Surface Infection with Pseudomonas Aeruginosa

Walker, Harrel L.; Mason, Arthur D.; Raulston, Gilbert L.

doi:10.1097/00000658-196408000-00019

Cited by 87 publications

(30 citation statements)

References 2 publications

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“…Thermal injury. The procedures utilized to burn the rats were adapted from those described by Walker et al (25). Rats were anesthetized intraperitoneally with 25 mg of pentobarbital sodium (Nembutal) per kg of body weight.…”

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Bacterial translocation from the gastrointestinal tracts of rats receiving thermal injury

Maejima¹,

Deitch²,

Berg³

1984

Infect Immun

175

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Rats receiving nonlethal thermal burns over 20 or 40% of their total body surface area were tested at various intervals for the translocation of indigenous bacteria from their gastroititestinal tracts to their mesenteric lymph nodes, peritoneal cavities, and bloodstreams. No indigenous bacteria were cultured from these organs of control rats or from rats receiving 20% burns. However, 44%Y of the rats receiving 40% burns exhibited viable Escherichia coli, Proteus mirabilis, Staphylococcus sp. and Clostridium sp. in their mesenteric lymph nodes 2 days after thermal injury. Bacterial translocation after burn stress also was tested in antibiotic-decontaminated rats monoassociated with E. coli. E. (oli attained population levels in these animals of 108 to 109 per g of cecum. E. (coli translocated to 100% of the mesenteric lymph nodes of both the control and 40% burned rats. However, E. coli translocated at a greater incidence to the spleens, livers, and peritoneal cavities of the burned rats compared with translocation to these organs in control rats. The numbers of E. coli translocating to the mesenteric lymph nodes, spleens, and livers also were greater in the 40% burned rats than in control rats. By 14 days after thermal injury, the rats were able to clear E. coli from their spleens and livers, and the infection remained localized in the mesenteric lymph nodes. These results support the concept that the indigenous gastrointestinal flora or exogenous organisms colonizing the gastrointestinal tract are potential sources of septicemia after thermal injury.

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confidence: 99%

Bacterial translocation from the gastrointestinal tracts of rats receiving thermal injury

Maejima¹,

Deitch²,

Berg³

1984

Infect Immun

175

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“…There have been many reports of the development of animal models of infected thermal burns with both mice and rats (29,39,40). As mentioned above, it was necessary to apply the bacteria to the surface of the burn in order to test the effects of the antimicrobial dressings.…”

Section: Discussionmentioning

confidence: 99%

Chitosan Acetate Bandage as a Topical Antimicrobial Dressing for Infected Burns

Tegos

Burkatovskaya

Castaño

et al. 2009

Antimicrob Agents Chemother

117

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An engineered chitosan acetate bandage preparation (HemCon) is used as a hemostatic dressing, and its chemical structure suggests that it should also be antimicrobial. We previously showed that when a chitosan acetate bandage was applied to full-thickness excisional wounds in mice that had been infected with pathogenic bioluminescent bacteria (Pseudomonas aeruginosa, Proteus mirabilis, and Staphylococcus aureus), it was able to rapidly kill the bacteria and save the mice from developing fatal infections. Wound healing was also stimulated. In the present study, we asked whether a chitosan acetate bandage could act as a topical antimicrobial dressing when it was applied to third-degree burns in mice contaminated with two of these bacterial species (P. aeruginosa and P. mirabilis). Preliminary experiments established the length of burn time and the number of bacteria needed to produce fatal infections in untreated mice and established that the chitosan acetate bandage could adhere to the infected burn for up to 21 days. In the case of P. aeruginosa infections, the survival rate of mice treated with the chitosan acetate bandage was 73.3% (whereas the survival rate of mice treated with a nanocrystalline silver dressing was 27.3% [P ‫؍‬ 0.0055] and that of untreated mice was 13.3% [P < 0.0002]). For P. mirabilis infections, the comparable survival rates were 66.7%, 62.5%, and 23.1% respectively. Quantitative bioluminescent signals showed that the chitosan acetate bandage effectively controlled the growth of bacteria in the burn and prevented the development of systemic sepsis, as shown by blood culture. These data suggest that chitosan acetate bandage is efficacious in preventing fatal burn infections.

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“…In fact, when this is recapitulated in controlled experiments whereby anesthetized animals undergo a severe burn and inoculation with high numbers of virulent organisms while other similarly treated animals are not inoculated, septic shock and its ramifications are found in the inoculated animals, thus demonstrating a clear association of high numbers of organisms in a burn wound and septic shock [1]. When these organisms are controlled with debridement or topical anti-microbials such that high numbers of organisms are no longer present, thus removing the factor, the ramifications of septic shock (effect) disappear [2,3].…”

Section: Introductionmentioning

confidence: 96%