Surface-Initiated Polymer Brushes in the Biomedical Field: Applications in Membrane Science, Biosensing, Cell Culture, Regenerative Medicine and Antibacterial Coatings

Krishnamoorthy, Mahentha; Hakobyan, Shoghik; Ramstedt, Madeleine; Gautrot, Julien E.

doi:10.1021/cr500252u

Cited by 548 publications

(525 citation statements)

References 614 publications

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“…), is becoming an optimized immobilization method for microarray fabrications (14)(15)(16). Meanwhile, due to its high loading capacity for biomolecules and antifouling properties, 3D polymer surface with a designed structure often shows high signal-to-noise ratio in SPRi (17)(18)(19). Theoretically, a carbene-based 3D surface could immobilize biomolecules randomly in position and orientation to best retain the functionality and, in the meantime, has the advantage of high sensitivity and low nonspecific adsorption.…”

Section: Significancementioning

confidence: 99%

Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology

Zhao

Yang

Zhou

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Chemical modifications on histones and DNA/RNA constitute a fundamental mechanism for epigenetic regulation. These modifications often function as docking marks to recruit or stabilize cognate "reader" proteins. So far, a platform for quantitative and high-throughput profiling of the epigenetic interactome is urgently needed but still lacking. Here, we report a 3D-carbene chipbased surface plasmon resonance imaging (SPRi) technology for this purpose. The 3D-carbene chip is suitable for immobilizing versatile biomolecules (e.g., peptides, antibody, DNA/RNA) and features low nonspecific binding, random yet function-retaining immobilization, and robustness for reuses. We systematically profiled binding kinetics of 1,000 histone "reader-mark" pairs on a single 3D-carbene chip and validated two recognition events by calorimetric and structural studies. Notably, a discovery on H3K4me3 recognition by the DNA mismatch repair protein MSH6 in Capsella rubella suggests a mechanism of H3K4me3-mediated DNA damage repair in plant. The mechanisms leading to differential transcriptional and developmental outcomes under the same genetic background are known as epigenetics. Posttranslational modification (PTM) on histones is a major epigenetic regulatory mechanism such that more than 30 types of PTMs occur on histones, with new ones still being discovered (1). The well-studied histone PTMs include methylation, acetylation, phosphorylation, and most recently discovered nonacetyl acylations (2). Histone PTMs regulate transcription either by directly affecting the structure/stability of single nucleosome and the high-order folding of chromatin, or by recruiting specific effector proteins (also called histone readers) recognizing them (3, 4).The ever-expanding repertoire of histone readers are diverse in sequence and structure, and how they engage diversely modified chromatin is complicated, hard to predict, yet important for their functions. Often one type of reader domains can recognize different types of PTMs. For example, PHD finger proteins can recognize unmodified, methylated, or acylated lysine residue on histones (5, 6). Several YEATS domain proteins, originally identified as readers of lysine acetylation (Kac), in fact accommodate a wide range of lysine acylations with lysine crotonylation (Kcr) most preferred (7). These "mark-reader" interactions vary greatly in binding affinity, ranging from submicromolar to millimolar levels. To complicate the matter further, the recognition of marks by reader proteins is often either synergized or antagonized by other marks in close proximity (8). For instance, Spindlin1 recognizes histone H3 trimethylation of Lys-4 (H3K4me3) and asymmetrically dimethylation of Arg-8 (H3R8me2a) in concert, while phosphorylation of Ser-10 on H3 (H3S10ph) completely abrogates the binding of H3 Lys-9 trimethylation (H3K9me3) by HP1 protein (9, 10). BRDT protein binds neither histone H4 Lys-5 acetylation (H4K5ac) nor Lys-8 acetylation (H4K8ac) but engages H4 peptide bearing both marks with decent affinity (K...

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Section: Significancementioning

confidence: 99%

Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology

Zhao

Yang

Zhou

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…This section will focus on the recent advances using polymer brushes in antifouling, biosensor, implant, and defined cell culture applications and more specifically their use as low fouling materials for selective binding of proteins, cells, antigens and tissues. For a more in depth review on biomedical applications of polymer brushes, there have been several excellent published reviews [154][155][156]. …”

Section: Key Applications: Imparting Functionalities In Polymer Brushmentioning

confidence: 99%

From Self-Assembled Monolayers to Coatings: Advances in the Synthesis and Nanobio Applications of Polymer Brushes

et al. 2015

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Abstract:In this review, we describe the latest advances in synthesis, characterization, and applications of polymer brushes. Synthetic advances towards well-defined polymer brushes, which meet criteria such as: (i) Efficient and fast grafting, (ii) Applicability on a wide range of substrates; and (iii) Precise control of surface initiator concentration and hence, chain density are discussed. On the characterization end advances in methods for the determination of relevant physical parameters such as surface initiator concentration and grafting density are discussed. The impact of these advances specifically in emerging fields of nano-and bio-technology where interfacial properties such as surface energies are controlled to create nanopatterned polymer brushes and their implications in mediating with biological systems is discussed.

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“…[1][2][3][4] A popular example of a thermoresponsive polymer is poly(Nisopropylacrylamide) (PNIPAAm), which has been extensively studied in solution as well as in the form of thin layers on a substrate. [5][6][7] In the past years, another class of temperature-responsive polymers has been used to fabricate biocompatible switchable surfaces, namely, poly(2-alkyl-2-oxazoline)s (POx).…”

Section: Introductionmentioning

confidence: 99%

Probing carbonyl–water hydrogen-bond interactions in thin polyoxazoline brushes

et al. 2016

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Temperature-responsive oxazoline-based polymer brushes have gained increased attention as biocompatible surfaces. In aqueous environment, they can be tuned between hydrophilic and hydrophobic behavior triggered by a temperature stimulus. This transition is connected with changes in molecule–solvent interactions and results in a switching of the brushes between swollen and collapsed states. This work studies the temperature-dependent interactions between poly(2-oxazoline) brushes and water. In detail, thermoresponsive poly(2-cyclopropyl-2-oxazoline), nonresponsive hydrophilic poly(2-methyl-2-oxazoline), as well as a copolymer of the two were investigated with in situ infrared ellipsometry. Focus was put on interactions of the brushes' carbonyl groups with water molecules. Different polymer–water interactions could be observed and assigned to hydrogen bonding between C=O groups and water molecules. The switching behavior of the brushes in the range of 20–45 °C was identified by frequency shifts and intensity changes of the amide I band.

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Surface-Initiated Polymer Brushes in the Biomedical Field: Applications in Membrane Science, Biosensing, Cell Culture, Regenerative Medicine and Antibacterial Coatings

Cited by 548 publications

References 614 publications

Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology

Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology

From Self-Assembled Monolayers to Coatings: Advances in the Synthesis and Nanobio Applications of Polymer Brushes

Probing carbonyl–water hydrogen-bond interactions in thin polyoxazoline brushes

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