Surface Functionalized Colloidal Microparticles for Fast Endocytotic Cell Uptake

Reibetanz, Uta; Lessig, Jaqueline; Hoyer, Jan; Neundorf, Ines

doi:10.1002/adem.200980074

Cited by 11 publications

(11 citation statements)

References 24 publications

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“…Rhodamine B isothiocyanate poly(allylamine hydrochloride) (RITC-PAH + ) was prepared by reacting PAH hydrochloride (MW = 15 kDa) and RITC at a RITC:PAH molar ratio of 20:1 per analogous reports. 63 N , N ′-Methylenebis(acrylamide) (BIS) (99%) was purchased from Acros. 2-Hydroxy-2-methyl-1-phenylpropan-1-one (DAROCUR 1173) was purchased from Ciba Specialty Chemicals (Basel, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

“…NIPAAm (97%), AMPS (97%), NVP, PEG-DA (∼99%; M n = 575 g/mol), Trizma hydrochloride (Trizma-HCl), manganese(II) chloride (MnCl 2 ), sodium bicarbonate, methyl α- d -mannopyranoside (MaM), PDADMAC + solution (MW = 100–200 kDa), PSS – (MW ≈ 70 kDa), and FITC-dextran (MW = 4, 10, 20, or 40 kDa) were obtained from Sigma-Aldrich (St. Louis, MO). Rhodamine B isothiocyanate poly(allylamine hydrochloride) (RITC-PAH + ) was prepared by reacting PAH hydrochloride (MW = 15 kDa) and RITC at a RITC:PAH molar ratio of 20:1 per analogous reports …”

Section: Methodsmentioning

confidence: 99%

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A Layer-by-Layer Approach To Retain a Fluorescent Glucose Sensing Assay within the Cavity of a Hydrogel Membrane

Locke¹,

Means²,

Dong³

et al. 2018

ACS Appl. Bio Mater.

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A continuous glucose monitoring device that resides fully in the subcutaneous tissue has the potential to greatly improve the management of diabetes. Toward this goal, we have developed a competitive binding glucose sensing assay based on fluorescently labeled PEGylated concanavalin-A (PEGylated-TRITC-ConA) and mannotetraose (APTS-MT). In the present work, we sought to contain this assay within the hollow central cavity of a cylindrical hydrogel membrane, permitting eventual subcutaneous implantation and optical probing through the skin. A “self-cleaning” hydrogel was utilized because of its ability to cyclically deswell/reswell in vivo, which is expected to reduce biofouling and therefore extend the sensor lifetime. Thus, we prepared a hollow, cylindrical hydrogel based on a thermoresponsive electrostatic double network design composed of N-isopropylacrylamide and 2-acrylamido-2-methylpropanesulfonic acid. Next, a layer-by-layer (LbL) coating was applied to the inner wall of the central cavity of the cylindrical membrane. It consisted of 5, 10, 15, 30, or 40 alternating bilayers of positively charged poly(diallyldimethylammonium chloride) and negatively charged poly(sodium 4-styrenesulfonate). With 30 bilayers, the leaching of the smaller-sized component of the assay (APTS-MT) from the membrane cavity was substantially reduced. Moreover, this LbL coating maintained glucose diffusion across the hydrogel membrane. In terms of sensor functionality, the assay housed in the hydrogel membrane cavity tracked changes in glucose concentration (0 to 600 mg/dL) with a mean absolute relative difference of ∼11%.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A Layer-by-Layer Approach To Retain a Fluorescent Glucose Sensing Assay within the Cavity of a Hydrogel Membrane

Locke¹,

Means²,

Dong³

et al. 2018

ACS Appl. Bio Mater.

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“…On the one hand, it is necessary to analyze the interaction of functionalized carriers with PMNs to prove their suitability as a transporter. In previous studies, our group could already show the phagocytotic uptake by macrophages as well as the transport and delivery of active agents into HEK293T/17 cells (19,23). Regarding microcarrier/PMN interactions, the size-and time-dependent carrier incorporation by PMNs is of high importance.…”

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confidence: 85%

Interaction, uptake, and processing of LbL-coated microcarriers by PMNs

et al. 2011

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Functionalized microcarriers or hollow capsules transporting active agents offer the opportunity for drug delivery inside cells. A promising application of these drug delivery systems is the direct transport as well as the release of immobilized antiinflammatory substances (AIS) into polymorphonuclear leukocytes (PMNs), which play a key role in the course of inflammatory processes. The intended delivery of AIS into the inflamed tissue could alleviate tissue destruction taking place during chronic inflammation, as well as facilitate the termination of these processes. In this study, the capability of functionalized CaCO 3 microcarriers as AIS transporter system targeted at PMNs is investigated. The time-dependent interaction of protamine sulfate and dextran sulfate multilayer-coated 5 lm AE 1 lm CaCO 3 carriers with PMNs, in comparison with the usage of SiO 2 carriers as monodisperse model system of defined sizes (1, 3, and 5 lm), reveals a sufficient carrier/cell interaction and uptake for coincubation periods between 2 and 24 h. Furthermore, the microcarriers are exposed to an environment simulating primary granule/phagosomal conditions after phagocytosis by means of PMN stimulation. The incubation of CaCO 3 microcarriers with cell supernatant demonstrates a partial multilayer decomposition (three to five layers) within 24 h, allowing the gradual release of AIS within the short PMN life span. This observation suggests a potential application for this drug delivery system inside immunologically active cells and may open the way to new alternatives in the treatment of chronic processes. ' 2011 International Society for Advancement of Cytometry

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“…Furthermore, specific functionalities (e.g., for stimuli responsive disassembly , ) and active agents (e.g., α1-antitrypsin, insulin, or siRNA − ) can be integrated into the multilayer structure. In a final step, the microcarrier surface properties can be controlled by choosing the appropriate terminal polyelectrolyte layer or by immobilization/specific coupling of functional molecules like polyethylene glycol (PEG), zwitterionic peptides, or antibodies. − …”

Section: Introductionmentioning

confidence: 99%

Specific Uptake of Lipid-Antibody-Functionalized LbL Microcarriers by Cells

2016

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The modular construction of Layer-by-Layer biopolymer microcarriers facilitates a highly specific design of drug delivery systems. A supported lipid bilayer (SLB) contributes to biocompatibility and protection of sensitive active agents. The addition of a lipid anchor equipped with PEG (shielding from opsonins) and biotin (attachment of exchangeable outer functional molecules) enhances the microcarrier functionality even more. However, a homogeneously assembled supported lipid bilayer is a prerequisite for a specific binding of functional components. Our investigations show that a tightly packed SLB improves the efficiency of functional components attached to the microcarrier's surface, as illustrated with specific antibodies in cellular application. Only a low quantity of antibodies is needed to obtain improved cellular uptake rates independent from cell type as compared to an antibody-functionalized loosely packed lipid bilayer or directly assembled antibody onto the multilayer. A fast disassembly of the lipid bilayer within endolysosomes exposing the underlying drug delivering multilayer structure demonstrates the suitability of LbL-microcarriers as a multifunctional drug delivery system.

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Surface Functionalized Colloidal Microparticles for Fast Endocytotic Cell Uptake

Cited by 11 publications

References 24 publications

A Layer-by-Layer Approach To Retain a Fluorescent Glucose Sensing Assay within the Cavity of a Hydrogel Membrane

A Layer-by-Layer Approach To Retain a Fluorescent Glucose Sensing Assay within the Cavity of a Hydrogel Membrane

Interaction, uptake, and processing of LbL-coated microcarriers by PMNs

Specific Uptake of Lipid-Antibody-Functionalized LbL Microcarriers by Cells

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