2015
DOI: 10.1128/iai.00767-15
|View full text |Cite
|
Sign up to set email alerts
|

Surface Glycopolymers Are Crucial for In Vitro Anti-Wall Teichoic Acid IgG-Mediated Complement Activation and Opsonophagocytosis of Staphylococcus aureus

Abstract: The cell envelopes of many Gram-positive bacteria contain wall teichoic acids (WTAs). Staphylococcus aureus WTAs are composed of ribitol phosphate (RboP) or glycerol phosphate (GroP) backbones substituted with D-alanine and N-acetyl-D-glucosamine (GlcNAc) or N-acetyl-D-galactosamine (GalNAc). Two WTA glycosyltransferases, TarM and TarS, are responsible for modifying the RboP WTA with ␣-GlcNAc and ␤-GlcNAc, respectively. We recently reported that purified human serum anti-WTA IgG specifically recognizes ␤-GlcNA… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

1
23
0

Year Published

2015
2015
2023
2023

Publication Types

Select...
4
3
2

Relationship

2
7

Authors

Journals

citations
Cited by 35 publications
(24 citation statements)
references
References 48 publications
1
23
0
Order By: Relevance
“…In fact, our data with IgG1 antibodies against WTA suggest that naturally induced antibodies against S. aureus indeed require Fc-Fc contacts to induce complement activation on the surface of bacteria. Although we used monoclonal antibodies, it is well-recognized that IgG1 antibodies against WTA are produced during an S. aureus infection in vivo (Jung et al, 2012;Lee et al, 2015;Kurokawa et al, 2016;van Dalen et al, 2019). Also, given that excessive activation of complement has been associated with clinical manifestation of several autoimmune diseases, SpA could be used to study whether autoreactive antibodies induce IgG clustering on altered host cells.…”
Section: Discussionmentioning
confidence: 99%
“…In fact, our data with IgG1 antibodies against WTA suggest that naturally induced antibodies against S. aureus indeed require Fc-Fc contacts to induce complement activation on the surface of bacteria. Although we used monoclonal antibodies, it is well-recognized that IgG1 antibodies against WTA are produced during an S. aureus infection in vivo (Jung et al, 2012;Lee et al, 2015;Kurokawa et al, 2016;van Dalen et al, 2019). Also, given that excessive activation of complement has been associated with clinical manifestation of several autoimmune diseases, SpA could be used to study whether autoreactive antibodies induce IgG clustering on altered host cells.…”
Section: Discussionmentioning
confidence: 99%
“…TarS mediated WTA β-O-GlcNAcylation has also been implicated in the induction of anti-WTA IgG-mediated complement activation and opsonophagocytosis in clinically isolated S . aureus strains [30]. We have recently published the first structure of TarM and elucidated its catalytic mechanism [31].…”
Section: Introductionmentioning
confidence: 99%
“…Specifically, the anti‐WTA β‐1,4‐GlcNAc antibodies induce complement activation and opsonophagocytosis of S . aureus strains expressing a RboP‐GlcNAc WTA (Lee et al, ). Because S .…”
Section: Discussionmentioning
confidence: 99%
“…The WTA α‐ and β‐GlcNAc modifications impact interactions of S . aureus with both innate and adaptive immune components, including mannose‐binding lectin, langerin, and antibodies (Park et al, ; Kurokawa et al, ; Lee et al, ; Gerlach et al, ; van Dalen et al, ).…”
Section: Introductionmentioning
confidence: 99%