Surface Functionalization of Thermoplastic Polymers for the Fabrication of Microfluidic Devices by Photoinitiated Grafting

Rohr, Thomas; Ogletree, D. Frank; Švec, František; Fréchet, Jean M. J.

doi:10.1002/adfm.200304229

Cited by 207 publications

(203 citation statements)

References 34 publications

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“…As a result, it often involves the use of a complex porogenic system to form homogeneous polymerization solution, which may complicate the optimization of monolithic structure and often results in poor monolithic structure [32]. Second, monoliths containing ionizable functional groups, such as phosphate group or sulfonic acid group, tend to swell in aqueous buffer due to the solvent effect [33], thus leaving only a small portion of functional groups on the pore surface for desired interactions or binding.…”

Section: Introductionmentioning

confidence: 99%

Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides

Wang

Duan

et al. 2011

J of Separation Science

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To meet the demands of protein phosphorylation study, immobilized zirconium ion affinity chromatography (Zr 41 -IMAC) monolith was prepared by combining UV-initiated polymerization of monolithic support and subsequent photografting in both capillary columns and microchannels. Hydrophilic poly(2-hydroxyethyl methacrylate (HEMA)-coethylene dimethacrylate (EDMA)) monolithic support was prepared under UV irradiation at the wavelength of 365 nm with monomer HEMA, crosslinker EDMA and 2,2-dimethoxy-2-phenylacetophenone as photoinitiator in 1-decanol solution, which provides good biocompatibility and permeability for biomolecule analysis. To introduce chelating ligands, such as phosphate groups, on the pore surface of monolith for metal ion immobilization, photografting of ethylene glycol methacrylate phosphate with benzophenone as the photoinitiator was performed at 254 nm for 300 s. The grafting process and metal ion immobilization can be monitored by measuring the electroosmotic flow produced by the modified monolith, providing a quantitative evaluation of post-modification. This new method for the preparation of Zr 41 -IMAC monolith simplifies the optimization of monolith preparation and avoids the time-consuming chemical modification process. Additionally, advantages include facile preparation in microdevices, easy regenerability and good reproducibility. After optimization, the microchip-based Zr 41 -IMAC monolith was used for phosphopeptide analysis and showed good selectivity in phosphopeptide enrichment with matrix-assisted laser desorption ionization mass spectrometry detection. IntroductionIn recent years, protein phosphorylation has gained much attention because of its significant roles in cellular signal transduction processes [1,2]. However, the amount of phosphorylated proteins in eukaryotic cells often exists in substoichiometry level, which makes the identification of phosphorylation sites challenging [3]. To date, mass spectrometry (MS) analysis has become an effective and commonly used method for characterization of phosphoproteins [4]. To reduce the interference of non-phosphopeptides, selective enrichment strategies, such as immobilized metal ion affinity chromatography (IMAC) [5][6][7], metal oxide affinity chromatography (MOAC) [8,9], and ion exchange chromatography [10][11][12][13], are necessary before MS analysis.Recently, chip-based system has been introduced as a promising platform for phosphopeptide analysis, due to the benefits of low sample amount, high throughput, and easy integration [14]. Several microfluidic devices have been developed by integrating phosphopeptide enrichment with other functions, such as protein alkylation and reduction [15], protein digestion [16], peptide separation and on-line interface for MS instrument [17][18][19][20], which have shown great potentials in the field of phosphoproteome research. However, in these microchip devices, phosphopeptide enrichment components are usually fabricated by packing functionalized particles, such as TiO 2 or IMAC beads, into m...

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Section: Introductionmentioning

confidence: 99%

Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides

Wang

Duan

et al. 2011

J of Separation Science

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“…Photoinitiated grafting and polymerization have also been demonstrated in the confinement of a microchannel. 25,[57][58][59][60] Photografting to thiol-terminated SAMs was used by Besson et al 25 to reduce the required exposure time for patterning wettability gradients in channels. One advantage of grafting is the ability to graft different functionalities without changing the primary surface treatment, as shown by Hu et al 58 Elsewhere, photoinitiated grafting of poly͑acrylamide͒ was used to activate PDMS microchannels for bioconjugation of protein ͑casein͒ to selected regions of the channel.…”

Section: B Photolithographymentioning

confidence: 99%

Surface patterning of bonded microfluidic channels

Priest

2010

Biomicrofluidics

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Microfluidic channels in which multiple chemical and biological processes can be integrated into a single chip have provided a suitable platform for high throughput screening, chemical synthesis, detection, and alike. These microchips generally exhibit a homogeneous surface chemistry, which limits their functionality. Localized surface modification of microchannels can be challenging due to the nonplanar geometries involved. However, chip bonding remains the main hurdle, with many methods involving thermal or plasma treatment that, in most cases, neutralizes the desired chemical functionality. Postbonding modification of microchannels is subject to many limitations, some of which have been recently overcome. Novel techniques include solution-based modification using laminar or capillary flow, while conventional techniques such as photolithography remain popular. Nonetheless, new methods, including localized microplasma treatment, are emerging as effective postbonding alternatives. This Review focuses on postbonding methods for surface patterning of microchannels.

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“…Also, a reliable quality control of the sample is needed prior to the x-ray or neutron experiment. In response to these demands, an experimental setup based on a microfluidic system made from a cyclic olefin polymer ͑COC͒, a thermoplastic polymeric material which is highly transparent for light 21 and x rays of 20 keV, has been presented. 22 The COC based microfluidic chamber 23 is shown in Fig.…”

Section: B Design Of the X-ray Experimentsmentioning

confidence: 99%

Nanostructure of supported lipid bilayers in water

Nickel

2008

Biointerphases

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Biologically functional supported lipid bilayers (SLBs) used in the rising field of nanobiotechnology require fine tuning of the SLB interface with the substrate, e.g., a sensor surface. Depending on the application, membrane functionality implies a homogeneous and dense bilayer and a certain degree of diffusivity in order to allow for a rearrangement in response to, e.g., protein binding. Here, progress in the preparation, characterization, and application of SLBs obtained in the past three to five years are highlighted. Synchrotron techniques, which allow to reveal structural features within the membrane on a length scale of ∼0.5 nm are discussed in more detail, as well as the relation of structural features to dynamical membrane properties obtained by complementary optical techniques.

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Surface Functionalization of Thermoplastic Polymers for the Fabrication of Microfluidic Devices by Photoinitiated Grafting

Cited by 207 publications

References 34 publications

Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides

Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides

Surface patterning of bonded microfluidic channels

Nanostructure of supported lipid bilayers in water

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