Surface layer protein A from hypervirulent Clostridioides difficile ribotype 001 can induce autophagy process in human intestinal epithelial cells

Amirkamali, Sahar; Azimirad, Masoumeh; Nasiri, Gelareh; Goudarzi, Hossein; Noori, Maryam; Yadegar, Abbas; Ghalavand, Zohreh; Zali, Mohammad Reza

doi:10.1016/j.micpath.2022.105681

Cited by 4 publications

(1 citation statement)

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“…We also saw an increase in expression for autophagy related genes in C. elegans fed with pathogenic C. difficile (Figure 3E). Recent articles have also highlighted a comparable occurrence in different model systems where the host responds to toxins produced by C. difficile 49,50 . Apart from this we also saw an increase in expression for NLP-29, HLH-30, EGL-5, SCAV-3, CED-1, XBP-1, TOL-1, ELT-3, FSHR-1, and SKN-1 (Figure S8C).…”

Section: Continuous Feeding Of C Elegans With B Longum Enhances While...mentioning

confidence: 92%

ACaenorhabditis elegansbased system for high-throughput functional phenotyping of human gut microbiota

Ambat,

Valappil,

Ghimire

et al. 2024

Preprint

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SummaryBottoms-up approach of mono or poly colonizing microbes in germfree model is an important tool for mechanistic understanding of human gut microbiota. However, doing this in models such as germfree mouse is expensive and time consuming. To address this problem, we developed aCaenorhabditis elegansbased screening system. We used a gut microbiota culture collection that represents more than 70% functional capacity of the human gut microbiome to anaerobically colonizeC. elegans. We chose colonization resistance as the phenotype of the microbiome for further screening and found that half of the strains, previously identifiedin vitroas inhibitingClostridioides difficile, also did so inC. elegans. When validated using germ-free mouse model, results were in concordance with that obtained fromC. elegansmodel. Our system therefore could be used for screening large number of bacterial species to better understand host-microbiome interaction.

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Section: Continuous Feeding Of C Elegans With B Longum Enhances While...mentioning

confidence: 92%

ACaenorhabditis elegansbased system for high-throughput functional phenotyping of human gut microbiota

Ambat,

Valappil,

Ghimire

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

The modulatory effect of Lactobacillus gasseri ATCC 33323 on autophagy induced by extracellular vesicles of Helicobacter pylori in gastric epithelial cells in vitro

Sadeghloo,

Saffarian,

Hakemi-Vala

et al. 2024

Microbial Pathogenesis

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The interplay between probiotics and host autophagy: mechanisms of action and emerging insights

Sadeghloo,

Nabavi-Rad,

Zali

et al. 2024

Autophagy

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Surface layer protein A from hypervirulent Clostridioides difficile ribotype 001 can induce autophagy process in human intestinal epithelial cells

Cited by 4 publications

References 42 publications

ACaenorhabditis elegansbased system for high-throughput functional phenotyping of human gut microbiota

ACaenorhabditis elegansbased system for high-throughput functional phenotyping of human gut microbiota

The modulatory effect of Lactobacillus gasseri ATCC 33323 on autophagy induced by extracellular vesicles of Helicobacter pylori in gastric epithelial cells in vitro

The interplay between probiotics and host autophagy: mechanisms of action and emerging insights

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