Dietary variability impacts food web dynamics and resource partitioning among individuals and species in a community. However, it is difficult to characterize the ecological niche of sharks, a mobile and long-lived predator. Stable isotope analysis allows the quantification of niche width since it incorporates both environmental and biological variation. Carbon isotope composition varies between marine productivity regimes while nitrogen isotope composition indicates trophic position, but baseline can vary with seasonal or regional shifts. We sampled dental collagen for stable isotope analysis. The conveyor-like growth of shark teeth provides a time series allowing for multiple measurements per individuals. We sampled 59 individuals from 11 species captured in 2005 at Suruga Bay, Japan (34°50'59.99" N 138°32'59.99" E). We estimated three ecological metrics based on stable isotope composition—standard ellipse area, convex hull area, and Pianka’s measure—which elucidated the extent of ecological variation and allowed comparisons among individuals and species. Our results show a significant positive correlation between dental collagen δ15N values and total length (r2 = 0.23; p = 2⨉10− 16), but individual variability had a low coefficient of determination. The variation in isotopic niche within and among individuals as well between species across all habitat types suggest that many species spanned the generalist – specialist continuum. Our findings indicate there are subtle ecological differences between species and among individuals; multiple measurements of carbon and nitrogen composition from multiple teeth series indicate variable ecological niches. Comparing patterns across habitats suggests sharks have complex food web dynamics with extensive individual- and species-level variation.