot formations. A main factor is the insufficient hemocompatibility of the inner artificial graft surface, which could be improved by endothelialization. Therefore, one challenge in cardiovascular graft engineering is the establishment of a shear-resistant endothelial cell layer to prevent cell detachment by shear forces after implantation. MATERIALS AND METHODS:Recently, very smooth (R q = 2.37 ± 1.40 nm) poly(ether imide) (PEI) films were introduced as a biocompatible candidate material for cardiovascular devices. In this study the stability of primary human umbilical vein endothelial cell (HUVEC) monolayer was investigated after long-term seeding (nine days) on PEI-films and subsequent exposure to a venous shear stress of 3 dyn/cm² for up to six hours using the cone-and-plate shearing technique. Cell density, growth pattern and morphology of HUVEC were determined prior and after shearing compared to glass as control substrate. HUVEC adhering to the substrate after shear stress were counted and analyzed by fluorescent staining. Supernatants were collected and secretion profile analysis of vasoactive and inflammatory mediators was performed. RESULTS:The cell density on PEI-films compared to the controls was slightly higher after long-term seeding and exposure to shear stress (glass: 71,656 ± 8,830 cells/cm² and 42,239 ± 5,607 cells/cm²; PEI-film: 64,056 ± 2,829 cells/cm 2 and 45,422 ± 2,507 cells/cm 2 before and after shear stress, respectively). Actin-and vinculin-staining revealed a scattered reorganization of the cytoskeleton as well as a formation of stress fibers and focal adhesion points. Secretion of prostacyclin and thromboxane A2 was increased after application of shear stress, but no significant differences were detectable between cells growing on PEI-films or glass. Amounts of secreted inflammatory cytokines IL-6 and IL-8 in the supernatant were significantly lower for HUVEC seeded on PEI-films compared to glass before as well as after stress. CONCLUSION: The study demonstrated that HUVEC were able to resist exposure to venous shear stress when seeded on smooth PEI-films with typical morphology and adhesion behavior. However, HUVEC adherence on PEI was not yet sufficient to retain a complete cell monolayer after shear stress exposure. Occasionally, single cells or cell plaques were disrupted resulting in cell free areas in the confluent HUVEC layer. Apart from this our data suggest that PEI is a suitable substrate for HUVEC under static and dynamic conditions and therefore a promising candidate material for cardiovascular applications. The next objective is a surface functionalization of the PEI-films in a cell specific manner to reach a functionally confluent, shear resistant HUVEC monolayer.

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“…A typical hydrophobic surface such as poly(ether imide)s may be expected to adsorb proteins around an order of magnitude higher [6]. There is not a remarkable difference in protein adhesion among the three proteins investigated and it can be summarized that these polyglycerolbased networks are generally protein resistant.…”

Section: Figure 4 Bgg Adsorption Polyglycerol-based Network Films Wimentioning

confidence: 94%

“…Notably, it has also been presented that hydrophobic surfaces can be functionalized successfully in order to improve protein resistance [6,7]. Recently we have shown a versatile method for preparation of novel polyglycerol based network films for potential biomedical applications [8].…”

Section: Introductionmentioning

confidence: 99%

Quantification of Protein Adsorption on Polyglycerol-based Polymer Network Films

2013

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Neutral, hydrophilic, polymer-based architectures are widely investigated for a wide range of biomedical applications from drug-conjugates to delivery systems and scaffolds for regenerative therapies. In most cases, it is crucial that biomaterials provide a blank, inert background in order to hinder unspecific cell-material interactions so that protein mediated biological events leading to foreign body reactions are prevented. Hydrophilic polyglycerolbased polymer network films are a recently developed class of amorphous macroscopic materials, which offer great versatility in design and control of resultant properties. In this study, protein adsorption on polyglycerol-based polymer network films is investigated by using Micro BCA protein assay for three types of proteins having critical roles in the human body, and various copolymer networks with differing sidechains and crosslink densities.

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Surface Functionalization of Poly(ether imide) Membranes with Linear, Methylated Oligoglycerols for Reducing Thrombogenicity

Cited by 41 publications

References 23 publications

Are there sufficient standards for the in vitro hemocompatibility testing of biomaterials?

Are there sufficient standards for the in vitro hemocompatibility testing of biomaterials?

Adherence and shear-resistance of primary human endothelial cells on smooth poly(ether imide) films

Quantification of Protein Adsorption on Polyglycerol-based Polymer Network Films

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