Surface Gradient of Functional Heparin

Robinson, David; Marson, Andrew; Short, Robert D.; Buttle, David J.; Day, Anthony J.; Parry, Kristina L.; Wiles, Michelle; Highfield, Peter E.; Mistry, Anita R.; Whittle, Jason D.

doi:10.1002/adma.200702586

Cited by 71 publications

(76 citation statements)

References 29 publications

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“…This ability to plasma-treat surfaces with two monomers concurrently to facilitate conjugation to aldehyde groups while preventing physisorptive attachment elsewhere, in one step and without blocking, is a very important consideration for the fabrication of bioconjugation platforms. Since others have shown that protein adsorption to plasma polymer surfaces may result in protein denaturation, 18,29 in the final section we demonstrate that our prepared gradient surfaces exhibit retention of SAV binding capability.…”

Section: ■ Discussionmentioning

confidence: 87%

Immobilized Streptavidin Gradients as Bioconjugation Platforms

et al. 2012

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Surface density gradients of streptavidin (SAV) were created on solid surfaces and demonstrated functionality as a bioconjugation platform. The surface density of immobilized streptavidin steadily increased in one dimension from 0 to 235 ng cm(-2) over a distance of 10 mm. The density of coupled protein was controlled by its immobilization onto a polymer surface bearing a gradient of aldehyde group density, onto which SAV was covalently linked using spontaneous imine bond formation between surface aldehyde functional groups and primary amine groups on the protein. As a control, human serum albumin was immobilized in the same manner. The gradient density of aldehyde groups was created using a method of simultaneous plasma copolymerization of ethanol and propionaldehyde. Control over the surface density of aldehyde groups was achieved by manipulating the flow rates of these vapors while moving a mask across substrates during plasma discharge. Immobilized SAV was able to bind biotinylated probes, indicating that the protein retained its functionality after being immobilized. This plasma polymerization technique conveniently allows virtually any substrate to be equipped with tunable protein gradients and provides a widely applicable method for bioconjugation to study effects arising from controllable surface densities of proteins.

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Section: ■ Discussionmentioning

confidence: 87%

Immobilized Streptavidin Gradients as Bioconjugation Platforms

et al. 2012

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“…[19] Significantly, Robinson et al report that heparin can be adsorbed to the positively charged surface of plasma polymerized allylamine films based on their electrostatic interaction. [21] Such non-covalently immobilized heparin retains excellent activity and its protein-binding properties.…”

Section: Introductionmentioning

confidence: 99%

Bioactive Plasma‐Polymerized Bipolar Films for Enhanced Endothelial Cell Mobility

Yang

Wang

et al. 2011

Macromolecular Bioscience

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A facile approach to a highly bio-active interface material is reported. XPS reveals that polar entities exist at the interface between PPAam and PPAac nanolayers. They induce strong dipolar orientation polarizability and cause the redistribution of charges, which results in a remarkable increase of polar surface energy and hydrophilicity of the multistack bipolar films. In particular bipolar films with amine groups on their outermost surface show strongly enhanced cellular mobility. The attachment, adhesion, proliferation, migration, and coverage of ECs are significantly enhanced on such films. They are therefore promising as vascular implant materials, and could have applications as coating materials for tissue engineering.

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“…The biotinylated Link module from human TSG-6 (bA-Link_TSG6 (26) denoted here as GAG-BP), a well characterized GAG-binding domain (27), was used in a colorimetric assay to determine the amount of functional heparin/HS adsorbed onto 0.6-cm 2 uncoated and ppAm scaffolds as described previously for two-dimensional surfaces (20–22, 28). For details, see supplemental “Experimental Procedures.”…”

Section: Methodsmentioning

confidence: 99%

Immobilization of Heparan Sulfate on Electrospun Meshes to Support Embryonic Stem Cell Culture and Differentiation *

Meade

White

Pickford

et al. 2013

Journal of Biological Chemistry

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Background: Glycosaminoglycans influence stem cell fate but their combination with biomaterials remains to be optimized.Results: GAG bound to scaffolds presented essential sulfation epitopes and proved biologically active.Conclusion: Use of plasma polymerized allylamine proved effective in functionalizing a fibrous extracellular matrix mimic.Significance: The biomaterial has broad applicability to stem cell culture and has potential future applications in regenerative medicine.

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Surface Gradient of Functional Heparin

Cited by 71 publications

References 29 publications

Immobilized Streptavidin Gradients as Bioconjugation Platforms

Immobilized Streptavidin Gradients as Bioconjugation Platforms

Bioactive Plasma‐Polymerized Bipolar Films for Enhanced Endothelial Cell Mobility

Immobilization of Heparan Sulfate on Electrospun Meshes to Support Embryonic Stem Cell Culture and Differentiation *

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